Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   P4B08_RS28030 Genome accession   NZ_CP120704
Coordinates   6057161..6057784 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain SE5356     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6052161..6062784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P4B08_RS28010 (P4B08_28025) aroB 6052753..6053859 (-) 1107 WP_028681386.1 3-dehydroquinate synthase -
  P4B08_RS28015 (P4B08_28030) aroK 6053909..6054427 (-) 519 WP_003095833.1 shikimate kinase AroK -
  P4B08_RS28020 (P4B08_28035) pilQ 6054439..6056586 (-) 2148 WP_309267272.1 type 4a pilus secretin PilQ Machinery gene
  P4B08_RS28025 (P4B08_28040) comL/pilP 6056640..6057164 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  P4B08_RS28030 (P4B08_28045) pilO 6057161..6057784 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  P4B08_RS28035 (P4B08_28050) pilN 6057781..6058377 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  P4B08_RS28040 (P4B08_28055) pilM 6058377..6059441 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  P4B08_RS28045 (P4B08_28060) - 6059626..6062097 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=734029 P4B08_RS28030 WP_003103268.1 6057161..6057784(-) (pilO) [Pseudomonas aeruginosa strain SE5356]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=734029 P4B08_RS28030 WP_003103268.1 6057161..6057784(-) (pilO) [Pseudomonas aeruginosa strain SE5356]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401