Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   N5P16_RS14570 Genome accession   NZ_CP104734
Coordinates   3140581..3141732 (+) Length   383 a.a.
NCBI ID   WP_004922051.1    Uniprot ID   A0A7G2SB40
Organism   Acinetobacter baylyi strain JAT2044     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3135581..3146732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5P16_RS14550 (N5P16_14550) - 3136821..3137606 (+) 786 WP_004922040.1 transglutaminase-like domain-containing protein -
  N5P16_RS14555 (N5P16_14555) - 3137702..3138559 (-) 858 WP_011182166.1 undecaprenyl-diphosphate phosphatase -
  N5P16_RS14560 (N5P16_14560) - 3138696..3139385 (-) 690 WP_004922045.1 YggS family pyridoxal phosphate-dependent enzyme -
  N5P16_RS14565 (N5P16_14565) pilT 3139510..3140544 (+) 1035 WP_011182164.1 type IV pilus twitching motility protein PilT Machinery gene
  N5P16_RS14570 (N5P16_14570) pilU 3140581..3141732 (+) 1152 WP_004922051.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N5P16_RS14575 (N5P16_14575) fur 3141778..3142218 (-) 441 WP_004922054.1 ferric iron uptake transcriptional regulator -
  N5P16_RS14580 (N5P16_14580) - 3142330..3142728 (+) 399 WP_004922056.1 outer membrane protein assembly factor BamE -
  N5P16_RS14585 (N5P16_14585) - 3142784..3143095 (-) 312 WP_004922060.1 RnfH family protein -
  N5P16_RS14590 (N5P16_14590) - 3143092..3144177 (-) 1086 WP_004922063.1 hypothetical protein -
  N5P16_RS14595 (N5P16_14595) - 3144276..3145124 (-) 849 WP_026056952.1 class II glutamine amidotransferase -
  N5P16_RS14600 (N5P16_14600) - 3145232..3146011 (-) 780 WP_004922069.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 43616.87 Da        Isoelectric Point: 6.6084

>NTDB_id=731709 N5P16_RS14570 WP_004922051.1 3140581..3141732(+) (pilU) [Acinetobacter baylyi strain JAT2044]
MDFNDLLNLMAKHKASDLFITAGVEPSLKINGEITPVSKTKLDGDMIAQLIDSITTQKQRQEFIETHECNFAILNREKNE
RFRVSAFQQRDMPGMVIRRIETKIPSPDDLHLPPILKELAMSKRGIIIFSGATGTGKSTSLASMIQHRNQHSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYALAFAETGHLVFATLHANNANQTLER
IIHFFDADRHQQIFMDLSLNLKAVISQQLIPTHDGQSRRAAVEVLINTPLLSDYIRQGEIYKIKELMARSGELGMQTFDQ
ALFDLYDHQHISYADALKHADSPNNLRLQIKLSEEANKPLDNRERMSEMERKMTFDGQRRNLK

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=731709 N5P16_RS14570 WP_004922051.1 3140581..3141732(+) (pilU) [Acinetobacter baylyi strain JAT2044]
ATGGACTTTAATGATTTACTCAATTTGATGGCAAAACACAAAGCATCTGATTTGTTTATTACAGCAGGTGTAGAACCATC
TTTAAAAATTAACGGTGAAATTACCCCCGTCTCTAAAACCAAACTCGATGGAGATATGATCGCTCAGTTGATTGATTCGA
TTACAACGCAAAAGCAACGACAAGAATTCATTGAAACGCATGAATGTAATTTTGCCATCTTAAATCGTGAAAAAAACGAA
CGTTTCCGGGTGAGCGCATTTCAACAGCGTGACATGCCTGGTATGGTGATTCGTCGTATTGAGACCAAGATCCCCTCTCC
TGACGATTTACATCTACCTCCAATTTTAAAAGAACTGGCCATGAGCAAACGTGGCATTATTATTTTTTCTGGGGCAACTG
GTACAGGAAAATCCACGTCTTTGGCATCCATGATTCAACATCGTAATCAGCACTCAAAAGGTCATATTATTACTATTGAG
GATCCGATTGAATTTATACATGAGCATGCTGGATGTATTATCACTCAGCGTGAAGTCGGCATTGATACAGATTCTTTTGA
AATCGCGCTTAAAAATACGTTGCGCCAGGCGCCTGATGTGATTCTGATTGGTGAAATTCGCTCACGTGAAGTGATGGACT
ATGCTTTAGCTTTTGCAGAAACTGGCCATTTAGTATTTGCAACTTTACATGCCAATAATGCCAATCAAACCTTAGAACGG
ATTATTCACTTCTTTGATGCAGATCGACATCAGCAAATTTTTATGGATTTATCGCTCAATCTTAAAGCGGTGATTTCACA
ACAGCTTATTCCAACCCATGATGGTCAAAGTCGACGTGCTGCCGTCGAAGTGCTGATTAATACGCCTTTACTGTCAGATT
ACATTCGTCAAGGTGAAATCTATAAAATTAAGGAATTAATGGCTCGTTCTGGAGAACTTGGCATGCAAACCTTTGATCAG
GCACTTTTTGATTTATATGATCATCAGCACATCAGTTATGCAGATGCGCTCAAGCATGCAGACTCGCCTAATAATTTACG
TCTACAGATCAAGCTTTCGGAAGAAGCCAACAAACCCCTAGATAACAGAGAAAGAATGTCCGAAATGGAACGGAAAATGA
CTTTTGATGGTCAACGCCGTAATCTCAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G2SB40

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

100

100

1
  pilU Pseudomonas stutzeri DSM 10701

65.266

93.211

0.608
  pilU Vibrio cholerae strain A1552

54.701

91.645

0.501
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.331

94.517

0.381