Detailed information NTG0728728

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: N4T40_RS04845
• Genome accession: NZ_CP104335
• Coordinates: 919831..920889 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain 2021CK-01408
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 914831..925889

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N4T40_RS04820 (N4T40_04820)	aroK	915106..915675 (-)	570	WP_000643712.1	shikimate kinase AroK	-
N4T40_RS04825 (N4T40_04825)	pilQ	915687..917852 (-)	2166	WP_001017038.1	type IV pilus secretin PilQ family protein	Machinery gene
N4T40_RS04830 (N4T40_04830)	pilP	917915..918442 (-)	528	WP_000695058.1	pilus assembly protein PilP	Machinery gene
N4T40_RS04835 (N4T40_04835)	pilO	918453..919193 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
N4T40_RS04840 (N4T40_04840)	pilN	919190..919831 (-)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
N4T40_RS04845 (N4T40_04845)	pilM	919831..920889 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
N4T40_RS04850 (N4T40_04850)	ponA	921051..923606 (+)	2556	WP_000736683.1	penicillin-binding protein PBP1a	-
N4T40_RS04855 (N4T40_04855)	-	923624..924448 (-)	825	WP_086242007.1	putative RNA methyltransferase	-
N4T40_RS04860 (N4T40_04860)	-	924451..924783 (-)	333	WP_000169601.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=728728 N4T40_RS04845 WP_002014066.1 919831..920889(-) (pilM) [Acinetobacter baumannii strain 2021CK-01408]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=728728 N4T40_RS04845 WP_002014066.1 919831..920889(-) (pilM) [Acinetobacter baumannii strain 2021CK-01408]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAATGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCGGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCCTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACTATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCAGAGCGAAAAAAGATCGTTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGCTCACTACAATTTTTCTTTTCCTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCATTCTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423