Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PHLH3_RS27805 Genome accession   NZ_AP021905
Coordinates   6097551..6098615 (-) Length   354 a.a.
NCBI ID   WP_173171164.1    Uniprot ID   -
Organism   Pseudomonas sp. Pc102     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6092551..6103615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PHLH3_RS27780 (PHLH8_55140) aroK 6093173..6093691 (-) 519 WP_173171174.1 shikimate kinase AroK -
  PHLH3_RS27785 (PHLH8_55150) pilQ 6093697..6095775 (-) 2079 WP_213626735.1 type IV pilus secretin PilQ Machinery gene
  PHLH3_RS27790 (PHLH8_55160) pilP 6095829..6096362 (-) 534 WP_213626737.1 type 4a pilus biogenesis lipoprotein PilP -
  PHLH3_RS27795 (PHLH8_55170) pilO 6096359..6096982 (-) 624 WP_213626739.1 type 4a pilus biogenesis protein PilO -
  PHLH3_RS27800 (PHLH8_55180) pilN 6096979..6097551 (-) 573 WP_213626741.1 type 4a pilus biogenesis protein PilN -
  PHLH3_RS27805 (PHLH8_55190) pilM 6097551..6098615 (-) 1065 WP_173171164.1 pilus assembly protein PilM Machinery gene
  PHLH3_RS27810 (PHLH8_55200) - 6098800..6101220 (+) 2421 WP_213630707.1 penicillin-binding protein 1A -
  PHLH3_RS27815 (PHLH8_55210) - 6101334..6102602 (-) 1269 WP_173171162.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37680.91 Da        Isoelectric Point: 4.4429

>NTDB_id=75764 PHLH3_RS27805 WP_173171164.1 6097551..6098615(-) (pilM) [Pseudomonas sp. Pc102]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLGKAKASSKSVAV
AVAGSAVITKTIEMDAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGASARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLAAQLGNGSDELTVAVVDIGATMTTLSVLHNGRTIYSREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTQVANP
FAEMALSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=75764 PHLH3_RS27805 WP_173171164.1 6097551..6098615(-) (pilM) [Pseudomonas sp. Pc102]
GTGCTAGGGCTCTTCAACAAGAAAGCGAATACGCTGCTGGGGATCGACATAAGCTCGACTTCAGTCAAGCTCCTTGAATT
GAGTCGCTCGGGAAGCCGCTACAAGGTAGAGGCCTACGCCGTCGAGCCGCTCCCACCGAATGCTGTGGTCGAAAAGAACA
TCGCCGAACTGGAGGGGGTCGGCCAGGCGTTGTCTCGTGTTCTTGGCAAGGCCAAGGCCAGTTCGAAGTCCGTCGCCGTA
GCGGTCGCGGGCTCCGCTGTAATCACCAAGACGATCGAGATGGATGCCGGTCTTTCCGACGATGAGTTGGAGAACCAGCT
GAAGATCGAGGCGGACCAGTACATCCCTTATCCGCTCGAAGAAGTCGCGATCGACTTCGAGGTCCAGGGCGCTTCCGCGC
GCAACCCTGAGCGGGTCGATGTGCTCCTCGCCGCGTGCCGCAAGGAGAACGTCGAGGTCCGTGAAGCCGCTCTGGCCCTG
GCGGGCCTGACCGCCAAGGTCGTCGACGTCGAAGCCTATGCGCTGGAACGTGCCTACGGACTTCTCGCTGCGCAACTGGG
CAACGGGAGCGACGAACTGACGGTCGCCGTCGTGGATATCGGGGCGACCATGACGACCCTGAGCGTCCTGCACAACGGCC
GGACCATCTACAGCCGCGAGCAGCTTTTCGGCGGACGCCAGCTCACCGAGGAGATCCAGCGCCGCTATGGCCTGTCCGTG
GAAGAAGCGGGTCTTGCCAAGAAGCAGGGTGGTCTTCCGGATGACTACGACAGCGAAGTGTTGCAACCGTTCAAGGACGC
AGTGGTCCAGCAAGTGTCTCGTTCGCTGCAGTTCTTCTTCGCTGCGGGCCAGTTCAACGATGTCGACTACATCCTCCTGG
CGGGTGGCACGGCGTCCATTCCTGATCTGGATCGTTTGATCCAGCAGAAGATCGGCACTCCTACCCAGGTTGCCAACCCC
TTCGCGGAGATGGCGCTGAGTGGCAAGGTCAATGCGGGCGCATTGGCCAGTGATGCCCCGGCGCTGATGATTGCCTGCGG
ACTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

57.062

100

0.571
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  comM Acinetobacter baylyi ADP1

53.39

100

0.534
  pilM Legionella pneumophila strain ERS1305867

47.74

100

0.477

Multiple sequence alignment