NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	NYR48_RS12695	Genome accession	NZ_CP103782
Coordinates	2605680..2606294 (-)	Length	204 a.a.
NCBI ID	WP_259424289.1	Uniprot ID	-
Organism	Bacillus velezensis strain DA4
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2600680..2611294

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NYR48_RS12665	rpsT	2600983..2601249 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
NYR48_RS12670	holA	2601266..2602307 (-)	1042	Protein_2450	DNA polymerase III subunit delta	-
NYR48_RS12675	-	2602347..2602498 (-)	152	Protein_2451	hypothetical protein	-
NYR48_RS12680	-	2602539..2602673 (+)	135	WP_003152870.1	YqzM family protein	-
NYR48_RS12685	comEC	2602692..2605043 (-)	2352	WP_259424288.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
NYR48_RS12690	-	2605044..2605613 (-)	570	WP_003152868.1	ComE operon protein 2	-
NYR48_RS12695	comEA	2605680..2606294 (-)	615	WP_259424289.1	helix-hairpin-helix domain-containing protein	Machinery gene
NYR48_RS12700	comER	2606353..2607174 (+)	822	WP_259424290.1	late competence protein ComER	-
NYR48_RS12705	-	2607243..2607980 (-)	738	WP_031378935.1	class I SAM-dependent methyltransferase	-
NYR48_RS12710	rsfS	2607977..2608333 (-)	357	WP_007408260.1	ribosome silencing factor	-
NYR48_RS12715	yqeK	2608351..2608911 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
NYR48_RS12720	-	2608901..2609470 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
NYR48_RS12725	yhbY	2609481..2609771 (-)	291	WP_259424291.1	ribosome assembly RNA-binding protein YhbY	-
NYR48_RS12730	aroE	2609765..2610607 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22210.26 Da Isoelectric Point: 5.0355

                                >NTDB_id=724865 NYR48_RS12695 WP_259424289.1 2605680..2606294(-) (comEA) [Bacillus velezensis strain DA4]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKEDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=724865 NYR48_RS12695 WP_259424289.1 2605680..2606294(-) (comEA) [Bacillus velezensis strain DA4]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCTTCAGCAT

CCGTCGCGGACAAAGAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAACTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG

CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGA

CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363

Detailed information NTG0724865