Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N4261_RS22750 Genome accession   NZ_CP104562
Coordinates   5483235..5485010 (+) Length   591 a.a.
NCBI ID   WP_261757525.1    Uniprot ID   -
Organism   Roseateles amylovorans strain BIM B-1768     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5478235..5490010
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4261_RS22725 (N4261_22750) cgtA 5479187..5480248 (-) 1062 Protein_4464 Obg family GTPase CgtA -
  N4261_RS22730 (N4261_22755) rpmA 5480468..5480725 (-) 258 WP_261757523.1 50S ribosomal protein L27 -
  N4261_RS22735 (N4261_22760) rplU 5480762..5481073 (-) 312 WP_261757524.1 50S ribosomal protein L21 -
  N4261_RS22740 (N4261_22765) - 5481648..5482577 (+) 930 WP_261760823.1 polyprenyl synthetase family protein -
  N4261_RS22750 (N4261_22775) pilB 5483235..5485010 (+) 1776 WP_261757525.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N4261_RS22755 (N4261_22780) pilC 5485162..5486397 (+) 1236 WP_261757526.1 type II secretion system F family protein Machinery gene
  N4261_RS22760 (N4261_22785) - 5486398..5487342 (+) 945 WP_261757527.1 A24 family peptidase -
  N4261_RS22765 (N4261_22790) coaE 5487517..5488116 (+) 600 WP_261757528.1 dephospho-CoA kinase -
  N4261_RS22770 (N4261_22795) zapD 5488216..5488971 (+) 756 WP_261760824.1 cell division protein ZapD -
  N4261_RS22775 (N4261_22800) - 5489025..5489249 (+) 225 WP_261757529.1 DNA gyrase inhibitor YacG -
  N4261_RS22780 (N4261_22805) - 5489348..5489968 (-) 621 WP_261757530.1 HAD family phosphatase -

Sequence

Protein


Download         Length: 591 a.a.        Molecular weight: 64649.33 Da        Isoelectric Point: 6.8755

>NTDB_id=730598 N4261_RS22750 WP_261757525.1 5483235..5485010(+) (pilB) [Roseateles amylovorans strain BIM B-1768]
MCRPAARRRHVLTKHSSSVDTTLAEPPQSALSGVARVLVHAGKLQPKAAEDLVRQAREKKTSFVNALIAAGTVSSADLAH
TLSKALAMPLLDLNAVDAHKLPQNIIDNKLAAQYHVVVLARRGNRLFVGGADPTEQEVVERIKFATQLTPEWVIVEHDKL
GKLLSATGASAAEALEAIAGEDFDFDIAEEESAPQEESQEIGDVEDAPVVRFLQKMLVDAINMRASDLHFEPYEYHYRVR
FRVDGELREITQPPIAIKDKLSSRIKVISRMDIAERRVPQDGRMKLKFGSKAIDFRVSTLPTLFGEKIVIRILDPSSAKL
GIEALGYEKMEKERLLACIVRPYGMVLVTGPTGSGKTVSLYTCLNILNQPGTNISTVEDPAEINLPGINQVNVNDKAGLT
FSAALKAFLRQDPDIIMVGEIRDLETADIAIKAAQTGHMVMSTLHTNDAPTTLTRLLNMGVAPFNIASSVLLITAQRLAR
RLCENCKAPAEYPREALLRAGYLETDLDGSWKPYRAVGCANCNNGYRGRVGLYQVMPISEAIQRIILSEGTALDIADQAR
KDGVRDLRQSGLVKVRAGVTTLEEVLAATNE

