Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   PYW37_RS02560 Genome accession   NZ_CP118969
Coordinates   478513..480396 (+) Length   627 a.a.
NCBI ID   WP_025017100.1    Uniprot ID   -
Organism   Lactococcus lactis strain DSM 20481     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 473513..485396
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYW37_RS02525 - 473875..474111 (+) 237 WP_023189749.1 hypothetical protein -
  PYW37_RS02530 - 474124..474348 (+) 225 WP_023189750.1 hemolysin XhlA family protein -
  PYW37_RS02535 - 474361..474648 (+) 288 WP_010905390.1 phage holin -
  PYW37_RS02540 - 474648..475427 (+) 780 WP_058222357.1 peptidoglycan amidohydrolase family protein -
  PYW37_RS02550 rsmB 476382..477656 (+) 1275 WP_014570715.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  PYW37_RS02555 - 477737..478513 (+) 777 WP_025017099.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  PYW37_RS02560 pknB 478513..480396 (+) 1884 WP_025017100.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  PYW37_RS02565 rpsO 480578..480847 (+) 270 WP_010906184.1 30S ribosomal protein S15 -
  PYW37_RS02570 proC 480878..481666 (-) 789 WP_012898440.1 pyrroline-5-carboxylate reductase -
  PYW37_RS02575 glmU 481894..483270 (+) 1377 WP_025017101.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
  PYW37_RS02580 - 483375..483959 (+) 585 WP_012898439.1 NUDIX hydrolase -
  PYW37_RS02585 macP 483952..484230 (+) 279 WP_003130796.1 cell wall synthase accessory phosphoprotein MacP -
  PYW37_RS02590 - 484263..484943 (+) 681 WP_023189755.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -

Sequence

Protein


Download         Length: 627 a.a.        Molecular weight: 67973.06 Da        Isoelectric Point: 4.7401

>NTDB_id=724198 PYW37_RS02560 WP_025017100.1 478513..480396(+) (pknB) [Lactococcus lactis strain DSM 20481]
MIQIGKIFADRYRIIKEIGRGGMANVYQGEDTFLGDRLVAIKVLRSNFENDDIAIARFQREAFAMAELSHPNIVGISDVG
EFESQQYIVMEFVDGMTLKQYINQNAPLANDEAIEIITEILSAMDMAHSHGIIHRDLKPQNVLVSSSGTVKVTDFGIAKA
LSETSLTQTNTMFGSVHYLSPERTRGSNATVQSDIYAIGIILFELLTGQIPFDGDSAVAIALKHFQESIPSIINLNPEVP
QALENVVIKATAKDIKNRYTDVEEMMTDVATSTSLDRRGEEKLVFNKDHDETKIMPANLINPYDTKPLIDKKEDNDSQTD
EKAASSEVVNKNKKSKKGLIIGLIILLLVVGGATLAWVVSTPTNVKIPNVTNSTLDQAKSKIKDAKLKVGTVHKQQSSTI
AEGNVIKTDPTSGTTVRSNSSVDIYVSTGNEDIIKMKDFVGEKIDEAMATLLKDYGIDESQVTQTSVPSDSYPAGTIIKQ
SPKKGSSFDTKGSEKITFEVSSGKQVEVPDYKPNGQYMTYSQYQAALKAAGFTNITLDPQATTNQQADGYVYSVYPTVGA
SVDPTQEIVITYSVYTAPSSSSTTSESTTTPETSSSTTSSTSSSTTSQPSTDNNNSSKESSTTSSSS

