Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ORI57_RS05280 Genome accession   NZ_CP111081
Coordinates   1130342..1131727 (-) Length   461 a.a.
NCBI ID   WP_078801766.1    Uniprot ID   -
Organism   Pasteurella multocida strain PF2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1125342..1136727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ORI57_RS05250 (ORI57_05225) - 1125469..1126842 (-) 1374 WP_005722571.1 sodium:alanine symporter family protein -
  ORI57_RS05255 (ORI57_05230) - 1127291..1127560 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  ORI57_RS05260 (ORI57_05235) yacG 1127560..1127763 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  ORI57_RS05265 (ORI57_05240) coaE 1127774..1128394 (-) 621 WP_010906475.1 dephospho-CoA kinase -
  ORI57_RS05270 (ORI57_05245) - 1128409..1129131 (-) 723 WP_010906474.1 A24 family peptidase -
  ORI57_RS05275 (ORI57_05250) - 1129131..1130342 (-) 1212 WP_005722560.1 type II secretion system F family protein -
  ORI57_RS05280 (ORI57_05255) pilB 1130342..1131727 (-) 1386 WP_078801766.1 GspE/PulE family protein Machinery gene
  ORI57_RS05285 (ORI57_05260) pilA 1131727..1132161 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  ORI57_RS05290 (ORI57_05265) ampD 1132321..1132887 (+) 567 WP_225529746.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ORI57_RS05295 (ORI57_05270) rppH 1133472..1134068 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  ORI57_RS05300 (ORI57_05275) - 1134071..1134865 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  ORI57_RS05305 (ORI57_05280) lgt 1134875..1135687 (+) 813 WP_010906471.1 prolipoprotein diacylglyceryl transferase -
  ORI57_RS05310 (ORI57_05285) - 1135684..1136535 (+) 852 WP_010906470.1 thymidylate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52063.74 Da        Isoelectric Point: 6.3686

>NTDB_id=757728 ORI57_RS05280 WP_078801766.1 1130342..1131727(-) (pilB) [Pasteurella multocida strain PF2]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLL
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFGELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRCCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=757728 ORI57_RS05280 WP_078801766.1 1130342..1131727(-) (pilB) [Pasteurella multocida strain PF2]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGCGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGTTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAGGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACTA
GTCCAGCCTTCCTTTTCAGGGATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACACATTTAGGCGAAAAAGCGGTATTGCGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGGTGAGTTGGGGATG
ACCGCAGACCAACAACACAGATTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGTTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCAGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCGCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTCTTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTTGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.77

98.265

0.666
  pilB Haemophilus influenzae Rd KW20

67.329

98.265

0.662
  pilB Glaesserella parasuis strain SC1401

58.811

98.482

0.579
  pilB Acinetobacter baumannii D1279779

40.202

100

0.432
  pilB Acinetobacter baylyi ADP1

37.876

100

0.41
  pilB Vibrio parahaemolyticus RIMD 2210633

39.293

100

0.41
  pilB Vibrio campbellii strain DS40M4

39.04

100

0.406
  pilB Vibrio cholerae strain A1552

38.272

100

0.403
  pilB Legionella pneumophila strain ERS1305867

37.219

100

0.395