Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OCT39_RS08635 Genome accession   NZ_CP107007
Coordinates   1855068..1856813 (-) Length   581 a.a.
NCBI ID   WP_263587240.1    Uniprot ID   -
Organism   Halomonas sp. GD1P12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1850068..1861813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCT39_RS08605 (OCT39_08605) - 1850750..1851100 (+) 351 WP_311960807.1 hypothetical protein -
  OCT39_RS08610 (OCT39_08610) - 1851072..1851944 (-) 873 WP_263587235.1 DNA replication terminus site-binding protein -
  OCT39_RS08615 (OCT39_08615) yacG 1851990..1852220 (-) 231 WP_263587236.1 DNA gyrase inhibitor YacG -
  OCT39_RS08620 (OCT39_08620) coaE 1852279..1852905 (-) 627 WP_263587237.1 dephospho-CoA kinase -
  OCT39_RS08625 (OCT39_08625) - 1852934..1853779 (-) 846 WP_263587238.1 prepilin peptidase -
  OCT39_RS08630 (OCT39_08630) - 1853833..1855059 (-) 1227 WP_263587239.1 type II secretion system F family protein -
  OCT39_RS08635 (OCT39_08635) pilB 1855068..1856813 (-) 1746 WP_263587240.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OCT39_RS17415 - 1857035..1857457 (+) 423 WP_318152982.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OCT39_RS08645 (OCT39_08645) - 1857909..1858361 (+) 453 WP_263587241.1 anti-virulence regulator CigR family protein -
  OCT39_RS08650 (OCT39_08650) - 1858427..1859230 (+) 804 WP_263587242.1 inositol monophosphatase family protein -
  OCT39_RS08660 (OCT39_08660) - 1859466..1859645 (-) 180 WP_263587243.1 hypothetical protein -
  OCT39_RS08665 (OCT39_08665) - 1859739..1859942 (-) 204 WP_263587244.1 hypothetical protein -
  OCT39_RS08670 (OCT39_08670) - 1860040..1860765 (-) 726 WP_263587245.1 sulfite exporter TauE/SafE family protein -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63678.06 Da        Isoelectric Point: 5.4549

>NTDB_id=737296 OCT39_RS08635 WP_263587240.1 1855068..1856813(-) (pilB) [Halomonas sp. GD1P12]
MSSSAFDQPLASRALSGIAKRLVAHEVMTQGQAEVADTTSAELEVSFLEHVVQSGLVGSVDAALAAAWEYGLPVVDLEAI
KISALPPASDYPVKILEKLNVLPLARHGHRLTVAVPYPATLTRLDELQFATSLSVEGVLAPVEQIRNNLAQYVSQNERSL
LDELEGVDDAVGELNVVQQEEEEEVTLESSVTNKDDAPVVKFVNKVLLDAIGRGASDIHFEPFESQYRVRFRIDGMLIEV
AKPPFSMRDRIAARLKIMSRLDISERRLPQDGAIKLQLSRTRSIDFRVNSLPTVYGEKLVLRILDPSSVRLGIEQLGFSP
TQQREYEYALKRPQGMILVTGPTGSGKTVTLYTGINMLNEIERNICTAEDPVEIKVSGINQVNVLPKIGLNFASALRAFL
RQDPDVVMVGEIRDLETAEIAVKAAQTGHMVLSTVHTNSAAQTLTRLANMGVASYNIASSVTLIIAQRLARRLCPSCKAP
ADIPQEALLKAGFRKEQLTNVTIYKAVGCKQCTQGYKGRVGIYEVLPISDAMRNLIMRDGNALEIDELARKEGHPSLYQS
CLARVLDGSTSLEEVNRISKE

