Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OE648_RS02920 Genome accession   NZ_CP107544
Coordinates   608776..610476 (-) Length   566 a.a.
NCBI ID   WP_042610478.1    Uniprot ID   -
Organism   Pseudomonas moraviensis strain EFBE32     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 603776..615476
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OE648_RS02885 (OE648_02875) - 603786..604313 (-) 528 WP_064364122.1 MOSC domain-containing protein -
  OE648_RS02890 (OE648_02880) - 604310..604936 (-) 627 WP_007909887.1 DUF1780 domain-containing protein -
  OE648_RS02895 (OE648_02885) - 605126..605815 (+) 690 WP_065615664.1 energy-coupling factor ABC transporter permease -
  OE648_RS02900 (OE648_02890) yacG 605865..606065 (-) 201 WP_042610482.1 DNA gyrase inhibitor YacG -
  OE648_RS02905 (OE648_02895) coaE 606062..606685 (-) 624 WP_042610481.1 dephospho-CoA kinase -
  OE648_RS02910 (OE648_02900) pilD 606682..607554 (-) 873 WP_065615666.1 prepilin peptidase Machinery gene
  OE648_RS02915 (OE648_02905) pilC 607556..608773 (-) 1218 WP_065615667.1 type II secretion system F family protein Machinery gene
  OE648_RS02920 (OE648_02910) pilB 608776..610476 (-) 1701 WP_042610478.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OE648_RS02925 (OE648_02915) - 610703..611140 (+) 438 WP_064364126.1 pilin -
  OE648_RS02930 (OE648_02920) - 611441..611794 (+) 354 WP_064364127.1 BON domain-containing protein -
  OE648_RS02935 (OE648_02925) - 612062..612361 (-) 300 WP_064364128.1 DUF2845 domain-containing protein -
  OE648_RS02940 (OE648_02930) - 612358..615309 (-) 2952 WP_333715332.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62597.63 Da        Isoelectric Point: 6.0450

>NTDB_id=740519 OE648_RS02920 WP_042610478.1 608776..610476(-) (pilB) [Pseudomonas moraviensis strain EFBE32]
MNDIALSGLAKQLVQAELLTETSARQSWAQAQRNRVSLVNYLVHNKLVNSRQIAEIASEHFGMALLDLNCLDKETQPKGL
VSEKLVRQHCALPLWRRGNKLFVGLSDPSNQQAISDIQFSTGLSTEAILVEDDKLSDAIDKFFDSHASGLEEMADVDLDG
LDVESIDDNKQDVIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRMRTDGMLREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEAEQKALYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCAHCKRPLEIPHETLLKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRRDGFADLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=740519 OE648_RS02920 WP_042610478.1 608776..610476(-) (pilB) [Pseudomonas moraviensis strain EFBE32]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTCCAGGCCGAATTGCTCACGGAAACAAGCGCCCGGCAATC
TTGGGCGCAGGCCCAGCGCAATCGTGTGTCGCTGGTCAATTACCTGGTGCACAACAAACTGGTCAACAGCCGGCAGATCG
CCGAGATCGCCTCGGAACATTTCGGCATGGCGCTGCTCGATCTCAACTGCCTCGACAAAGAAACCCAGCCCAAGGGCCTG
GTCAGTGAAAAACTGGTTCGCCAGCACTGCGCCCTGCCCTTGTGGCGACGTGGCAACAAATTGTTCGTGGGTCTATCCGA
CCCGAGCAATCAGCAGGCGATCAGCGACATCCAGTTCAGCACCGGCCTGAGCACCGAAGCGATTCTGGTCGAGGACGACA
AGCTCAGCGACGCCATCGACAAGTTCTTCGACAGCCATGCTTCGGGCCTGGAAGAAATGGCCGATGTCGATCTCGACGGC
CTCGACGTCGAGTCCATCGATGACAACAAGCAGGACGTTATCGGCGGCCTCGATGCGGACGATGCTCCAGTCGTGCGCTT
CGTGCACAAGATGCTGCTCGATGCGATCAAGAGCGGCTCTTCCGACCTGCATTTCGAGCCCTACGAGAAAAACTACCGGG
TACGCATGCGCACCGACGGCATGCTGCGCGAGGTGGCCAAGCCGCCGATCCAGTTGGCCGGGCGCATTGCCGCACGTTTG
AAGGTCATGGCCAGCCTCGATATTTCGGAACGGCGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCGAAAAGCAA
ATCCATCGATTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGTG
CGCAAATGGGCATCGACGCCTTGGGTTACGAGGCCGAACAAAAAGCCTTGTATCTCGCTGCGCTGAAACAGCCGCAAGGG
ATGATTCTGGTCACTGGCCCCACGGGCTCCGGTAAAACCGTGTCGCTGTACACCGGGCTGAACATCCTCAACACCGTCGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCCGCCAGG
GGCTGGATTTCGCTCAGGCCCTGCGTTCGTTTCTGCGTCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCGATCAAGGCTGCGCAGACCGGTCACCTGGTGCTGTCGACCCTGCACACCAACAGCGCGGCGGA
AACCCTCACGCGCCTGCACAACATGGGCATTCCCGGTTTCAACATCGCCACCTCGGTGAGCCTGATCATCGCCCAGCGTC
TGGCGCGCAAATTGTGCGCGCACTGCAAGCGGCCGCTGGAGATCCCGCACGAAACCTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGTCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAATGGCGGTTACAAAGGACGCGTGGGGAT
TTATGAAGTGGTAAAAAACACACCGGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCGCTGGAAATCGACATCCAGA
TGCGCCGCGACGGTTTCGCCGACCTGCGCACCTCCGGGCTGCACAAGGCCATGCAAGGCATCACCAGCCTTGAGGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.699

99.823

0.576
  pilB Acinetobacter baumannii D1279779

57.447

99.647

0.572
  pilB Legionella pneumophila strain ERS1305867

56.714

100

0.567
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50.446

99.117

0.5
  pilF Neisseria gonorrhoeae MS11

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.385

100

0.496
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.065

94.523

0.369