Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   N0B71_RS05700 Genome accession   NZ_CP104011
Coordinates   1252322..1254400 (-) Length   692 a.a.
NCBI ID   WP_259757760.1    Uniprot ID   -
Organism   Pseudomonas sp. GCEP-101 isolate Gut     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1247322..1259400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N0B71_RS05685 - 1248932..1250632 (-) 1701 WP_259757757.1 AAA family ATPase -
  N0B71_RS05690 aroB 1250639..1251745 (-) 1107 WP_259757758.1 3-dehydroquinate synthase -
  N0B71_RS05695 aroK 1251795..1252313 (-) 519 WP_259757759.1 shikimate kinase AroK -
  N0B71_RS05700 pilQ 1252322..1254400 (-) 2079 WP_259757760.1 type IV pilus secretin PilQ Machinery gene
  N0B71_RS05705 pilP 1254454..1254978 (-) 525 WP_259757761.1 type 4a pilus biogenesis lipoprotein PilP -
  N0B71_RS05710 pilO 1254975..1255637 (-) 663 WP_259757763.1 type 4a pilus biogenesis protein PilO -
  N0B71_RS05715 pilN 1255634..1256224 (-) 591 WP_259757764.1 type 4a pilus biogenesis protein PilN -
  N0B71_RS05720 pilM 1256224..1257288 (-) 1065 WP_017520515.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74137.05 Da        Isoelectric Point: 5.0897

