Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OEG79_RS18580 Genome accession   NZ_CP107549
Coordinates   3916528..3917592 (-) Length   354 a.a.
NCBI ID   WP_264146417.1    Uniprot ID   -
Organism   Pseudomonas sp. Z8(2022)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3911528..3922592
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OEG79_RS18555 (OEG79_18555) aroK 3912115..3912633 (-) 519 WP_264146412.1 shikimate kinase AroK -
  OEG79_RS18560 (OEG79_18560) pilQ 3912639..3914759 (-) 2121 WP_264146413.1 type IV pilus secretin PilQ Machinery gene
  OEG79_RS18565 (OEG79_18565) pilP 3914815..3915339 (-) 525 WP_264146414.1 type 4a pilus biogenesis lipoprotein PilP -
  OEG79_RS18570 (OEG79_18570) pilO 3915339..3915959 (-) 621 WP_264146415.1 type 4a pilus biogenesis protein PilO -
  OEG79_RS18575 (OEG79_18575) pilN 3915956..3916528 (-) 573 WP_264146416.1 type 4a pilus biogenesis protein PilN -
  OEG79_RS18580 (OEG79_18580) pilM 3916528..3917592 (-) 1065 WP_264146417.1 pilus assembly protein PilM Machinery gene
  OEG79_RS18585 (OEG79_18585) - 3917777..3920185 (+) 2409 WP_413247556.1 penicillin-binding protein 1A -
  OEG79_RS18590 (OEG79_18590) - 3920288..3921556 (-) 1269 WP_264146419.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37813.20 Da        Isoelectric Point: 4.4647

>NTDB_id=740593 OEG79_RS18580 WP_264146417.1 3916528..3917592(-) (pilM) [Pseudomonas sp. Z8(2022)]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLAKAKSGVKAAAV
AVAGSAVITKTIEMEAGLSEDDLENQLKIEADQYIPYPLEEVAIDFEVQGPAPRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYSLVEAQLGGAHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=740593 OEG79_RS18580 WP_264146417.1 3916528..3917592(-) (pilM) [Pseudomonas sp. Z8(2022)]
GTGCTAGGGCTCTTCACTAAGAAAGCGAATACGCTGCTGGGGATCGATATCAGCTCGACTTCGGTCAAGCTCCTCGAATT
GAGTCGCTCGGGAAGCCGCTACAAGGTAGAGGCTTACGCTGTCGAGCCGCTTCCTCCAAATGCTGTGGTTGAAAAGAACA
TTGCCGAGCTGGAGGGGGTCGGTCAGGCGCTTTCCCGCGTTTTGGCCAAGGCCAAGAGTGGCGTCAAGGCAGCGGCTGTC
GCCGTTGCCGGTTCGGCTGTCATCACCAAGACCATCGAGATGGAAGCCGGGCTTTCCGAGGATGATCTGGAAAATCAGCT
GAAGATCGAGGCTGACCAGTACATTCCCTATCCGCTGGAAGAAGTCGCCATCGATTTTGAAGTTCAGGGTCCGGCTCCGC
GCAACCCCGAGCGCGTCGAGGTGCTTCTGGCCGCATGCCGCAAGGAGAACGTCGAAGTCCGTGAGGCTGCGCTGGCGCTC
GCCGGCCTGACCGCCAAAGTGGTGGACGTCGAGGCGTACGCTCTGGAGCGTGCCTACAGTCTGGTGGAGGCGCAGCTGGG
CGGAGCGCATGATGAGTTGACGGTCGCAGTGGTCGATATCGGCGCCACCATGACCACGCTCAGTGTTCTGCACAATGGTC
GCACCATCTATACCCGTGAACAGCTCTTCGGTGGCAAGCAGCTGACTGAGGAAATTCAGCGGCGCTATGGACTGTCTGTC
GAGGAAGCTGGCCTTGCCAAGAAGCAAGGTGGTCTTCCAGATGACTACGACAGCGAGGTGTTGCAACCGTTCAAAGAGGC
TGTCGTTCAACAGGTTTCCCGTTCGCTGCAGTTCTTCTTCGCCGCCGGGCAGTTCAACGATGTCGACTACATCCTTCTGG
CTGGCGGTACTGCGTCCATTCCCGATCTCGACCGGCTGATTCAGCAGAAGATCGGTACGCAGACGCTGGTGGCCAATCCC
TTTGCCGATATGGCGCTCAGCAGCAAGGTCAACGCCGGGGCGCTGGCCAGTGATGCGCCGGCACTGATGATTGCCTGTGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.901

100

0.571
  comM Acinetobacter nosocomialis M2

56.901

100

0.571
  comM Acinetobacter baylyi ADP1

53.39

100

0.534
  pilM Legionella pneumophila strain ERS1305867

44.915

100

0.449