Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PALA52_RS26000 Genome accession   NZ_CP110347
Coordinates   5644030..5646174 (-) Length   714 a.a.
NCBI ID   WP_024916548.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5639030..5651174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA52_RS25985 (PALA52_05154) - 5640694..5642337 (-) 1644 WP_121130557.1 AAA family ATPase -
  PALA52_RS25990 (PALA52_05155) aroB 5642344..5643450 (-) 1107 WP_058140600.1 3-dehydroquinate synthase -
  PALA52_RS25995 (PALA52_05156) aroK 5643500..5644018 (-) 519 WP_003095833.1 shikimate kinase AroK -
  PALA52_RS26000 (PALA52_05157) pilQ 5644030..5646174 (-) 2145 WP_024916548.1 type 4a pilus secretin PilQ Machinery gene
  PALA52_RS26005 (PALA52_05158) pilP 5646228..5646752 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  PALA52_RS26010 (PALA52_05159) pilO 5646749..5647372 (-) 624 WP_024916549.1 type 4a pilus biogenesis protein PilO -
  PALA52_RS26015 (PALA52_05160) pilN 5647369..5647968 (-) 600 WP_024916550.1 type 4a pilus biogenesis protein PilN -
  PALA52_RS26020 (PALA52_05161) pilM 5647968..5649032 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 77504.07 Da        Isoelectric Point: 5.2241

>NTDB_id=754281 PALA52_RS26000 WP_024916548.1 5644030..5646174(-) (pilQ) [Pseudomonas aeruginosa strain PALA52]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASAAPVKASPAPASYAQPIKPKPYVPAG
RSIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSIII
EPSGLYDYLVYQTDNRLTVSIKPMTTEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SDGGQEGKEGGRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHKGNW
NGYGKDGNIGIKDEDGMNCGPIAGNCTFPTTGTSKSPSPFVDIGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEIVS
QPKVVTSDKETAKILKGQEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDYQNVMNGVPPINKN
EVNAKILVNDGETIVIGGVFSNEQTKSVDKVPFLGDLPYLGRLFRRDTVTDRKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=754281 PALA52_RS26000 WP_024916548.1 5644030..5646174(-) (pilQ) [Pseudomonas aeruginosa strain PALA52]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCATTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCACTGCCAGGGGACCGGGTCGAGCTCAAGCTGCAATTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTGCAGAACAAGCTCGGCACCAAGAACCGCGAA
CTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGACCGCACACGGTTGATCATCAACCTGACCACGCT
GTCGTCCTATACCACCCGGGTAGAAGGCAACAACCTCTTCGTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTT
CCGCCGCTCCGGTCAAGGCAAGCCCGGCTCCGGCCAGCTACGCCCAGCCGATCAAGCCCAAGCCATACGTGCCGGCGGGC
CGCTCCATCCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTT
GAGCCCGGATATCCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAACTGCCGGATGCGCTGCGGGTTC
GCCTGGATGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGTGCGCAATCCGACCGTACCAGCATCATCATC
GAACCGAGCGGGCTCTACGATTACCTGGTCTACCAGACTGACAATCGCCTGACCGTCAGCATCAAGCCGATGACCACGGA
AGACGCCGAGCGGCGCAAGAAGGACAACTTCGCCTATACCGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTATTGCAGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCGGATACGGTGCAGGGCAACATCACCCTG
CGCCTGCAGAACGTACCGTGGGACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTACTGCTGGTGGCACCGGCCGACGAGATCGCTGCCCGCGAGCGTCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGT
TGGCGCCGCTGCGCCGGGAGCTGATCCAGGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACC
AGCGATGGTGGCCAGGAAGGCAAGGAAGGCGGTCGTGGCTCAATCACCGTCGACGACCGTACCAACAGCATCATCGCCTA
TCAGCCCCAGGAGCGCCTGGACGAGCTGCGGCGGATCGTTTCGCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGG
CGCGCATCGTCGAGGCCAACGTCGGCTACGACAAGAGCCTCGGGGTACGCTGGGGTGGCGCTTACCACAAGGGCAACTGG
AACGGCTACGGCAAGGACGGCAATATCGGGATCAAGGACGAGGACGGCATGAACTGCGGTCCGATCGCCGGCAACTGTAC
TTTCCCCACCACGGGTACCAGCAAGTCGCCGTCGCCGTTCGTCGACATCGGCGCCAAGGACGCTACCTCGGGGATCGGCA
TCGGCTTCATCACCGACAACATCATTCTCGACCTGCAACTGTCGGCGATGGAAAAGACCGGCAACGGCGAGATCGTCTCC
CAGCCCAAGGTCGTGACCTCGGACAAGGAAACCGCGAAGATCCTCAAGGGCCAGGAAGTGCCCTACCAGGAAGCCAGTTC
CAGCGGCGCTACCAGCACCTCGTTCAAGGAGGCCGCGCTGTCCCTCGAGGTGACCCCGCAGATCACGCCGGACAACCGGA
TCATCGTCGAAGTGAAGGTGACCAAGGATGCGCCGGATTACCAAAACGTGATGAATGGTGTGCCTCCGATCAACAAGAAC
GAGGTCAACGCCAAGATCCTGGTCAACGATGGCGAGACCATCGTCATCGGCGGCGTATTCAGCAACGAGCAGACCAAGTC
GGTGGACAAGGTGCCGTTCCTCGGCGACCTGCCCTACCTCGGGCGGCTGTTCCGGCGCGATACCGTTACCGACAGGAAAA
ACGAACTTCTGGTTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(549-705)

AMIN domain (AMIN)

(38-122)

Secretin and TonB N terminus short domain (STN)

(307-353)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

98.319

100

0.983
  pilQ Acinetobacter baumannii strain A118

40.699

100

0.408
  pilQ Acinetobacter baumannii D1279779

40.699

100

0.408
  comQ Acinetobacter baylyi ADP1

41.11

98.459

0.405