>NTDB_id=726958 N0B71_RS05700 WP_259757760.1 1252322..1254400(-) (pilQ) [Pseudomonas sp. GCEP-101 isolate Gut]
MNKTLSRLGISVLAAVFAPTLLAANLQSVDVAALPGDRVELKLGFDEPVAAPRGYTIEQPARIALDLPGVQNKLGSKNRE
LGVGNARSLTVVEAKDRTRLIVNLATLVPYTTRAEGSNLYVVVGAGGAPAASYASAAPSPAPAAKPAQPKPYIPAGKAIR
NIDFQRGEQGEGNVVIDLSDPTVSPDIQEQGGKIRLDFPRTQLPDNLRVRLDVKDFATPVQLVNASAVGDKASITIEPTG
LYDYLVYQTDNRMTVSIKPLTQDDAEKRKKDSFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQN
VPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELEAQKQISELAPLRRELIQVNYAKAADIAKLFSSVTGTGG
GAPGVAAAAQGDNRGSITVDDRTNSIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYTKELGVNWSGSLSGH
GNFDVSGGTNSTGNTFVDLGASNTTPTSGISIGFTTSNTILDLQLNAMEKTGNGEVVSQPKVVTSDKETAKILKGSEIPY
QEASSSGATSVSFKEAALSLEVTPQITPDNRIIMEVKVNKDEPDYANALNGVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQTKSTAKVPFLGDVPMIGRLFKNDYVTDSKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=726958 N0B71_RS05700 WP_259757760.1 1252322..1254400(-) (pilQ) [Pseudomonas sp. GCEP-101 isolate Gut]
ATGAATAAAACGCTTTCCCGCCTGGGCATTTCCGTGCTCGCGGCCGTATTCGCACCGACCCTGCTGGCTGCAAACCTGCA
GAGCGTCGACGTGGCGGCCCTGCCGGGCGACCGCGTGGAACTCAAGCTCGGCTTCGATGAGCCAGTGGCGGCGCCACGTG
GCTACACCATCGAGCAGCCCGCGCGCATTGCGCTGGACCTGCCGGGTGTGCAGAACAAGCTGGGCAGCAAGAACCGCGAA
CTGGGGGTGGGCAACGCCCGCAGCCTGACCGTGGTCGAGGCGAAGGACCGTACCCGCCTCATCGTCAACCTGGCGACGCT
GGTGCCGTACACCACCCGTGCCGAGGGTTCCAACCTGTACGTCGTGGTCGGCGCCGGCGGCGCGCCAGCGGCCTCCTACG
CTTCCGCGGCGCCGTCTCCGGCTCCGGCGGCCAAGCCGGCGCAGCCCAAGCCGTATATCCCGGCCGGCAAGGCGATCCGC
AATATCGATTTCCAGCGTGGCGAGCAGGGTGAAGGCAACGTCGTCATCGACCTGTCCGATCCGACCGTCAGTCCCGACAT
CCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCCCGCGCACCCAGCTGCCGGACAACCTGCGCGTGCGCCTGGACGTGA
AGGACTTCGCGACCCCGGTGCAACTGGTCAACGCCTCCGCCGTTGGCGACAAGGCCAGCATCACCATCGAGCCGACCGGC
CTGTACGACTACCTCGTCTATCAGACCGACAATCGCATGACCGTCAGCATCAAGCCGCTGACCCAGGACGATGCCGAGAA
GCGCAAGAAGGACAGCTTCGCCTATACCGGCGAAAAACTGTCCCTGAACTTCCAGGACATCGACGTGCGTTCGGTTCTGC
AGCTGATCGCCGACTTCACCGACCTGAACCTCGTCGCCTCGGACACCGTGCAGGGCAACATCACTCTGCGCCTGCAGAAC
GTGCCGTGGGACCAGGCGCTGGATCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGGTCGGCAACGTCCTGCTGGT
CGCGCCGGCTGACGAGATCGCCGCCCGCGAACGCCAGGAGCTGGAAGCGCAGAAGCAGATTTCCGAACTGGCGCCCCTGC
GCCGTGAACTGATCCAGGTGAACTACGCCAAGGCGGCGGACATCGCCAAGCTGTTCAGCTCCGTCACCGGCACCGGTGGT
GGCGCGCCCGGCGTTGCTGCGGCAGCCCAGGGCGACAACCGCGGTTCGATCACCGTGGACGATCGCACCAACAGCATCAT
CGCCTACCAGACCCAGGATCGCCTGGACGAGCTGCGCCGCATCGTCGCCCAGCTGGACATCCCGGTGCGCCAGGTGATGA
TCGAGGCGCGCATCGTCGAAGCCAACGTCGACTACACCAAGGAGCTGGGCGTGAACTGGAGCGGTTCGCTGTCCGGTCAC
GGCAACTTCGATGTGTCCGGCGGCACCAACAGCACGGGCAATACCTTCGTCGACCTCGGCGCGTCGAACACCACGCCGAC
CTCGGGCATCAGCATCGGCTTCACCACCAGCAACACCATCCTGGACCTGCAGCTCAATGCCATGGAGAAGACCGGTAACG
GTGAAGTGGTTTCCCAGCCGAAGGTCGTCACCTCCGACAAGGAAACCGCGAAGATCCTCAAGGGCTCGGAGATTCCGTAC
CAGGAAGCCAGCTCCAGCGGTGCGACCTCGGTGTCCTTCAAGGAAGCGGCGCTGTCCCTGGAAGTGACCCCGCAGATCAC
GCCGGACAACCGCATCATCATGGAAGTGAAGGTCAACAAGGACGAGCCGGACTACGCCAACGCCCTGAACGGCGTACCGC
CGATCAAGAAGAACGAGGTGAACGCGAAGGTGCTGGTCAACGATGGCGAAACCATCGTGATCGGTGGCGTGTTCTCCAAC
ACCCAGACCAAATCGACGGCGAAGGTGCCGTTCCTGGGCGACGTGCCGATGATCGGCCGCCTGTTCAAGAACGACTACGT
GACCGACAGCAAGAACGAGCTGCTGGTTTTCCTTACCCCGCGGATCATGAATAATCAGGCCATTGCCATCGGCCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(527-683)

AMIN domain (AMIN)

(40-121)

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-451)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

78.532

100

0.819
  comQ Acinetobacter baylyi ADP1

42.402

99.855

0.423
  pilQ Acinetobacter baumannii strain A118

39.728

100

0.422
  pilQ Acinetobacter baumannii D1279779

39.728

100

0.422
  pilQ Legionella pneumophila strain Lp02

39.118

98.266

0.384
  pilQ Legionella pneumophila strain ERS1305867

39.118

98.266

0.384