Detailed information NTG0749256

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: L0P93_RS14360
• Genome accession: NZ_CP109795
• Coordinates: 2824505..2825119 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain B19
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2819505..2830119

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L0P93_RS14330 (L0P93_14330)	rpsT	2819808..2820074 (+)	267	WP_277160817.1	30S ribosomal protein S20	-
L0P93_RS14335 (L0P93_14335)	holA	2820037..2821132 (-)	1096	Protein_2769	DNA polymerase III subunit delta	-
L0P93_RS14340 (L0P93_14340)	-	2821172..2821323 (-)	152	Protein_2770	hypothetical protein	-
L0P93_RS14345 (L0P93_14345)	-	2821364..2821498 (+)	135	WP_003152870.1	YqzM family protein	-
L0P93_RS14350 (L0P93_14350)	comEC	2821517..2823868 (-)	2352	WP_277160819.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
L0P93_RS14355 (L0P93_14355)	-	2823869..2824438 (-)	570	WP_003152868.1	ComE operon protein 2	-
L0P93_RS14360 (L0P93_14360)	comEA	2824505..2825119 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
L0P93_RS14365 (L0P93_14365)	comER	2825178..2825999 (+)	822	WP_029973401.1	late competence protein ComER	-
L0P93_RS14370 (L0P93_14370)	-	2826068..2826805 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
L0P93_RS14375 (L0P93_14375)	rsfS	2826802..2827158 (-)	357	WP_277160820.1	ribosome silencing factor	-
L0P93_RS14380 (L0P93_14380)	yqeK	2827176..2827736 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
L0P93_RS14385 (L0P93_14385)	-	2827726..2828295 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
L0P93_RS14390 (L0P93_14390)	yhbY	2828306..2828596 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
L0P93_RS14395 (L0P93_14395)	aroE	2828590..2829432 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=749256 L0P93_RS14360 WP_014418419.1 2824505..2825119(-) (comEA) [Bacillus velezensis strain B19]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=749256 L0P93_RS14360 WP_014418419.1 2824505..2825119(-) (comEA) [Bacillus velezensis strain B19]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363