Nucleotide


Download         Length: 1776 bp        

>NTDB_id=730598 N4261_RS22750 WP_261757525.1 5483235..5485010(+) (pilB) [Roseateles amylovorans strain BIM B-1768]
ATGTGCCGCCCGGCAGCGCGCCGCCGCCACGTACTAACAAAGCACTCCTCATCCGTGGACACCACACTGGCCGAACCACC
TCAGTCCGCCCTTTCGGGCGTCGCCCGCGTGCTCGTGCACGCCGGCAAGCTTCAGCCCAAGGCGGCCGAGGACCTGGTCC
GTCAGGCGCGGGAGAAAAAGACCAGCTTCGTCAATGCGCTGATCGCCGCCGGCACCGTGTCCTCCGCCGATCTGGCGCAC
ACGCTGTCCAAGGCACTGGCCATGCCGCTGCTCGACCTGAATGCGGTCGACGCGCACAAGCTGCCCCAGAACATCATCGA
CAACAAGCTGGCCGCCCAGTACCACGTGGTGGTGCTGGCGCGTCGCGGCAACCGGTTGTTCGTCGGCGGCGCCGATCCGA
CCGAGCAGGAAGTCGTCGAGCGCATCAAGTTCGCCACCCAGCTCACGCCGGAATGGGTGATCGTCGAGCATGACAAGCTC
GGCAAGCTGCTCAGCGCCACCGGCGCTTCGGCGGCTGAAGCGCTGGAAGCGATCGCCGGCGAGGACTTCGACTTCGACAT
CGCCGAGGAAGAATCGGCCCCCCAGGAAGAATCGCAGGAAATCGGCGACGTCGAAGACGCCCCGGTGGTGCGCTTCCTGC
AGAAGATGCTGGTGGACGCCATCAACATGCGGGCGTCCGACCTCCACTTCGAGCCCTACGAATACCACTACCGCGTCCGC
TTCCGGGTGGACGGCGAACTGCGGGAAATCACCCAGCCGCCGATCGCGATCAAGGACAAGCTGTCCTCGCGGATCAAGGT
CATCTCCCGGATGGACATCGCCGAGCGCCGCGTCCCGCAAGACGGCCGGATGAAGCTCAAGTTCGGCAGCAAGGCGATCG
ACTTCCGGGTCTCGACCCTGCCGACCCTGTTCGGCGAGAAGATCGTGATCCGGATTCTCGACCCGTCCTCCGCCAAGCTC
GGCATCGAGGCGCTGGGCTACGAGAAGATGGAAAAGGAACGCCTGCTGGCCTGCATCGTGCGGCCGTACGGCATGGTGCT
GGTCACCGGCCCGACCGGTTCCGGCAAGACGGTGTCGCTCTACACCTGCCTGAACATCCTGAACCAGCCCGGCACCAACA
TCTCGACGGTGGAAGACCCGGCGGAAATCAACCTGCCCGGCATCAACCAGGTCAACGTCAACGACAAGGCCGGCCTGACC
TTCTCGGCCGCGCTCAAGGCCTTCCTGCGTCAGGATCCCGACATCATCATGGTCGGTGAGATCCGGGACCTGGAAACCGC
CGACATCGCCATCAAGGCCGCGCAGACCGGCCACATGGTGATGTCCACGCTGCACACCAATGACGCGCCAACCACCCTGA
CCCGTCTGCTCAACATGGGCGTGGCGCCGTTCAACATCGCTTCCAGCGTGCTGCTGATCACCGCTCAGCGGCTGGCGCGA
CGCCTGTGTGAGAACTGCAAGGCCCCCGCGGAGTACCCACGCGAGGCCCTGCTGCGCGCCGGCTATCTCGAAACCGACCT
CGACGGTTCGTGGAAGCCCTACCGCGCCGTGGGTTGCGCGAATTGCAACAATGGTTATCGGGGCCGGGTCGGCCTTTACC
AAGTGATGCCGATTAGTGAAGCAATTCAGCGCATCATCCTGTCCGAGGGAACCGCCCTGGACATCGCCGACCAGGCACGC
AAAGACGGCGTGCGGGATCTGCGACAGTCCGGACTGGTCAAGGTTCGAGCAGGTGTGACCACCCTGGAAGAAGTGCTGGC
AGCGACCAATGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(215-481)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(67-170)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.996

95.262

0.514
  pilF Neisseria gonorrhoeae MS11

53.345

96.108

0.513
  pilB Acinetobacter baylyi ADP1

52.182

96.954

0.506
  pilB Legionella pneumophila strain ERS1305867

49.288

95.093

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

44.737

96.447

0.431
  pilB Vibrio campbellii strain DS40M4

45.018

95.093

0.428
  pilB Vibrio cholerae strain A1552

44.681

95.431

0.426
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.84

96.277

0.374