Detailed information NTG0726959

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: N0B71_RS05720
• Genome accession: NZ_CP104011
• Coordinates: 1256224..1257288 (-)
• Length: 354 a.a.
• NCBI ID: WP_017520515.1
• Uniprot ID: A0A5R9R179
• Organism: Pseudomonas sp. GCEP-101 isolate Gut
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1251224..1262288

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N0B71_RS05695	aroK	1251795..1252313 (-)	519	WP_259757759.1	shikimate kinase AroK	-
N0B71_RS05700	pilQ	1252322..1254400 (-)	2079	WP_259757760.1	type IV pilus secretin PilQ	Machinery gene
N0B71_RS05705	pilP	1254454..1254978 (-)	525	WP_259757761.1	type 4a pilus biogenesis lipoprotein PilP	-
N0B71_RS05710	pilO	1254975..1255637 (-)	663	WP_259757763.1	type 4a pilus biogenesis protein PilO	-
N0B71_RS05715	pilN	1255634..1256224 (-)	591	WP_259757764.1	type 4a pilus biogenesis protein PilN	-
N0B71_RS05720	pilM	1256224..1257288 (-)	1065	WP_017520515.1	pilus assembly protein PilM	Machinery gene
N0B71_RS05725	-	1257475..1259904 (+)	2430	WP_259757765.1	penicillin-binding protein 1A	-
N0B71_RS05730	-	1260081..1260548 (+)	468	WP_259757766.1	hypothetical protein	-
N0B71_RS05735	-	1260542..1261036 (-)	495	WP_259757767.1	M48 family metallopeptidase	-
N0B71_RS05740	-	1261086..1261856 (-)	771	WP_259757768.1	class I SAM-dependent methyltransferase	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37747.01 Da
• Isoelectric Point: 4.4445

>NTDB_id=726959 N0B71_RS05720 WP_017520515.1 1256224..1257288(-) (pilM) [Pseudomonas sp. GCEP-101 isolate Gut]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVVAKARTAVKTSAV
AVAGSAVITKTIEMDGGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGTSARNADRVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAFSLLGDQLGGNADELTVAVVDIGATMTTLSVLHHGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMVLSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=726959 N0B71_RS05720 WP_017520515.1 1256224..1257288(-) (pilM) [Pseudomonas sp. GCEP-101 isolate Gut]
GTGCTAGGGCTCTTCAATAAGAAAGCGAACACGCTGCTGGGGATAGACATCAGTTCGACTTCGGTCAAACTTCTCGAATT
GAGCCGCTCTGGAGGCCGCTACAAGGTAGAGGCCTATGCTGTGGAGCCGCTCCCGCCCAATGCAGTGGTAGAAAAGAACA
TCGCTGAGCTGGAGGGTGTCGGCCAGGCGTTGTCACGCGTGGTCGCCAAGGCGCGGACCGCGGTGAAGACGTCTGCGGTC
GCCGTTGCCGGCTCGGCGGTCATCACTAAAACCATCGAGATGGACGGCGGACTCTCGGACGATGAGTTGGAAAACCAGCT
CAAGATCGAGGCCGATCAGTACATCCCCTATCCCCTCGAAGAGGTTGCGATCGACTTCGAGGTGCAGGGCACGTCCGCGC
GCAACGCGGATCGCGTCGATGTTCTGCTTGCCGCTTGCCGGAAGGAGAACGTCGAGGTGCGCGAAGCTGCGCTGGCGCTC
GCGGGCCTGACGGCCAAGGTCGTCGATGTTGAAGCCTATGCGCTGGAGCGAGCGTTCAGTCTGCTGGGAGACCAGTTGGG
CGGCAATGCCGACGAGCTCACAGTTGCGGTGGTGGACATCGGCGCCACCATGACCACGCTCAGCGTCCTGCACCATGGTC
GCACCATCTATACCCGCGAACAGCTCTTTGGTGGGCGCCAGCTGACCGAGGAAATCCAGCGTCGCTACGGCCTGTCGGTC
GAGGAAGCCGGTCTTGCGAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTGCTGCAACCGTTCAAGGACGC
CGTGGTACAGCAGGTGTCCCGTTCGCTGCAGTTCTTCTTCGCGGCCGGCCAGTTCAACGACGTGGATTACATCCTGCTCG
CCGGTGGCACGGCGTCCATCCCCGACCTGGATCGCCTGATCCAGCAGAAGATCGGCACTCCGACCCTGGTAGCCAACCCG
TTCGCCGACATGGTGCTGAGCGGCAAGGTCAACGCCGGTGCGCTGGCCAGTGATGCGCCGGCGCTGATGATTGCCTGCGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A5R9R179

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	57.345	100	0.573
comM	Acinetobacter nosocomialis M2	57.345	100	0.573
comM	Acinetobacter baylyi ADP1	53.39	100	0.534
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466