Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NYR76_RS05010 Genome accession   NZ_CP103821
Coordinates   1118872..1119309 (-) Length   145 a.a.
NCBI ID   WP_279443724.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 3750     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1113872..1124309
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR76_RS04985 (NYR76_04985) yacG 1114776..1114964 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR76_RS04990 (NYR76_04990) coaE 1114946..1115584 (-) 639 WP_279443720.1 dephospho-CoA kinase -
  NYR76_RS04995 (NYR76_04995) - 1115606..1116280 (-) 675 WP_279443721.1 prepilin peptidase -
  NYR76_RS05000 (NYR76_05000) pilC 1116280..1117473 (-) 1194 WP_279443722.1 type II secretion system F family protein Machinery gene
  NYR76_RS05005 (NYR76_05005) pilB 1117466..1118851 (-) 1386 WP_279443723.1 GspE/PulE family protein Machinery gene
  NYR76_RS05010 (NYR76_05010) pilA 1118872..1119309 (-) 438 WP_279443724.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR76_RS05015 (NYR76_05015) radA 1119490..1120869 (+) 1380 WP_279443725.1 DNA repair protein RadA Machinery gene
  NYR76_RS05020 (NYR76_05020) pepB 1120935..1122221 (+) 1287 WP_279443781.1 aminopeptidase PepB -
  NYR76_RS05025 (NYR76_05025) - 1122349..1123284 (+) 936 WP_279443726.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR76_RS05030 (NYR76_05030) greB 1123294..1123782 (+) 489 WP_005611555.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15500.85 Da        Isoelectric Point: 7.5775

>NTDB_id=725542 NYR76_RS05010 WP_279443724.1 1118872..1119309(-) (pilA) [Actinobacillus equuli subsp. equuli strain 3750]
MKFVFKPIQQAFTLIELMIVIAIIAILATVAIPSYNNYTKKAALSELLAASSAYKTDVEICIYNTGEVKNCSSGTNGIKN
GDDIKNSKYLASISVASGVITVKGKGSVDGYGYTLTPDYSNKTLTWETACLGEDTSLFPAAFCSK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=725542 NYR76_RS05010 WP_279443724.1 1118872..1119309(-) (pilA) [Actinobacillus equuli subsp. equuli strain 3750]
ATGAAATTCGTATTCAAGCCTATACAGCAGGCATTTACCCTGATTGAATTAATGATTGTAATTGCAATCATTGCTATTTT
GGCAACGGTAGCCATTCCTTCTTATAACAATTACACCAAAAAGGCGGCATTATCCGAACTGTTAGCGGCATCTTCCGCTT
ATAAAACAGATGTGGAAATTTGTATTTATAACACGGGAGAGGTAAAAAACTGTTCTAGCGGAACGAATGGCATCAAAAAT
GGTGACGATATTAAAAACTCTAAGTATTTAGCCTCTATTTCTGTAGCTTCCGGTGTAATAACTGTGAAGGGGAAAGGGAG
TGTAGATGGGTATGGATATACGCTTACACCGGATTATTCAAATAAAACGCTAACTTGGGAAACGGCTTGTTTAGGCGAGG
ATACTAGTCTATTCCCTGCCGCGTTTTGTTCGAAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-128)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

65.517

100

0.655
  pilA Haemophilus influenzae Rd KW20

56.738

97.241

0.552
  pilA Haemophilus influenzae 86-028NP

56.429

96.552

0.545
  pilA Vibrio campbellii strain DS40M4

37.589

97.241

0.366