Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NPS81_RS16600 Genome accession   NZ_CP103954
Coordinates   3818937..3820673 (-) Length   578 a.a.
NCBI ID   WP_029828360.1    Uniprot ID   -
Organism   Xanthomonas citri pv. glycines strain B1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3813937..3825673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPS81_RS16590 (NPS81_16620) - 3814758..3816371 (+) 1614 WP_016850708.1 HAMP domain-containing sensor histidine kinase -
  NPS81_RS16595 (NPS81_16625) pilR 3816636..3818090 (+) 1455 WP_016850709.1 sigma-54 dependent transcriptional regulator Regulator
  NPS81_RS16600 (NPS81_16630) pilB 3818937..3820673 (-) 1737 WP_029828360.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NPS81_RS16605 (NPS81_16635) pilA/pilAI 3820808..3821215 (-) 408 WP_016849608.1 pilin Machinery gene
  NPS81_RS16610 (NPS81_16640) pilC 3821574..3822827 (+) 1254 WP_026006934.1 type II secretion system F family protein Machinery gene
  NPS81_RS16615 (NPS81_16645) - 3822834..3823697 (+) 864 WP_016849610.1 A24 family peptidase -
  NPS81_RS16620 (NPS81_16650) coaE 3823711..3824322 (+) 612 WP_016849611.1 dephospho-CoA kinase -
  NPS81_RS16625 (NPS81_16655) - 3824717..3824983 (+) 267 WP_154666337.1 hypothetical protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62578.73 Da        Isoelectric Point: 5.3168

>NTDB_id=726605 NPS81_RS16600 WP_029828360.1 3818937..3820673(-) (pilB) [Xanthomonas citri pv. glycines strain B1]
MSVMGTANLVGITGIARRLVQDGAIDESAARAAMDQAAAAKIPLPQWFSEKKLVTASQLAAANAVEFGMPLLDVSVFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGASLG
DDDEGMDNLDVSVGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGDAMQIAEAAQAIGIRDLRQSALM
KAANGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=726605 NPS81_RS16600 WP_029828360.1 3818937..3820673(-) (pilB) [Xanthomonas citri pv. glycines strain B1]
ATGAGTGTCATGGGAACGGCTAATCTGGTTGGTATCACTGGAATCGCTCGGCGTCTGGTTCAGGATGGCGCGATCGACGA
GTCCGCTGCCCGCGCTGCAATGGACCAGGCTGCGGCAGCCAAGATTCCCTTGCCGCAATGGTTCTCCGAGAAGAAGCTGG
TCACCGCATCGCAGCTCGCCGCCGCCAACGCGGTCGAGTTCGGCATGCCGCTGCTGGACGTGTCGGTATTCGATGCCAGC
CAGAATGCGGTCAAGCTGGTCAGCGAGGAGTTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCAGGGCGCTGGACGACATCAAGTTCCATACGAACCTGGTGGTCGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGGCGCACGCTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTGGGTGCGTCGCTCGGC
GACGACGACGAGGGGATGGACAACCTGGACGTGTCGGTCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGCCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGGTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAACAGAAG
CTGTTCCTGGAAGCGATCCACAAGCCCTATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACCGCGCTGGGAATTCTCAACGACGAGACGCGCAATATCTCCACCGCGGAGGATCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTCGCCGCGGCTTTGCGCTCGTTCCTGCGACAGGATCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACTGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACATTGCACACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGCATCGCGCCCTACAACATCA
CCTCGTCGGTGACGCTGGTGATCGCGCAGCGTCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCATTGCTGGCCGAAGGATTCACGCCTGCCCAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
TGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAAGGCGGCGATGCGATGCAGATCGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCGTTGATG
AAGGCTGCGAATGGGGTGACTAGCCTGGCCGAGATCAATCGTGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.172

100

0.554
  pilB Acinetobacter baylyi ADP1

55.634

98.27

0.547
  pilB Legionella pneumophila strain ERS1305867

52.373

98.443

0.516
  pilB Vibrio cholerae strain A1552

49.74

99.827

0.497
  pilF Neisseria gonorrhoeae MS11

49.472

98.27

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

46.809

97.578

0.457
  pilB Vibrio campbellii strain DS40M4

45.87

98.443

0.452
  pilB/pilB1 Synechocystis sp. PCC 6803

36.946

100

0.389