Detailed information NTG0072609

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M2494_RS06250
• Genome accession: NZ_AP019411
• Coordinates: 1470340..1471509 (-)
• Length: 389 a.a.
• NCBI ID: WP_017682837.1
• Uniprot ID: A0A0K8M896
• Organism: Pseudomonas syringae pv. actinidiae strain MAFF613020
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1465340..1476509

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M2494_RS06235 (KPSA1B_101269)	-	1465342..1466406 (+)	1065	WP_003383303.1	microcin C ABC transporter permease YejB	-
M2494_RS06240 (KPSA1B_101270)	-	1466406..1467428 (+)	1023	WP_017698995.1	ABC transporter permease	-
M2494_RS06245 (KPSA1B_101271)	-	1467430..1469007 (+)	1578	WP_017699732.1	ABC transporter ATP-binding protein	-
M2494_RS06250 (KPSA1B_101272)	pilU	1470340..1471509 (-)	1170	WP_017682837.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
M2494_RS06255 (KPSA1B_101273)	-	1471664..1471939 (+)	276	WP_003373489.1	peptidylprolyl isomerase	-
M2494_RS06260 (KPSA1B_101274)	ltrA	1472490..1473857 (+)	1368	WP_003373498.1	group II intron reverse transcriptase/maturase	-
M2494_RS06265 (KPSA1B_101275)	pgm	1474265..1475911 (-)	1647	WP_110459642.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42669.81 Da
• Isoelectric Point: 5.8676

>NTDB_id=72609 M2494_RS06250 WP_017682837.1 1470340..1471509(-) (pilU) [Pseudomonas syringae pv. actinidiae strain MAFF613020]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVVAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSENGVTSLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=72609 M2494_RS06250 WP_017682837.1 1470340..1471509(-) (pilU) [Pseudomonas syringae pv. actinidiae strain MAFF613020]
ATGGATTTCCCGGCGTTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTTTATCTTTCTACCGGAGCACCGCCGTG
TGCCAAATTCAACGGCGTGCTCAAACCGCTGGGCAGCGAAACGCTCAAGCCCGGCGAAGTGGCGGTGGTTGCCCAGGGGC
TGATGGACGAGGAACAGAAGCTCGAGTTTCTCCGGGAACTGGAGATGAACCTCGCGGTGTCATTGGCCGGCATTGGTCGG
TTCCGGATCAATATCTTCATGCAGCGCAACGAAGTGTCCATTGTCGCGCGTAACATCAAGCTTGATATTCCGCGCTTTGA
GGACCTGTTCCTGCCGCCGGTATTGCTCGACGTGATCATGGAAAAACACGGTCTGGTGCTGTTTGTCGGCGCCACCGGCT
CGGGCAAATCGACCTCGCTGGCGGCACTGATCGATTACCGTAACCGCAATGCCAGCGGGCATATCATCACCATTGAAGAC
CCGGTCGAATTTATCCATCGGCACAAGAAATCCATCGTCAATCAGCGGGAGGTCGGCGTCGACACCCGCAGCTTTCGCGC
GGCACTGAAAAACACCTTGCGTCAGGCCCCGGATGTGATTCTGATCGGCGAGATTCGCGATCGCGAAACCATGGAGCACG
CGCTGGCATTCGCCGACACCGGCCACCTGGCGATTTCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGAATC
ATCAACTTCTTCCCGGAAGAGCGGCGTGCGCAGCTGTTGCACGACCTTGGCAACAACCTCAAGGCGTTCGTGTCTCAGCG
GCTGGTAAAGACGCCTGATGGAAAACGGCGCGCGGCGGTGGAGGTGATGATGGGCACGCCGACCATCCGCGATCTGATCC
AGCGTAATGAGCTGACTGAACTCAAGGGCATCATGGAAAAGTCCGGGAGCCTCGGGATGCAGACGTTCGATACCGCGCTG
TTCAACCTGGCCGTCGAAGGTGCGATCAGCGAGGAAGAAGCGTTGAAGAATGCCGACTCGCAAAACAACGTGCGCCTTCG
CCTCAAACTGCACAGCGAAAATGGAGTGACCAGCCTGACCACGCCGCCCCCGGCACCGACCGGCAGCAGCACAGCCAGCA
CCGCCGAGTGGGGCCTGGTCGACGATGACGAGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-248)

3D structure

• AlphaFold DB: A0A0K8M896

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.072	99.486	0.558
pilU	Acinetobacter baylyi ADP1	51.831	91.26	0.473
pilU	Vibrio cholerae strain A1552	51.429	89.974	0.463
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.426	98.458	0.388
pilT	Pseudomonas aeruginosa PAK	41.399	88.175	0.365