Nucleotide


Download         Length: 1884 bp        

>NTDB_id=724198 PYW37_RS02560 WP_025017100.1 478513..480396(+) (pknB) [Lactococcus lactis strain DSM 20481]
ATGATTCAAATCGGTAAAATCTTTGCCGATCGTTATCGGATTATCAAAGAAATCGGACGTGGCGGAATGGCGAACGTTTA
TCAAGGTGAAGATACTTTTCTTGGTGACCGCTTAGTTGCAATAAAAGTGCTACGTTCAAATTTTGAAAACGATGATATCG
CGATTGCTCGTTTTCAGCGAGAAGCATTCGCCATGGCAGAACTTTCACATCCAAATATCGTAGGTATTTCTGATGTTGGT
GAATTTGAAAGTCAACAATACATTGTCATGGAATTTGTTGACGGCATGACACTTAAACAATACATCAATCAAAATGCACC
TTTAGCCAATGATGAAGCTATTGAAATTATTACTGAAATTTTATCAGCAATGGACATGGCCCATTCACATGGTATTATTC
ACCGGGATTTAAAACCACAAAATGTTTTAGTTTCCAGTAGTGGAACTGTAAAAGTAACGGATTTTGGAATTGCGAAAGCA
CTCTCTGAAACTTCACTTACTCAAACAAATACAATGTTTGGTTCGGTTCATTATTTATCACCAGAGCGAACGCGTGGTTC
AAATGCAACTGTTCAATCAGATATTTATGCGATTGGAATTATTTTATTTGAACTTTTAACAGGTCAAATTCCATTTGATG
GAGATTCTGCAGTTGCTATCGCTCTAAAACATTTCCAAGAGAGTATTCCAAGCATCATCAATCTCAATCCAGAAGTTCCG
CAAGCTTTGGAAAATGTTGTCATTAAAGCTACAGCGAAAGATATTAAAAATCGCTATACTGATGTAGAAGAAATGATGAC
TGATGTGGCAACTTCAACGAGTCTTGATCGTCGTGGGGAAGAAAAATTAGTTTTCAATAAGGACCATGATGAAACAAAAA
TTATGCCTGCCAATCTAATTAATCCTTATGACACCAAGCCTCTGATTGATAAAAAAGAAGATAATGATTCACAAACAGAT
GAAAAAGCTGCAAGCTCAGAAGTAGTAAATAAAAACAAAAAATCTAAAAAAGGCTTGATTATTGGTCTTATTATCCTTCT
TTTAGTTGTTGGCGGTGCTACTTTGGCTTGGGTTGTCTCAACACCAACTAATGTTAAGATACCTAATGTGACAAACTCGA
CGCTTGACCAAGCAAAAAGCAAAATTAAAGATGCAAAACTAAAAGTGGGAACAGTTCATAAACAACAAAGTTCAACGATT
GCTGAAGGAAATGTTATCAAGACTGATCCAACTAGTGGAACAACCGTGAGATCTAATAGCTCCGTTGATATTTATGTTTC
TACGGGAAATGAAGATATCATCAAAATGAAGGACTTTGTCGGTGAAAAGATTGATGAGGCGATGGCAACATTACTTAAGG
ATTATGGTATTGATGAAAGCCAAGTCACTCAAACTTCCGTTCCTAGTGATTCTTACCCAGCAGGTACAATCATTAAACAA
AGCCCTAAAAAAGGTTCTAGTTTTGATACCAAAGGGAGTGAAAAAATTACCTTTGAAGTTTCAAGTGGAAAACAAGTTGA
AGTTCCGGATTATAAACCGAACGGTCAATATATGACTTATTCACAGTATCAAGCTGCCTTAAAAGCTGCAGGATTCACTA
ATATTACACTAGACCCTCAAGCAACAACGAATCAACAAGCCGACGGTTATGTATATTCTGTTTATCCGACTGTTGGAGCG
TCCGTTGATCCAACTCAAGAAATTGTAATCACTTATAGCGTCTATACTGCTCCAAGTTCAAGTTCAACAACTTCTGAATC
AACAACGACGCCTGAAACTTCGTCAAGCACAACTTCAAGTACTTCAAGTTCTACAACAAGTCAGCCGTCAACAGATAACA
ACAATAGCAGCAAAGAATCTTCAACAACGTCATCAAGTAGTTGA

Domains

Predicted by InterProScan.

Protein kinase domain (Pkinase)

(12-265)

PASTA domain (PASTA)

(506-573)

PASTA domain (PASTA)

(365-428)

PASTA domain (PASTA)

(434-501)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

48.529

86.762

0.421
  stkP/pknB Streptococcus salivarius strain HSISS4

50.674

82.775

0.419
  stkP Streptococcus pneumoniae TIGR4

47.628

80.702

0.384
  stkP Streptococcus pneumoniae D39

47.431

80.702

0.383
  stkP Streptococcus pneumoniae R6

47.431

80.702

0.383
  stkP Streptococcus pneumoniae Rx1

47.233

80.702

0.381