Detailed information NTG0756637

Bioinformatically predicted

Overview

• Name: comGB
• Type: Machinery gene
• Locus tag: OHI04_RS11385
• Genome accession: NZ_CP110849
• Coordinates: 2254261..2255334 (-)
• Length: 357 a.a.
• NCBI ID: WP_080756637.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain VHProbi V60
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2249261..2260334

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OHI04_RS11345 (OHI04_11350)	-	2249623..2250432 (-)	810	WP_014570791.1	metal ABC transporter permease	-
OHI04_RS11350 (OHI04_11355)	-	2250425..2251162 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
OHI04_RS11355 (OHI04_11360)	-	2251339..2252181 (-)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
OHI04_RS11360 (OHI04_11365)	-	2252178..2252615 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
OHI04_RS11365 (OHI04_11370)	comGG	2252697..2252981 (-)	285	WP_025017139.1	competence type IV pilus minor pilin ComGG	Machinery gene
OHI04_RS11370 (OHI04_11375)	comGF	2253020..2253502 (-)	483	WP_039116460.1	competence type IV pilus minor pilin ComGF	Machinery gene
OHI04_RS11375 (OHI04_11380)	comGD	2253474..2253833 (-)	360	WP_023188582.1	competence type IV pilus minor pilin ComGD	Machinery gene
OHI04_RS11380 (OHI04_11385)	comGC	2253865..2254134 (-)	270	WP_023188581.1	competence type IV pilus major pilin ComGC	Machinery gene
OHI04_RS11385 (OHI04_11390)	comGB	2254261..2255334 (-)	1074	WP_080756637.1	competence type IV pilus assembly protein ComGB	Machinery gene
OHI04_RS11390 (OHI04_11395)	comGA	2255228..2256165 (-)	938	Protein_2213	competence type IV pilus ATPase ComGA	-

Sequence

Protein

• Length: 357 a.a.
• Molecular weight: 40367.57 Da
• Isoelectric Point: 10.3001

>NTDB_id=756637 OHI04_RS11385 WP_080756637.1 2254261..2255334(-) (comGB) [Lactococcus lactis strain VHProbi V60]
MSLGHQKIGIKKLISYLQMDISHLLKPKGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLA
SGQVLSEILESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEHFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide

• Length: 1074 bp

>NTDB_id=756637 OHI04_RS11385 WP_080756637.1 2254261..2255334(-) (comGB) [Lactococcus lactis strain VHProbi V60]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAAAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTACATTAAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGTTTTATCAGAGATTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGAACGATGCAATTGGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACATTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCATTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACATTTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCTGGAATATAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (41-159)

• Type II secretion system (T2SS), protein F (T2SSF) (227-348)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGB	Lactococcus lactis subsp. cremoris KW2	83.578	95.518	0.798
comYB	Streptococcus gordonii str. Challis substr. CH1	53.959	95.518	0.515
comYB	Streptococcus mutans UA140	53.959	95.518	0.515
comYB	Streptococcus mutans UA159	53.666	95.518	0.513
comGB/cglB	Streptococcus mitis SK321	51.343	93.838	0.482
comGB/cglB	Streptococcus pneumoniae TIGR4	51.045	93.838	0.479
comGB/cglB	Streptococcus pneumoniae Rx1	51.045	93.838	0.479
comGB/cglB	Streptococcus pneumoniae D39	51.045	93.838	0.479
comGB/cglB	Streptococcus pneumoniae R6	51.045	93.838	0.479
comGB/cglB	Streptococcus mitis NCTC 12261	50.448	93.838	0.473