Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P7249_RS04155 Genome accession   NZ_CP120944
Coordinates   833377..834357 (+) Length   326 a.a.
NCBI ID   WP_001055622.1    Uniprot ID   -
Organism   Escherichia coli O157:H7 str. EDL933     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 828377..839357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7249_RS04125 yggM 828804..829811 (+) 1008 WP_000745248.1 DUF1202 family protein -
  P7249_RS04130 hemW 830054..831190 (-) 1137 WP_000239959.1 radical SAM family heme chaperone HemW -
  P7249_RS04135 rdgB 831183..831776 (-) 594 WP_001174762.1 XTP/dITP diphosphatase -
  P7249_RS04140 yggU 831784..832074 (-) 291 WP_001277224.1 DUF167 family protein YggU -
  P7249_RS04145 yggT 832071..832637 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  P7249_RS04150 yggS 832655..833359 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  P7249_RS04155 pilT 833377..834357 (+) 981 WP_001055622.1 type IV pilus twitching motility protein PilT Machinery gene
  P7249_RS04160 ruvX 834548..834964 (-) 417 WP_000017110.1 Holliday junction resolvase RuvX -
  P7249_RS04165 yqgE 834964..835527 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  P7249_RS04170 gshB 835639..836586 (-) 948 WP_000593272.1 glutathione synthase -
  P7249_RS04175 rsmE 836599..837330 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  P7249_RS04180 endA 837410..838117 (-) 708 WP_000286494.1 deoxyribonuclease I -
  P7249_RS04185 yggI 838212..838709 (-) 498 WP_001303653.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35982.18 Da        Isoelectric Point: 5.7654

>NTDB_id=735342 P7249_RS04155 WP_001055622.1 833377..834357(+) (pilT) [Escherichia coli O157:H7 str. EDL933]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLTENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGTPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=735342 P7249_RS04155 WP_001055622.1 833377..834357(+) (pilT) [Escherichia coli O157:H7 str. EDL933]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGACGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CACACCACCGATATTGCCGGAGTTACTCAAGAGCGAGAATGGCTTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGTTATCTCAATCAACATGCTGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATCGGTTTGCATTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTTGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACTGGGCAACAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(61-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

37.654

99.387

0.374