Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OH720_RS01795 Genome accession   NZ_CP110128
Coordinates   394600..395661 (+) Length   353 a.a.
NCBI ID   WP_272604327.1    Uniprot ID   -
Organism   Pseudomonas sp. WJP1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 389600..400661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OH720_RS01780 (OH720_01780) rpmE 389932..390150 (+) 219 WP_008047849.1 50S ribosomal protein L31 -
  OH720_RS01785 (OH720_01785) - 390437..391705 (+) 1269 WP_272604325.1 malic enzyme-like NAD(P)-binding protein -
  OH720_RS01790 (OH720_01790) - 391945..394386 (-) 2442 WP_272604326.1 penicillin-binding protein 1A -
  OH720_RS01795 (OH720_01795) pilM 394600..395661 (+) 1062 WP_272604327.1 pilus assembly protein PilM Machinery gene
  OH720_RS01800 (OH720_01800) - 395661..396227 (+) 567 WP_272604328.1 PilN domain-containing protein -
  OH720_RS01805 (OH720_01805) pilO 396224..396844 (+) 621 WP_272604329.1 type 4a pilus biogenesis protein PilO -
  OH720_RS01810 (OH720_01810) - 396844..397368 (+) 525 WP_272604330.1 pilus assembly protein PilP -
  OH720_RS01815 (OH720_01815) pilQ 397382..399424 (+) 2043 WP_272604331.1 type IV pilus secretin PilQ Machinery gene
  OH720_RS01820 (OH720_01820) aroK 399429..399947 (+) 519 WP_032831512.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 37610.98 Da        Isoelectric Point: 4.7316

>NTDB_id=752526 OH720_RS01795 WP_272604327.1 394600..395661(+) (pilM) [Pseudomonas sp. WJP1]
MLGLFNKKARSLLGIDISSTSVKLLELSRQGNGYKVEAYAVEPLPFSAVVEKNIAEPDGVGQALSRALVKARTNLKSVAV
AVAGSAVITKTIEMDAGLTDDEMEDQLKIEADQYIPYPLDEVAIDFEVQGASARNPGRVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAHALERSFGLLESQQGGAQGRLVAVVDIGATMTTLSVLHDGRIIYTREQLFGGRQLTEEIGRRYGLTAE
QAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQQLGTPTQVANPF
CDMTLGIKVNAAALASDAPALMIACGLALRGFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=752526 OH720_RS01795 WP_272604327.1 394600..395661(+) (pilM) [Pseudomonas sp. WJP1]
GTGCTAGGACTCTTCAATAAAAAAGCCAGGTCGCTTCTGGGGATCGATATCAGCTCCACTTCGGTGAAGCTGCTGGAGCT
GAGCCGCCAGGGCAATGGCTATAAGGTCGAGGCCTACGCGGTCGAGCCATTGCCCTTCAGTGCGGTCGTCGAGAAAAATA
TCGCCGAACCCGATGGCGTGGGCCAGGCACTGTCGCGTGCGCTGGTCAAGGCCAGGACCAACCTCAAGAGCGTGGCGGTG
GCCGTGGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGATGCCGGCCTTACCGACGACGAGATGGAAGATCAGCT
CAAGATCGAGGCCGACCAGTACATCCCTTATCCGTTGGACGAGGTCGCCATCGATTTCGAAGTCCAGGGTGCGTCAGCGC
GCAATCCTGGTCGGGTCAATGTGCTGCTCGCCGCCTGTCGCAAGGAAAACGTCGAGGTCCGTGAGGCCGCCCTGGCGCTG
GCGGGCTTGACTGCGCGCGTGGTCGACGTTGAAGCCCATGCACTGGAGCGTTCTTTCGGTTTGCTCGAGAGTCAGCAGGG
TGGCGCTCAAGGGCGTCTGGTGGCAGTGGTCGATATCGGGGCCACGATGACCACCCTGAGCGTGCTGCATGACGGACGGA
TCATCTACACCCGCGAGCAATTGTTCGGTGGCCGCCAGCTCACGGAGGAAATTGGGCGCCGATATGGCCTGACCGCTGAG
CAGGCCGGCCTGGCGAAGAAGCAGGGCGGTTTGCCGGATGATTATGTCAGTGAAGTGTTGCAGCCATTTCGAGAGGCGCT
GGTGCAGCAAGTGTCGCGTTCATTGCAGTTCTTCTTCGCTTCGGGCCAATACAACGCGGTTGACCATATCCTGCTGGCAG
GCGGTACCGCGTCAGTTCCCGGCCTGGACCGGTTGATCGAGCAGCAATTGGGCACGCCAACCCAGGTGGCCAATCCGTTT
TGCGACATGACGCTGGGTATCAAGGTCAACGCGGCGGCCCTGGCCAGCGATGCACCGGCGTTGATGATCGCCTGCGGGCT
GGCCCTCAGGGGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.085

100

0.552
  comM Acinetobacter nosocomialis M2

55.085

100

0.552
  comM Acinetobacter baylyi ADP1

53.107

100

0.533
  pilM Legionella pneumophila strain ERS1305867

49.288

99.433

0.49