Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   NYP20_RS02070 Genome accession   NZ_CP103965
Coordinates   457236..458300 (+) Length   354 a.a.
NCBI ID   WP_259498546.1    Uniprot ID   -
Organism   Pseudomonas sp. N3-W     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 452236..463300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYP20_RS02060 (NYP20_02060) - 452971..454239 (+) 1269 WP_259498541.1 malic enzyme-like NAD(P)-binding protein -
  NYP20_RS02065 (NYP20_02065) - 454595..457018 (-) 2424 WP_409077940.1 penicillin-binding protein 1A -
  NYP20_RS02070 (NYP20_02070) comM 457236..458300 (+) 1065 WP_259498546.1 pilus assembly protein PilM Machinery gene
  NYP20_RS02075 (NYP20_02075) - 458300..458866 (+) 567 WP_259498548.1 PilN domain-containing protein -
  NYP20_RS02080 (NYP20_02080) pilO 458863..459486 (+) 624 WP_259498550.1 type 4a pilus biogenesis protein PilO -
  NYP20_RS02085 (NYP20_02085) - 459483..460010 (+) 528 WP_259498552.1 pilus assembly protein PilP -
  NYP20_RS02090 (NYP20_02090) pilQ 460024..462108 (+) 2085 WP_259498553.1 type IV pilus secretin PilQ Machinery gene
  NYP20_RS02095 (NYP20_02095) aroK 462113..462631 (+) 519 WP_259498554.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38069.55 Da        Isoelectric Point: 4.6182

>NTDB_id=726664 NYP20_RS02070 WP_259498546.1 457236..458300(+) (comM) [Pseudomonas sp. N3-W]
MLGLFNKKTGTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRVLIKARTGIRNVAV
AVAGSAVITKIIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVVGVSERNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFRDALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDQLIEQRLGTPTQVANP
FADMTLSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=726664 NYP20_RS02070 WP_259498546.1 457236..458300(+) (comM) [Pseudomonas sp. N3-W]
GTGCTAGGACTCTTCAATAAAAAGACCGGAACGCTTCTGGGGATCGATATCAGCTCCACGTCGGTGAAGCTTTTGGAGCT
GAGCCGCCAGGGTGATCGCTATCGGGTCGAGGCTTATGCCGTCGAGCCGTTACCGGCCAATGCCGTTGTCGAAAAGAACA
TTGCCGAGCTTGAGGGCGTGGGTCAGGCGCTCTCGCGGGTGCTGATCAAGGCCCGAACCGGGATCAGGAACGTCGCGGTG
GCCGTGGCCGGTTCAGCGGTGATTACCAAGATCATTGAAATGGACGCCGGTCTTTCCGACGACGAGCTGGAGAATCAGCT
CAAGATCGAAGCCGATCAATACATTCCCTACCCTCTGGACGAAGTCGCCATCGACTTTGAAGTCGTGGGCGTGTCCGAGC
GCAATCCGGAGCGGGTCAATGTGCTACTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTTCGCGAAGCCGCCCTGGCATTG
GCCGGGCTGACCGCCCGGGTGGTGGACGTCGAGGCCTATGCGCTGGAGCGCTCGTTTGGCCTGCTGGCGACGCAGCTGGC
GGCATCCCAGGAGCGTCTGACTGTCGCGGTGGTGGACATCGGCGCGACCATGACCACCCTGAGCGTGCTGCACAACGGTC
GCATCATCTATACCCGCGAGCAACTGTTCGGTGGTCGACAACTGACCGAAGAGATCCAGCGTCGTTACGGCCTGACGGTG
GAGCAGGCGGGGCTTGCAAAAAAACAGGGTGGCTTGCCGGACGATTATGTCAGCGAAGTGTTGCAGCCGTTTCGCGACGC
CCTGGTGCAGCAGGTGTCCCGCTCGTTGCAGTTTTTCTTCGCCTCGGGCCAATACAACGCCGTCGACCACATTCTGCTGG
CCGGTGGCACCGCCTCGGTGCCGGGGCTCGACCAGTTGATCGAACAGCGGCTGGGCACGCCGACCCAGGTGGCCAATCCC
TTTGCCGACATGACGCTCAGCAGCAAGGTCAATGCCGGTGCGCTGGCCAGTGATGCGCCTGCGTTGATGATTGCCTGCGG
GCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

54.802

100

0.548
  pilM Legionella pneumophila strain ERS1305867

48.023

100

0.48