Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGC   Type   Machinery gene
Locus tag   N8A73_RS11265 Genome accession   NZ_CP109651
Coordinates   2203615..2203908 (-) Length   97 a.a.
NCBI ID   WP_024422977.1    Uniprot ID   A0A0M2EIN6
Organism   Bacillus safensis strain LG01     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2198615..2208908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8A73_RS11215 sinR 2198779..2199114 (+) 336 WP_024422969.1 transcriptional regulator SinR Regulator
  N8A73_RS11220 tasA 2199182..2199982 (-) 801 WP_176966992.1 biofilm matrix protein TasA -
  N8A73_RS11225 sipW 2200040..2200612 (-) 573 WP_034280218.1 signal peptidase I SipW -
  N8A73_RS11230 tapA 2200665..2201192 (-) 528 WP_041117967.1 amyloid fiber anchoring/assembly protein TapA -
  N8A73_RS11235 - 2201492..2201770 (+) 279 WP_061109527.1 DUF3889 domain-containing protein -
  N8A73_RS11240 - 2201805..2201999 (-) 195 WP_024422974.1 YqzE family protein -
  N8A73_RS11245 - 2202059..2202442 (-) 384 WP_041117969.1 competence type IV pilus minor pilin ComGG -
  N8A73_RS11250 comGF 2202439..2202966 (-) 528 WP_264315971.1 competence type IV pilus minor pilin ComGF -
  N8A73_RS11255 comGE 2202878..2203192 (-) 315 WP_264315972.1 competence type IV pilus minor pilin ComGE -
  N8A73_RS11260 comGD 2203176..2203622 (-) 447 WP_176966991.1 competence type IV pilus minor pilin ComGD -
  N8A73_RS11265 comGC 2203615..2203908 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  N8A73_RS11270 comGB 2203925..2204965 (-) 1041 WP_034280202.1 competence type IV pilus assembly protein ComGB -
  N8A73_RS11275 comGA 2204946..2206016 (-) 1071 WP_041117979.1 competence type IV pilus ATPase ComGA Machinery gene
  N8A73_RS11280 - 2206194..2207069 (-) 876 WP_024422979.1 STAS domain-containing protein -
  N8A73_RS11290 - 2207293..2207673 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  N8A73_RS11295 - 2207886..2208128 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  N8A73_RS11300 - 2208173..2208811 (-) 639 WP_176966990.1 MBL fold metallo-hydrolase -

Sequence


Protein


Download         Length: 97 a.a.        Molecular weight: 10686.62 Da        Isoelectric Point: 7.3218

>NTDB_id=748747 N8A73_RS11265 WP_024422977.1 2203615..2203908(-) (comGC) [Bacillus safensis strain LG01]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQAIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIVIQNGNVKHE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=748747 N8A73_RS11265 WP_024422977.1 2203615..2203908(-) (comGC) [Bacillus safensis strain LG01]
ATGAATGATAAAGGATTTACCCTGTTAGAAATGTTAATAGTCATGCTTGTAATATCCATTCTTTTACTCATTACCATACC
GAATATTACACGTCATCACCAAGCTATTCAGGCAAAGGGCTGTGAAGGGCTTAAATCAATGGTCAGCACACAAGTGATGG
CATATGAATTGGAGCATGATGGGAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGTTATGTCAAAAAAGGATTAACT
TGTCCTAATGGAAAAGCGATTGTCATTCAAAACGGCAACGTGAAGCATGAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0M2EIN6

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67

  comGC Staphylococcus aureus MW2

42.222

92.784

0.392

  comGC Staphylococcus aureus N315

42.222

92.784

0.392