Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OK117_RS11165 Genome accession   NZ_CP109886
Coordinates   2408564..2410297 (+) Length   577 a.a.
NCBI ID   WP_058564300.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. fastidiosa strain CFBP8073     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2403564..2415297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OK117_RS11140 (OK117_11150) pilC 2403873..2405075 (-) 1203 WP_011098309.1 type II secretion system F family protein Machinery gene
  OK117_RS11145 (OK117_11155) - 2405516..2405962 (+) 447 WP_057682388.1 pilin -
  OK117_RS11150 (OK117_11160) - 2406210..2406437 (-) 228 WP_058564297.1 hypothetical protein -
  OK117_RS11155 (OK117_11165) - 2406837..2407136 (-) 300 WP_155561232.1 hypothetical protein -
  OK117_RS11160 (OK117_11170) - 2407280..2407708 (+) 429 WP_306308946.1 pilin -
  OK117_RS11165 (OK117_11175) pilB 2408564..2410297 (+) 1734 WP_058564300.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OK117_RS11170 (OK117_11180) pilR 2410391..2411803 (-) 1413 WP_058564301.1 sigma-54 dependent transcriptional regulator Regulator
  OK117_RS11175 (OK117_11185) - 2411989..2413599 (-) 1611 WP_004090372.1 ATP-binding protein -
  OK117_RS11180 (OK117_11190) sucC 2413854..2415017 (+) 1164 WP_004090371.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62955.99 Da        Isoelectric Point: 5.8793

>NTDB_id=750391 OK117_RS11165 WP_058564300.1 2408564..2410297(+) (pilB) [Xylella fastidiosa subsp. fastidiosa strain CFBP8073]
MNTPHSANLVGITGIARRLVQDGSIEEATARTAQNQAMAAKVPLPQWLIDKKLVSAAKLAAANALEFGMPLLDVSAFDHN
HNAMNLVDEELLQKHRVLPLFKRGNRLFVGISNPTQTQALDDIKFHTNLVVEPILVDEDQIRRTLEQWQSRSGALSSALD
EEDNGIEGLDIGGGDDDGASGDTGIDAKSDDTPVVKFVNKVLIDAIRRGASDIHFEPYEEDYRVRFRIDGLLKAVAKAPN
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQVDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQTL
FMEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
VIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKRPVQLPT
NALLAEGFTQEQIDAGLELYEPVGCDECTEGYKGRTGIYQVMPITEGISSIILAGGNALQIAEEAQHIGINDLRQSALLK
VAQGITGLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=750391 OK117_RS11165 WP_058564300.1 2408564..2410297(+) (pilB) [Xylella fastidiosa subsp. fastidiosa strain CFBP8073]
ATGAATACACCTCATTCAGCCAATCTTGTCGGCATCACCGGTATTGCCCGTCGCCTGGTCCAGGATGGCAGCATTGAGGA
AGCCACTGCACGCACTGCTCAAAATCAGGCAATGGCCGCAAAAGTGCCTTTGCCGCAGTGGTTGATCGATAAAAAACTAG
TGAGTGCAGCAAAATTGGCCGCGGCCAATGCACTTGAGTTCGGGATGCCGCTACTGGATGTGTCCGCATTCGACCACAAT
CACAATGCAATGAACTTGGTCGACGAAGAATTACTGCAAAAGCATCGGGTGTTGCCATTATTTAAACGCGGTAATCGACT
GTTCGTCGGGATCAGCAATCCAACTCAAACACAGGCCCTGGACGACATCAAATTCCACACCAATCTGGTCGTGGAACCGA
TTCTGGTCGACGAAGACCAGATACGGCGCACACTTGAACAATGGCAATCTAGGAGCGGCGCGCTCAGCTCCGCATTGGAT
GAAGAGGATAACGGCATTGAAGGGCTGGATATCGGCGGCGGAGACGACGACGGGGCCAGTGGCGATACCGGGATTGATGC
CAAGAGCGATGACACACCTGTCGTCAAATTCGTCAACAAAGTGCTGATTGATGCCATCCGACGCGGCGCATCAGACATCC
ACTTTGAACCATACGAAGAAGATTACCGCGTCCGTTTCCGGATTGATGGTCTGCTGAAGGCTGTGGCAAAAGCACCGAAC
AAGTTGAATCAGCGTATCGCCGCACGCTTGAAAGTCATGTCGCAACTGGATATCGCGGAAAAACGCGTCCCTCAAGACGG
ACGTATCAAGCTTAATCTGTCGAAAACCAAGCAAGTCGATTTCCGCGTCAGCACCTTGCCAACACTGTTCGGCGAGAAAG
TGGTGCTGCGTATCTTGGATGGCAGCGCGGCAAAGCTGGGTATCGACAAACTCGGTTACGAAGCTGACCAACAAACGCTT
TTTATGGAAGCAATCCACAAACCCTATGGAATGGTACTGGTCACTGGTCCGACCGGTTCGGGCAAGACAGTGTCACTGTA
CACTGCGTTAGGCATCCTCAATGACGAAACACGCAATATCTCCACAGCCGAAGATCCTGTAGAAATCCGGCTGCCGGGTG
TCAATCAGGTACAACAAAACAACAAGCGCGGTATGACGTTTGCCGCAGCATTACGTTCGTTTCTGCGCCAAGATCCGGAT
GTCATTATGGTCGGCGAAATCCGTGATCTGGAAACCGCTGAAATCGCCATCAAAGCCGCACAGACCGGCCACATGGTGCT
CTCCACCCTACATACCAACGATGCACCGCAAACCATCGCACGCCTCATGAACATGGGGATTGCACCATACAACATCACTT
CATCCGTGACCTTAGTGATTGCACAGCGATTGGCACGGAGATTGTGCAACAAGTGCAAGCGCCCGGTTCAATTGCCGACA
AACGCATTACTGGCCGAAGGATTCACTCAAGAACAAATTGATGCGGGGCTGGAATTATACGAGCCTGTCGGTTGCGACGA
ATGCACTGAAGGCTACAAAGGCCGCACAGGTATTTATCAAGTGATGCCAATTACCGAAGGGATTTCCTCGATCATTTTAG
CCGGCGGTAATGCCTTACAAATCGCCGAAGAGGCACAGCACATCGGCATCAACGACCTACGCCAATCGGCGCTACTCAAA
GTCGCTCAGGGTATCACCGGGCTGGCTGAAATTAATCGAGTCACCAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

56.966

98.267

0.56
  pilB Acinetobacter baumannii D1279779

56.614

98.267

0.556
  pilB Legionella pneumophila strain ERS1305867

52.456

98.787

0.518
  pilF Neisseria gonorrhoeae MS11

50.439

98.614

0.497
  pilB Vibrio cholerae strain A1552

49.632

94.281

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

47.08

97.92

0.461
  pilB Vibrio campbellii strain DS40M4

46.303

98.44

0.456
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.993

92.894

0.362