Detailed information NTG0723514

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: PWP91_RS00345
• Genome accession: NZ_CP118738
• Coordinates: 71266..72267 (-)
• Length: 333 a.a.
• NCBI ID: WP_003131994.1
• Uniprot ID: Q9CDL3
• Organism: Lactococcus lactis strain P-1
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 66266..77267

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PWP91_RS00310 (PWP91_00310)	-	66554..66919 (-)	366	WP_003131985.1	MerR family transcriptional regulator	-
PWP91_RS00315 (PWP91_00315)	-	67020..67457 (-)	438	WP_003131986.1	low molecular weight protein-tyrosine-phosphatase	-
PWP91_RS00320 (PWP91_00320)	-	67601..67765 (-)	165	WP_003131988.1	hypothetical protein	-
PWP91_RS00325 (PWP91_00325)	-	67870..68487 (-)	618	WP_012898695.1	AAA family ATPase	-
PWP91_RS00330 (PWP91_00330)	-	68484..69668 (-)	1185	WP_014570851.1	ABC transporter permease	-
PWP91_RS00335 (PWP91_00335)	-	69735..70019 (-)	285	WP_274998666.1	hypothetical protein	-
PWP91_RS00340 (PWP91_00340)	-	70245..71129 (-)	885	WP_058203823.1	XRE/MutR family transcriptional regulator	-
PWP91_RS00345 (PWP91_00345)	ruvB	71266..72267 (-)	1002	WP_003131994.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
PWP91_RS00350 (PWP91_00350)	ruvA	72394..72987 (-)	594	WP_003131995.1	Holliday junction branch migration protein RuvA	Machinery gene
PWP91_RS00355 (PWP91_00355)	hexB	73100..75070 (-)	1971	WP_058204906.1	DNA mismatch repair endonuclease MutL	Machinery gene
PWP91_RS00360 (PWP91_00360)	-	75200..75895 (-)	696	WP_274998667.1	hypothetical protein	-

Sequence

Protein

• Length: 333 a.a.
• Molecular weight: 37633.17 Da
• Isoelectric Point: 4.5825

>NTDB_id=723514 PWP91_RS00345 WP_003131994.1 71266..72267(-) (ruvB) [Lactococcus lactis strain P-1]
MNDILNKEPMEEDYGIEKSLRPQFFNQYIGQDKVKEQLEIFIKAAKMREEVLDHVLLFGPPGLGKTTMAFVIANELGVNI
KQTAGPAIEKPGDLVAILNELEPGDVLFIDEIHRMPMQVEEVLYSAMEDFYIDIMLGSGDGSRSVHLDLPPFTLVGATTR
AGMLSNPLRARFGISSHMEYYQERDLEEIVKRTADIFEVEVIDNAALEIALRSRGTPRIANRLLKRVRDFAQIMGDGRVD
KAITDKALSILDVDAAGLDYIDQKILRTMIEMYHGGPVGIGTLAVNIAEDRETVEDMYEPYLIQKGFLMRTKQGRKVTQR
AYEHLGYVYNEED

Nucleotide

• Length: 1002 bp

>NTDB_id=723514 PWP91_RS00345 WP_003131994.1 71266..72267(-) (ruvB) [Lactococcus lactis strain P-1]
ATGAACGATATATTAAACAAAGAGCCAATGGAAGAAGATTACGGTATTGAAAAATCTCTACGTCCGCAATTTTTTAATCA
ATATATTGGTCAAGATAAAGTCAAAGAACAATTAGAAATCTTTATTAAAGCAGCAAAAATGCGTGAAGAAGTTTTGGACC
ATGTATTACTTTTTGGTCCTCCGGGACTTGGTAAGACAACAATGGCATTTGTCATTGCCAATGAGTTGGGGGTTAATATT
AAACAAACAGCGGGTCCTGCCATTGAAAAGCCAGGAGATTTAGTTGCGATTTTGAATGAATTGGAACCTGGCGATGTGCT
ATTTATTGATGAAATTCATCGGATGCCAATGCAAGTTGAAGAAGTTCTTTATAGCGCTATGGAAGATTTCTACATTGATA
TTATGCTTGGTTCTGGTGATGGTTCACGCTCGGTTCATTTAGATTTGCCGCCATTTACTCTAGTTGGGGCAACAACAAGA
GCTGGAATGCTTTCGAATCCATTGCGTGCCCGTTTTGGAATTTCAAGTCATATGGAGTATTATCAAGAGCGTGATTTGGA
AGAAATTGTTAAAAGAACAGCTGATATTTTTGAAGTCGAAGTTATTGATAATGCCGCTTTAGAGATTGCTTTACGTAGTC
GAGGGACTCCGCGGATTGCCAATCGTTTACTTAAACGAGTGCGTGATTTTGCTCAAATAATGGGGGATGGCCGAGTGGAT
AAAGCAATTACCGATAAGGCCTTGTCTATTCTTGATGTTGATGCCGCTGGTTTGGACTATATTGACCAAAAAATTCTCCG
CACAATGATTGAAATGTACCACGGTGGTCCTGTTGGGATTGGAACTTTGGCGGTAAATATTGCCGAAGACCGTGAAACGG
TCGAGGATATGTATGAACCTTATTTGATTCAAAAAGGATTTTTGATGAGAACTAAACAGGGACGTAAGGTGACCCAAAGA
GCCTATGAACACCTAGGTTATGTATACAATGAAGAAGATTGA

Domains

Predicted by InterProScan.

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: Q9CDL3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	78.614	99.7	0.784
ruvB	Streptococcus pneumoniae D39	78.614	99.7	0.784
ruvB	Streptococcus pneumoniae TIGR4	78.313	99.7	0.781
ruvB	Bacillus subtilis subsp. subtilis str. 168	61.491	96.697	0.595
ruvB	Helicobacter pylori 26695	51.242	96.697	0.495
ruvB	Synechocystis sp. PCC 6803	51.613	93.093	0.48