Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K7573_RS16055 Genome accession   NZ_CP104292
Coordinates   3483960..3485693 (-) Length   577 a.a.
NCBI ID   WP_227843515.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain ACYCe.8N     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3478960..3490693
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K7573_RS16040 (K7573_16040) sucC 3479259..3480428 (-) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -
  K7573_RS16045 (K7573_16045) - 3480786..3482399 (+) 1614 WP_005414304.1 ATP-binding protein -
  K7573_RS16050 (K7573_16050) pilR 3482460..3483833 (+) 1374 WP_005410795.1 sigma-54 dependent transcriptional regulator Regulator
  K7573_RS16055 (K7573_16055) pilB 3483960..3485693 (-) 1734 WP_227843515.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K7573_RS16060 (K7573_16060) - 3485916..3486323 (-) 408 WP_227843516.1 pilin -
  K7573_RS16065 (K7573_16065) - 3486476..3486913 (-) 438 WP_227843517.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K7573_RS16070 (K7573_16070) pilC 3487266..3488525 (+) 1260 WP_049460357.1 type II secretion system F family protein Machinery gene
  K7573_RS16075 (K7573_16075) - 3488533..3489396 (+) 864 WP_227843518.1 A24 family peptidase -
  K7573_RS16080 (K7573_16080) coaE 3489408..3490019 (+) 612 WP_227843519.1 dephospho-CoA kinase -
  K7573_RS16085 (K7573_16085) - 3490279..3490665 (-) 387 WP_024956768.1 hypothetical protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62303.23 Da        Isoelectric Point: 5.8722

>NTDB_id=728354 K7573_RS16055 WP_227843515.1 3483960..3485693(-) (pilB) [Stenotrophomonas maltophilia strain ACYCe.8N]
MNAVTTANLVGITGLARRLVQDGALDEATARDAMAKAAAARQPLPTWFAQNKLVNASQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DDGMGNLDVAAGDEDLGATGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRPSSLPE
HALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGVYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKK
AAAGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=728354 K7573_RS16055 WP_227843515.1 3483960..3485693(-) (pilB) [Stenotrophomonas maltophilia strain ACYCe.8N]
ATGAACGCTGTCACCACCGCCAATCTTGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTGCAGGACGGGGCCCTGGACGA
GGCCACCGCCCGCGACGCCATGGCCAAGGCCGCCGCCGCGCGCCAGCCACTGCCGACGTGGTTCGCGCAGAACAAGCTGG
TCAATGCCTCGCAGCTGGCCGCCGCCAACGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACTTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGCGGTGGCAAGCT
GTTCGTCGGCACCAGCAACCCGACCCACTCGCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTCGAGCCGATCCTGG
TCGACGAGGACCAGATCCGGCGCACGCTGGAACAGTGGCACGCCAGCCATGACACCCTCGGCGACGCCCTGGGTGGCGAT
GACGATGGCATGGGCAACCTGGACGTGGCGGCCGGCGACGAGGATCTGGGCGCCACCGGCGGCGATTCCGGCATCGATGC
CAAGGGTGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCGATCCGCAAGGGCGCCTCGGACATCC
ACTTCGAGCCCTACGAAGACGATTACCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTGGCGCGCGCACCGGTG
AAGCTGAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCACAGCTGGACATCGCCGAGAAGCGCGTCCCGCAGGACGG
CCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGG
TGGTGCTGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTG
TTCCTGGATGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACCGTGTCGCTGTA
TACCGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCTACTGCCGAAGATCCGGTGGAAATCCGCCTGCCCGGCG
TCAACCAGGTACAGCAGAACAACAAGCGCGGCATGACGTTCGCCGCGGCGCTGCGCTCGTTCCTGCGACAGGACCCGGAC
ATCATCATGGTCGGCGAAATCCGTGACCTGGAAACGGCGGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCT
GTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCA
GCTCGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGCCGCCTGTGCGGTAACTGCAAGCGTCCGTCCAGCCTGCCCGAA
CATGCGCTGCTGGCCGAAGGCTTCACCCAGGCGCAGCTTGATGCGGGCATCGAGCTGTACGAGGCCGTGGGCTGCGACGA
ATGCACCGAGGGCTACAAGGGCCGGACCGGCGTCTACCAGGTCATGCCGATGACCGATGAGATCGCCACCATCGTGCTGG
CCGGTGGCAATGCGCTGCAGATTGCCGAGGCGGCGCAGCAGATCGGGGTGAATGACCTGCGCCAGTCGGCATTGAAGAAG
GCGGCGGCGGGTGTTACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-150)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.043

100

0.558
  pilB Acinetobacter baumannii D1279779

55.172

100

0.555
  pilB Legionella pneumophila strain ERS1305867

51.754

98.787

0.511
  pilF Neisseria gonorrhoeae MS11

48.596

98.787

0.48
  pilB Vibrio cholerae strain A1552

48.501

98.267

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

45.455

99.133

0.451
  pilB Vibrio campbellii strain DS40M4

45.167

98.614

0.445