Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N8888_RS14750 Genome accession   NZ_CP106759
Coordinates   3215463..3217190 (-) Length   575 a.a.
NCBI ID   WP_053517028.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain SG.Y2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3210463..3222190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8888_RS14735 (N8888_14735) sucC 3210846..3212015 (-) 1170 WP_005418614.1 ADP-forming succinate--CoA ligase subunit beta -
  N8888_RS14740 (N8888_14740) - 3212346..3213965 (+) 1620 WP_053517024.1 ATP-binding protein -
  N8888_RS14745 (N8888_14745) pilR 3214020..3215393 (+) 1374 WP_053517026.1 sigma-54 dependent transcriptional regulator Regulator
  N8888_RS14750 (N8888_14750) pilB 3215463..3217190 (-) 1728 WP_053517028.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N8888_RS14755 (N8888_14755) - 3217202..3220249 (-) 3048 WP_263175468.1 fused MFS/spermidine synthase -
  N8888_RS14760 (N8888_14760) - 3220428..3220874 (-) 447 WP_053517032.1 pilin -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62221.22 Da        Isoelectric Point: 6.0032

>NTDB_id=734582 N8888_RS14750 WP_053517028.1 3215463..3217190(-) (pilB) [Stenotrophomonas maltophilia strain SG.Y2]
MSVVSSPNLVGITGLARRLVQDGALDEATARDAMTRAAAARQPLPQWFAQNKVVGAAQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHALDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DEGMANLDVGLGDEDGAGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVKL
NQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFL
DAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCPNCKRPAQLPEHA
LLAEGFTQAQLDEGIQLHEAVGCDECTEGYKGRTGIYQVMPMTDEIATIVLAGGNALQIAEAAQAIGVNDLRQSALRKVA
AGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=734582 N8888_RS14750 WP_053517028.1 3215463..3217190(-) (pilB) [Stenotrophomonas maltophilia strain SG.Y2]
ATGAGCGTTGTTTCATCCCCCAACCTTGTCGGCATCACCGGGCTGGCGCGCCGCCTGGTCCAGGATGGTGCGCTGGACGA
GGCCACCGCCCGCGACGCCATGACCCGGGCCGCCGCCGCGCGGCAGCCGCTGCCGCAGTGGTTCGCGCAGAACAAGGTGG
TGGGGGCAGCACAGCTGGCCGCGGCCAATGCGGTTGAGTTCGGCATGCCGCTGTTCGACGTCTCGACCTTCGATGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAACTGCTGCGTAAGCACAACGTACTGCCGCTGTTCAAGCGTGGCGGCAAGCT
GTTCGTCGGCACCAGCAACCCGACCCACGCACTGGACGAGATCAAGTTCCACACCAACCTGGTGGTGGAGCCGATCCTGG
TCGACGAGGACCAGATCCGCCGCACTCTGGAACAGTGGCACGCCAGCCACGACACGCTGGGTGACGCGCTGGGCGGTGAC
GATGAGGGGATGGCCAACCTCGACGTGGGCCTGGGCGATGAGGATGGCGCGGGCGGCGACTCCGGCATCGATGCCAAGGG
CGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCGATCCGCAAGGGCGCGTCGGACATCCATTTCG
AGCCGTACGAAGACGATTACCGCGTGCGCCTGCGCATCGACGGGCTGTTGAAGATGGTCGCGCGCGCGCCGGTGAAGTTG
AACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCACAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGATGGCCGCAT
CAAGCTCAACCTGTCCAAGTCCAAGCAGATCGATTTCCGTGTGAGCACGTTGCCGACCCTGTTCGGCGAGAAAGTCGTGC
TGCGTATCCTCGACGGCAGTGCCGCCAAGCTGGGCATCGACAAGCTCGGCTATGAGCCCGACCAGCAGAAGCTGTTCCTG
GATGCGATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCTGTACACCGC
GCTGGGCATTCTCAACGACGAGACGCGCAACATCTCCACGGCGGAAGATCCGGTGGAAATCCGGCTGCCCGGCGTCAACC
AGGTGCAGCAGAACAACAAGCGCGGCATGACGTTCGCCGCTGCGTTGCGCTCGTTCCTGCGCCAGGATCCGGACATCATC
ATGGTCGGCGAAATCCGCGACCTGGAGACCGCCGAGATCGCCATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTCCAC
CCTGCATACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGTTCGG
TGACCCTGGTGATCGCCCAGCGTCTGGCGCGACGGCTGTGCCCGAACTGCAAGCGCCCGGCGCAGCTGCCGGAGCATGCA
TTGCTGGCCGAGGGCTTCACCCAGGCCCAGCTGGATGAGGGCATCCAGCTGCATGAGGCGGTGGGCTGTGATGAATGCAC
CGAGGGTTACAAGGGCCGCACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATTGCCACGATCGTGCTGGCCGGTG
GCAATGCGCTGCAGATTGCCGAAGCCGCGCAGGCGATCGGGGTCAATGACCTGCGGCAGTCGGCATTGAGGAAGGTCGCG
GCGGGCGTGACCAGCCTGGCCGAGATCAATCGCGTTACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.882

100

0.562
  pilB Acinetobacter baumannii D1279779

56.79

98.609

0.56
  pilB Legionella pneumophila strain ERS1305867

51.839

99.304

0.515
  pilB Vibrio cholerae strain A1552

49.645

98.087

0.487
  pilF Neisseria gonorrhoeae MS11

48.854

98.609

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

46.06

99.304

0.457
  pilB Vibrio campbellii strain DS40M4

45.694

98.957

0.452