Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PALA25_RS23530 Genome accession   NZ_CP109834
Coordinates   5075146..5076849 (+) Length   567 a.a.
NCBI ID   WP_003116249.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA25     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5070146..5081849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA25_RS23510 (PALA25_04691) nadC 5072603..5073451 (+) 849 WP_016562338.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PALA25_RS23520 (PALA25_04693) - 5073616..5074314 (-) 699 WP_023122772.1 hypothetical protein -
  PALA25_RS23525 - 5074378..5074917 (-) 540 WP_003116248.1 pilin -
  PALA25_RS23530 (PALA25_04694) pilB 5075146..5076849 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PALA25_RS23535 (PALA25_04695) pilC 5076853..5078070 (+) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  PALA25_RS23540 (PALA25_04696) pilD 5078073..5078945 (+) 873 WP_016562340.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PALA25_RS23545 (PALA25_04697) coaE 5078942..5079553 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  PALA25_RS23550 (PALA25_04698) yacG 5079550..5079750 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PALA25_RS23555 (PALA25_04699) - 5079787..5079996 (-) 210 WP_003094660.1 hypothetical protein -
  PALA25_RS23560 (PALA25_04700) - 5080102..5080791 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  PALA25_RS23565 (PALA25_04701) - 5080788..5081258 (-) 471 WP_003094664.1 hypothetical protein -
  PALA25_RS23570 (PALA25_04702) - 5081255..5081680 (-) 426 WP_016562341.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62610.54 Da        Isoelectric Point: 5.5603

>NTDB_id=749809 PALA25_RS23530 WP_003116249.1 5075146..5076849(+) (pilB) [Pseudomonas aeruginosa strain PALA25]
MNDTIQLSGLARQLVLHELLDEKAAQQAQSQAQRNKLSLVTYLVQSKLVKGQALIELAADQFGIAYCDLNSLERDSLPKD
LISEKLVRQHRVVPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGIIIEKLFENATDSLAGLDDVDLE
GLDVGSGAGASQDDDSSAETDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPKETLLHEGFP
EDRIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=749809 PALA25_RS23530 WP_003116249.1 5075146..5076849(+) (pilB) [Pseudomonas aeruginosa strain PALA25]
ATGAACGACACAATTCAACTCAGCGGCTTGGCTCGCCAGCTCGTCCTGCATGAACTTCTCGATGAAAAGGCCGCCCAACA
GGCCCAATCGCAGGCCCAGCGCAACAAGTTGTCGCTGGTAACCTATCTGGTACAGAGCAAGCTAGTGAAAGGGCAGGCCT
TGATCGAACTGGCTGCGGATCAATTCGGTATCGCCTACTGCGACCTGAACAGCCTTGAGCGGGACAGTCTGCCCAAGGAT
CTGATCAGCGAGAAGCTGGTGCGCCAGCATCGAGTAGTTCCGTTGTGGCGACGTGGCAACAAGCTGTTCGTCGGCATCTC
CGATCCAGCCAACCACCAGGCGATTAACGATGTGCAGTTCAGCACAGGCCTGACTACTGAAGCCATCCTGGTCGAGGATG
ACAAGCTCGGCATCATCATCGAGAAACTATTCGAAAATGCCACTGACAGCCTTGCAGGACTGGACGACGTGGATCTGGAA
GGCCTGGATGTCGGTTCCGGTGCGGGCGCAAGCCAGGATGATGATTCCAGTGCAGAGACAGACGACGCCCCGGTGGTGCG
TTTCGTCAACAAAATGTTGTTGGATGCGATCAGAGGCGGCTCCTCCGACCTGCATTTCGAGCCCTATGAAAAGATTTACC
GCGTACGTTTCCGCACCGATGGCATGCTCCATGAAGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGA
CTCAAGGTGATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGTATCCTCGATTCCTCCA
GCGCGCAGATGGGCATCGACGCCCTCGGCTACGAGGAAGACCAGAAGGAACTCTACCTGGCTGCGCTCAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCAC
CGACATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACTTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGAC
CTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTGACCCGCCTGCTGAACATGGGCGTGCCAGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGC
GACTTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAGCATGAGGTACCGAAGGAAACACTCCTCCATGAAGGCTTCCCC
GAGGATCGGATCGGCACCTTCAAGCTGTATTCACCAGTAGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.895

100

0.582
  pilB Acinetobacter baylyi ADP1

57.27

99.471

0.57
  pilB Legionella pneumophila strain ERS1305867

55.106

100

0.552
  pilF Neisseria gonorrhoeae MS11

50.444

99.295

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

51.948

95.062

0.494
  pilB Vibrio cholerae strain A1552

50.182

97.002

0.487
  pilB Vibrio campbellii strain DS40M4

50.832

95.414

0.485
  pilF Thermus thermophilus HB27

38.584

99.647

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.189

93.474

0.376