NTDB

Overview

Name	comFC	Type	Machinery gene
Locus tag	N8A73_RS16240	Genome accession	NZ_CP109651
Coordinates	3143399..3144055 (-)	Length	218 a.a.
NCBI ID	WP_227001684.1	Uniprot ID	-
Organism	Bacillus safensis strain LG01
Function	ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3138399..3149055

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N8A73_RS16205	fliW	3138537..3138971 (-)	435	WP_024423615.1	flagellar assembly protein FliW	-
N8A73_RS16210	-	3139000..3139578 (-)	579	WP_024426253.1	DUF6470 family protein	-
N8A73_RS16215	flgL	3139625..3140533 (-)	909	WP_034281095.1	flagellar hook-associated protein FlgL	-
N8A73_RS16220	flgK	3140543..3142063 (-)	1521	WP_024423618.1	flagellar hook-associated protein FlgK	-
N8A73_RS16225	-	3142079..3142561 (-)	483	WP_024423619.1	flagellar protein FlgN	-
N8A73_RS16230	flgM	3142580..3142843 (-)	264	WP_024423620.1	flagellar biosynthesis anti-sigma factor FlgM	-
N8A73_RS16235	-	3142923..3143342 (-)	420	WP_034281098.1	TIGR03826 family flagellar region protein	-
N8A73_RS16240	comFC	3143399..3144055 (-)	657	WP_227001684.1	ComF family protein	Machinery gene
N8A73_RS16245	-	3144082..3144375 (-)	294	WP_024423623.1	late competence development ComFB family protein	-
N8A73_RS16250	comFA	3144424..3145821 (-)	1398	WP_254001458.1	DEAD/DEAH box helicase	Machinery gene
N8A73_RS16255	-	3145942..3146784 (-)	843	WP_024423625.1	DegV family protein	-
N8A73_RS16260	degU	3147000..3147689 (-)	690	WP_008348273.1	two-component system response regulator DegU	Regulator
N8A73_RS16265	degS	3147754..3148929 (-)	1176	WP_003213166.1	sensor histidine kinase	Regulator

Sequence

Protein

Download Length: 218 a.a. Molecular weight: 24868.96 Da Isoelectric Point: 9.9287

                                >NTDB_id=748764 N8A73_RS16240 WP_227001684.1 3143399..3144055(-) (comFC) [Bacillus safensis strain LG01]

MPAFTWKSFLLLNDDRLCVACKNHLVKIERPICPTCGRAQSNDQLCQDCNSWNERSDSIKVLVKNRSVYTYSELMKDVLA

RFKFRGDTALAELFKRDVQAAFKHSFCIKDPLLIPIPLSHERLKERGFNQSVIIASLLDKPILQPLVKIHQSKQSKKKKN

ERLDQKGLFQIKQTDAVVQRDIILVDDIYTTGATIYDAARILKDAGAKSVSSFTLIRS

Nucleotide

Download Length: 657 bp

                                >NTDB_id=748764 N8A73_RS16240 WP_227001684.1 3143399..3144055(-) (comFC) [Bacillus safensis strain LG01]

GTGCCTGCTTTTACATGGAAATCATTCCTACTATTAAATGATGATCGTCTTTGTGTAGCATGTAAGAATCACTTAGTAAA

GATTGAACGTCCTATTTGTCCAACATGCGGAAGGGCGCAGTCAAATGACCAATTATGCCAGGACTGTAATAGTTGGAATG

AACGCTCTGATTCTATTAAAGTCCTTGTAAAAAATAGGTCAGTTTATACTTACAGCGAGTTGATGAAAGATGTCCTTGCC

CGCTTTAAATTTCGGGGTGATACGGCTTTAGCTGAGCTGTTTAAAAGAGATGTACAAGCAGCATTTAAACATTCCTTCTG

CATAAAAGACCCATTGCTGATACCGATCCCTTTAAGTCATGAAAGACTAAAAGAAAGAGGATTTAATCAGTCCGTGATAA

TCGCTTCTTTGCTAGATAAGCCAATATTGCAGCCACTCGTGAAAATACATCAAAGTAAGCAGTCTAAAAAAAAGAAAAAT

GAACGCCTAGATCAAAAAGGATTGTTTCAAATCAAGCAAACAGATGCGGTTGTTCAAAGGGATATTATATTAGTTGATGA

TATCTATACAACGGGAGCAACCATCTATGATGCGGCAAGAATTTTGAAAGACGCAGGTGCTAAAAGTGTTTCTTCTTTTA

CGTTGATACGTAGTTAA

Domains

Predicted by InterproScan.

Phosphoribosyl transferase domain (Pribosyltran)

(127-216)

Double zinc ribbon domain (DZR_2)

(14-49)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comFC	Bacillus subtilis subsp. subtilis str. 168	49.537	99.083	0.491
comFC/cflB	Streptococcus pneumoniae Rx1	36.744	98.624	0.362
comFC/cflB	Streptococcus pneumoniae D39	36.744	98.624	0.362
comFC/cflB	Streptococcus pneumoniae R6	36.744	98.624	0.362
comFC/cflB	Streptococcus pneumoniae TIGR4	36.744	98.624	0.362

Detailed information NTG0748764