Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   P7I96_RS30860 Genome accession   NZ_CP124660
Coordinates   6530437..6531060 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain 2022CK-00160     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6525437..6536060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7I96_RS30840 (P7I96_30840) aroB 6526032..6527138 (-) 1107 WP_003162482.1 3-dehydroquinate synthase -
  P7I96_RS30845 (P7I96_30845) aroK 6527188..6527706 (-) 519 WP_003095833.1 shikimate kinase AroK -
  P7I96_RS30850 (P7I96_30850) pilQ 6527718..6529862 (-) 2145 WP_014603377.1 type 4a pilus secretin PilQ Machinery gene
  P7I96_RS30855 (P7I96_30855) comL/pilP 6529916..6530440 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  P7I96_RS30860 (P7I96_30860) pilO 6530437..6531060 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  P7I96_RS30865 (P7I96_30865) pilN 6531057..6531653 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  P7I96_RS30870 (P7I96_30870) pilM 6531653..6532042 (-) 390 Protein_6095 type IV pilus assembly protein PilM -
  P7I96_RS30880 (P7I96_30880) pilM 6533707..6534387 (-) 681 Protein_6097 type IV pilus assembly protein PilM -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=760306 P7I96_RS30860 WP_003103268.1 6530437..6531060(-) (pilO) [Pseudomonas aeruginosa strain 2022CK-00160]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=760306 P7I96_RS30860 WP_003103268.1 6530437..6531060(-) (pilO) [Pseudomonas aeruginosa strain 2022CK-00160]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAGCAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401