Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KI231_RS16620 Genome accession   NZ_AP021903
Coordinates   3712525..3713637 (-) Length   370 a.a.
NCBI ID   WP_212809135.1    Uniprot ID   -
Organism   Pseudomonas sp. Seg1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3707525..3718637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI231_RS16605 (PHLH6_32820) - 3708330..3709721 (+) 1392 WP_077573126.1 GntP family permease -
  KI231_RS16610 (PHLH6_32830) hbdH 3709729..3710502 (+) 774 WP_103302337.1 3-hydroxybutyrate dehydrogenase -
  KI231_RS16615 (PHLH6_32840) - 3710565..3712520 (+) 1956 WP_212809134.1 acetoacetate--CoA ligase -
  KI231_RS16620 (PHLH6_32850) pilU 3712525..3713637 (-) 1113 WP_212809135.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KI231_RS16625 (PHLH6_32860) - 3713766..3716303 (+) 2538 WP_212809136.1 PAS domain-containing sensor histidine kinase -
  KI231_RS16630 (PHLH6_32870) - 3716355..3716630 (+) 276 WP_025112520.1 peptidylprolyl isomerase -
  KI231_RS16635 (PHLH6_32880) - 3716743..3717675 (-) 933 WP_212809137.1 sugar kinase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41276.21 Da        Isoelectric Point: 6.6994

>NTDB_id=75681 KI231_RS16620 WP_212809135.1 3712525..3713637(-) (pilU) [Pseudomonas sp. Seg1]
MEIDALLKQLASRHGSDLFLSTGAPPSARFDGVLTPLSEQPFKPGELATVATSLMDAEQRREFDRDLEMNLAISRTGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPAILLETVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIVTIED
PIEYIHRHQRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFAETGHLVLSTLHANNANQALDRI
TNMFTEERRPQLLHALGNNLRAFVSQRLVRTLDGQRRAAVEVMLGTPTVTDLIRRNEFTELKGIMEKSAELGMQTFDAAL
FALVVEKVISEEEALKHADSVNNLRLRLKLHDDAGPGLPPSSSGEWGLMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=75681 KI231_RS16620 WP_212809135.1 3712525..3713637(-) (pilU) [Pseudomonas sp. Seg1]
ATGGAAATCGACGCACTGCTTAAACAACTGGCAAGCCGGCACGGTTCGGATCTGTTTCTGTCCACGGGCGCGCCACCCAG
TGCAAGGTTCGACGGCGTGCTCACGCCGCTAAGCGAGCAACCCTTCAAACCCGGCGAGTTGGCGACTGTCGCCACCTCAC
TGATGGACGCCGAGCAGCGCCGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCGATCTCGCGTACCGGGATTGGCCGC
TTCCGGGTCAACATTTTCAAACAGCGCAATGATGTGTCGATCGTGATCCGTAACGTCAAACTCGACATCCCGCGTTTCGA
AGATTTGAAGCTGCCGGCCATACTGCTTGAAACCGTGATGCTCAAACAGGGCCTGATCCTGTTTGTCGGCGCCACCGATT
CCGGTAAGTCGACGTCATTGGCAGCGCTTATCGACCACCGCAATCGCCATAGTAGCGGGCACATCGTCACCATCGAAGAC
CCGATCGAGTACATCCACCGGCATCAACGCTCAATCATCAACCAGCGAGAAGTCGGCGTCGACACCCGCAGTTTTCACGC
TGCGCTGAAAAACACTCTGCGCCAGGCGCCGGACGTGGTGCTGATCGGCGAAATTCGTGACCGCGAAACCATGGAGCATG
CGCTGGCGTTTGCCGAGACCGGGCATCTGGTGCTTTCAACCCTGCATGCAAATAACGCCAATCAGGCGCTGGACCGGATC
ACCAACATGTTCACCGAAGAGCGTCGCCCGCAGTTGTTGCATGCGCTGGGCAACAACCTGAGGGCCTTTGTCTCACAGCG
GTTGGTGCGCACGCTGGACGGACAGCGCAGGGCCGCGGTCGAGGTCATGCTGGGTACACCGACAGTGACGGATCTGATTC
GTCGAAACGAATTCACCGAGTTGAAGGGCATCATGGAGAAGTCTGCCGAGCTTGGCATGCAGACGTTTGATGCCGCACTG
TTTGCGCTGGTGGTGGAGAAAGTGATCAGTGAGGAGGAGGCGCTCAAACATGCGGACTCGGTAAACAACCTGCGGTTGCG
TTTGAAATTGCATGACGATGCCGGCCCCGGGTTGCCGCCATCCTCCTCGGGTGAGTGGGGATTGATGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(58-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.695

94.865

0.538
  pilU Acinetobacter baylyi ADP1

53.889

97.297

0.524
  pilU Vibrio cholerae strain A1552

50.278

97.297

0.489
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.118

98.108

0.384

Multiple sequence alignment