Detailed information NTG0754253

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PALA52_RS02045
• Genome accession: NZ_CP110347
• Coordinates: 442416..443564 (+)
• Length: 382 a.a.
• NCBI ID: WP_263957462.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain PALA52
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 437416..448564

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PALA52_RS02020	-	438567..438863 (-)	297	WP_121367052.1	DUF167 domain-containing protein	-
PALA52_RS02025 (PALA52_00402)	-	438860..439453 (-)	594	WP_003155373.1	YggT family protein	-
PALA52_RS02030 (PALA52_00403)	proC	439464..440285 (-)	822	WP_124181617.1	pyrroline-5-carboxylate reductase	-
PALA52_RS02035 (PALA52_00404)	-	440297..440989 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PALA52_RS02040 (PALA52_00405)	pilT	441204..442238 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PALA52_RS02045 (PALA52_00406)	pilU	442416..443564 (+)	1149	WP_263957462.1	type IV pilus ATPase PilU	Machinery gene
PALA52_RS02050 (PALA52_00407)	czcD	443572..444471 (-)	900	WP_024918014.1	CDF family zinc transporter CzcD	-
PALA52_RS02055 (PALA52_00408)	-	444625..445029 (+)	405	WP_024918013.1	NINE protein	-
PALA52_RS02060 (PALA52_00409)	-	445272..446645 (+)	1374	WP_024918012.1	pyridoxal-phosphate dependent enzyme	-
PALA52_RS02065 (PALA52_00410)	-	446642..447826 (+)	1185	WP_024918011.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42522.77 Da
• Isoelectric Point: 6.7792

>NTDB_id=754253 PALA52_RS02045 WP_263957462.1 442416..443564(+) (pilU) [Pseudomonas aeruginosa strain PALA52]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMSVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=754253 PALA52_RS02045 WP_263957462.1 442416..443564(+) (pilU) [Pseudomonas aeruginosa strain PALA52]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGTCGGTGACCAAGACCCCGTTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGTCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	64.986	93.455	0.607
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382