Detailed information NTG0759876

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OSH17_RS13445
• Genome accession: NZ_CP113073
• Coordinates: 2872541..2873659 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain A118 DeltaH0N27_10820::kan H0N27_10830-K176N
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2867541..2878659

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OSH17_RS13435 (OSH17_13435)	-	2870657..2871349 (-)	693	WP_168726751.1	YggS family pyridoxal phosphate-dependent enzyme	-
OSH17_RS13440 (OSH17_13440)	pilT	2871476..2872513 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
OSH17_RS13445 (OSH17_13445)	pilU	2872541..2873659 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OSH17_RS13450 (OSH17_13450)	fur	2873729..2874166 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
OSH17_RS13455 (OSH17_13455)	bamE	2874278..2874676 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
OSH17_RS13460 (OSH17_13460)	-	2874731..2875054 (-)	324	WP_045901052.1	RnfH family protein	-
OSH17_RS13465 (OSH17_13465)	-	2875054..2876121 (-)	1068	WP_168726750.1	hypothetical protein	-
OSH17_RS13470 (OSH17_13470)	-	2876274..2876729 (-)	456	WP_000782976.1	bacteriohemerythrin	-
OSH17_RS13475 (OSH17_13475)	-	2876982..2877830 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=759876 OSH17_RS13445 WP_000347034.1 2872541..2873659(+) (pilU) [Acinetobacter baumannii strain A118 DeltaH0N27_10820::kan H0N27_10830-K176N]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=759876 OSH17_RS13445 WP_000347034.1 2872541..2873659(+) (pilU) [Acinetobacter baumannii strain A118 DeltaH0N27_10820::kan H0N27_10830-K176N]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGCCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TTATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAATGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCACAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39