Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NYP20_RS02090 Genome accession   NZ_CP103965
Coordinates   460024..462108 (+) Length   694 a.a.
NCBI ID   WP_259498553.1    Uniprot ID   -
Organism   Pseudomonas sp. N3-W     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 455024..467108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYP20_RS02070 (NYP20_02070) comM 457236..458300 (+) 1065 WP_259498546.1 pilus assembly protein PilM Machinery gene
  NYP20_RS02075 (NYP20_02075) - 458300..458866 (+) 567 WP_259498548.1 PilN domain-containing protein -
  NYP20_RS02080 (NYP20_02080) pilO 458863..459486 (+) 624 WP_259498550.1 type 4a pilus biogenesis protein PilO -
  NYP20_RS02085 (NYP20_02085) - 459483..460010 (+) 528 WP_259498552.1 pilus assembly protein PilP -
  NYP20_RS02090 (NYP20_02090) pilQ 460024..462108 (+) 2085 WP_259498553.1 type IV pilus secretin PilQ Machinery gene
  NYP20_RS02095 (NYP20_02095) aroK 462113..462631 (+) 519 WP_259498554.1 shikimate kinase AroK -
  NYP20_RS02100 (NYP20_02100) aroB 462783..463883 (+) 1101 WP_259498556.1 3-dehydroquinate synthase -
  NYP20_RS02105 (NYP20_02105) - 463894..465474 (+) 1581 WP_259498558.1 AAA family ATPase -

Sequence

Protein


Download         Length: 694 a.a.        Molecular weight: 75483.87 Da        Isoelectric Point: 5.8400

>NTDB_id=726665 NYP20_RS02090 WP_259498553.1 460024..462108(+) (pilQ) [Pseudomonas sp. N3-W]
MNRIFSTLGLSLWIALLSPMVQAASLNSLDVAALPGDRIELKLSFDAPPPPPKGYTTESPARIALDLPGVANKLTNKNRD
LGRGNARSATVVEANDRTRLIISLTQLAPYSTRVEGNNLFVVVGQGAKVNVPKPVASAPRAATVKPAAGKTWAPVTKAIR
GVDFQRGTQGEGNVVIDLSDPSIAPDIQERDGKIILGFARTQLPEPLRVRLDVKDFATPVQFVNAAATGDRATISIEPVG
AYEYSTYQTDNKLTVSIRAVTADDLQKRNAERFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAKDIAELFRSVTNKKQ
SETDERGSITVDERTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGV
TNGSTTTIGTPGSTSTNSPFVDMGTAGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEI
PYQEASSSGATSVAFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVF
SNTQSKIVDKVPFLGDVPYLGRLFRRDVVSEQKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2085 bp        

>NTDB_id=726665 NYP20_RS02090 WP_259498553.1 460024..462108(+) (pilQ) [Pseudomonas sp. N3-W]
ATGAACAGGATTTTCTCGACCCTCGGTCTTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTGCAGGCGGCCAGCCTGAA
TTCGCTGGACGTCGCCGCGCTGCCGGGCGACCGCATTGAGCTCAAGTTGTCGTTCGACGCGCCACCACCACCGCCCAAGG
GCTACACCACCGAGTCACCGGCGCGAATCGCCCTGGACCTGCCCGGCGTGGCCAACAAACTCACCAACAAGAATCGCGAC
CTGGGCCGTGGCAATGCCCGCAGCGCCACGGTGGTGGAGGCCAATGACCGTACGCGGCTGATTATCAGCCTGACGCAGTT
GGCGCCCTACAGCACTCGGGTTGAAGGCAATAATCTGTTTGTCGTGGTCGGACAAGGCGCCAAAGTCAATGTGCCCAAGC
CGGTCGCCAGCGCACCCCGTGCCGCCACGGTAAAACCGGCAGCTGGAAAAACCTGGGCGCCAGTGACCAAGGCGATCCGT
GGCGTGGATTTCCAGCGCGGAACGCAGGGTGAGGGCAATGTGGTCATCGATTTGTCAGATCCATCTATCGCTCCGGACAT
TCAGGAGCGCGACGGCAAGATCATCCTCGGTTTCGCCAGGACCCAACTGCCCGAGCCATTGCGCGTGCGGCTGGACGTCA
AGGACTTCGCCACCCCGGTGCAGTTCGTCAACGCCGCCGCCACTGGCGATCGCGCCACCATCAGCATTGAGCCGGTCGGT
GCCTACGAGTATTCGACCTACCAGACCGACAACAAACTGACCGTCAGCATCCGCGCGGTGACGGCCGATGACTTGCAAAA
GCGCAATGCCGAACGCTTTGCCTACACCGGTGAAAAGCTCTCGCTGAATTTTCAGGACATCGACGTGCGCTCGGTGCTGC
AACTGATCGCCGATTTCACCAACCTCAATCTGGTCGCCAGCGACACGGTGCAGGGAGGCATTACCTTGCGCTTGCAGAAT
GTGCCGTGGGATCAGGCGTTGGACCTGGTGCTGAAAACCAAAGGCCTGGACAAGCGCAAGATCGGCAATGTGTTGCTGGT
GGCGCCGGCCGATGAAATTGCCGCCCGAGAACGTCAGGAACTGGAGTCGCAAAAACAGATCGCCGAATTGGCGCCGCTGC
GTCGCGAGTTGCTGCAGGTCAACTACGCCAAGGCGAAGGACATCGCTGAGCTGTTCAGGTCAGTGACCAACAAGAAACAG
TCGGAAACGGATGAGCGGGGCTCGATCACGGTCGATGAGCGCACCAACAACATCATTGCCTACCAGACTCAGGAGCGCCT
CGACGAGCTGCGCCGCATCGTCGCGCAACTGGATATTCCGGTGCGTCAGGTGATGATCGAAGCGCGCATCGTCGAAGCCA
ACGTCGATTACGACAAGAGCCTCGGCGTGCGCTGGGGCGGCTCGATCCAGAACAAGGGCAACTGGAACACCTCGGGTGTC
ACCAATGGTTCTACCACCACCATCGGTACGCCGGGCAGTACCAGCACCAACTCGCCGTTCGTCGACATGGGGACTGCCGG
CAACACGTCCGGGATTGGTATTGCTTTCATCACCGACAACGTCCTGCTGGACCTCGAACTGACGGCCATGGAGAAAACCG
GCAACGGCGAAATCGTCTCGCAGCCCAAGGTGGTCACGTCCGACAAGGAAACCGCAAAAATCCTCAAGGGCACGGAAATT
CCCTATCAGGAGGCCAGTTCCAGTGGTGCCACCTCGGTGGCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGTGACGCCGCA
GATCACCCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAACCCGACTACCTCAACAAGGTGCAGGACG
TGCCGCCTATCAAGAAAAACGAAGTCAACGCCAAGGTGCTGGTCAATGACGGGGAGACCATCGTGATTGGTGGGGTTTTC
TCCAATACTCAGAGCAAGATTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGA
CGTGGTTTCGGAACAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAATCAGGCGATTGCTGTGAGTC
ATTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(377-443)

AMIN domain (AMIN)

(37-122)

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II and III secretion system protein (Secretin)

(529-685)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.028

100

0.742
  comQ Acinetobacter baylyi ADP1

41.558

99.856

0.415
  pilQ Acinetobacter baumannii strain A118

38.133

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.133

100

0.412
  pilQ Legionella pneumophila strain ERS1305867

37.906

97.695

0.37
  pilQ Legionella pneumophila strain Lp02

37.906

97.695

0.37