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=737296 OCT39_RS08635 WP_263587240.1 1855068..1856813(-) (pilB) [Halomonas sp. GD1P12]
ATGTCCTCATCCGCCTTCGATCAGCCCTTGGCTTCACGCGCGCTTAGCGGTATCGCGAAACGGCTCGTCGCTCATGAGGT
GATGACCCAGGGCCAGGCCGAAGTCGCCGATACGACGTCTGCCGAGCTCGAAGTCTCGTTTTTGGAGCACGTAGTACAAA
GCGGGCTGGTAGGCTCGGTCGATGCAGCGCTCGCGGCGGCCTGGGAGTACGGTTTGCCGGTAGTGGATCTGGAGGCGATC
AAGATCAGCGCGCTTCCTCCGGCCAGCGACTATCCGGTCAAGATTCTCGAAAAGCTGAACGTTTTGCCGCTGGCACGCCA
CGGCCACCGGCTGACCGTGGCAGTCCCCTACCCGGCCACGCTCACACGGCTCGACGAGCTGCAGTTTGCGACCAGCCTGA
GTGTCGAGGGCGTGCTGGCGCCGGTCGAGCAGATACGCAATAACCTGGCGCAGTACGTCTCGCAAAACGAGCGTAGCCTG
CTGGATGAGTTGGAAGGCGTGGACGACGCCGTGGGCGAGCTCAATGTCGTGCAGCAGGAAGAGGAGGAAGAGGTTACGCT
CGAGTCCTCGGTGACGAACAAGGATGACGCACCGGTCGTGAAGTTCGTCAATAAGGTGCTGCTGGACGCCATCGGCCGCG
GGGCGTCGGATATTCACTTCGAGCCGTTCGAATCCCAGTACCGGGTTCGCTTTCGTATCGACGGCATGCTCATCGAGGTC
GCTAAACCGCCCTTTTCGATGCGCGACCGGATCGCCGCACGCCTGAAGATCATGTCGCGACTCGATATCTCCGAGCGTCG
CCTGCCCCAGGACGGCGCGATCAAGCTTCAGCTCTCGCGCACCCGCTCGATCGACTTTCGCGTCAACTCCCTGCCCACCG
TGTATGGCGAAAAGCTGGTGCTGCGGATACTCGACCCCTCCTCGGTGCGACTTGGCATCGAGCAACTGGGCTTTAGCCCC
ACACAGCAGCGCGAGTACGAATACGCGCTCAAGCGCCCCCAGGGCATGATTCTGGTCACCGGGCCAACCGGTAGCGGCAA
GACCGTGACGCTTTATACCGGCATCAACATGCTCAATGAGATCGAGCGCAATATTTGTACCGCTGAGGATCCGGTCGAGA
TCAAGGTGTCCGGCATCAACCAGGTCAACGTGCTGCCCAAAATAGGGCTCAACTTTGCCAGCGCTCTAAGGGCGTTTTTG
CGCCAAGATCCGGACGTGGTCATGGTCGGCGAGATTCGCGACCTCGAAACCGCCGAGATCGCCGTCAAGGCGGCGCAAAC
CGGCCACATGGTGCTCTCCACGGTTCACACCAATTCGGCGGCGCAAACCCTGACGCGCCTGGCCAATATGGGGGTGGCCT
CTTATAACATCGCAAGTTCAGTCACGCTGATTATTGCTCAGCGCCTTGCGCGCCGGCTTTGCCCGAGCTGCAAAGCGCCG
GCGGACATTCCTCAGGAAGCGCTTTTAAAAGCCGGCTTTCGCAAGGAGCAGCTGACCAACGTGACGATCTATAAAGCGGT
TGGCTGTAAACAGTGCACTCAGGGGTACAAGGGCCGGGTCGGTATTTATGAAGTGCTGCCGATCAGCGATGCCATGCGCA
ACCTGATCATGCGTGACGGCAACGCGCTCGAAATCGACGAGCTGGCGCGCAAGGAGGGCCATCCGAGCCTTTACCAAAGC
TGTCTTGCGCGCGTGCTCGATGGCAGCACGAGCCTTGAAGAAGTCAACCGTATCAGCAAGGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-156)

Type II/IV secretion system protein (T2SSE)

(205-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

51.323

97.59

0.501
  pilB Acinetobacter baumannii D1279779

49.825

98.451

0.491
  pilB Acinetobacter baylyi ADP1

48.021

100

0.48
  pilB Vibrio cholerae strain A1552

51.367

88.124

0.453
  pilB Vibrio parahaemolyticus RIMD 2210633

48.513

92.599

0.449
  pilB Vibrio campbellii strain DS40M4

48.148

92.943

0.448
  pilF Neisseria gonorrhoeae MS11

45.39

97.074

0.441
  pilB/pilB1 Synechocystis sp. PCC 6803

38.364

94.664

0.363