Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NX720_RS15470 Genome accession   NZ_CP103300
Coordinates   3761134..3762840 (-) Length   568 a.a.
NCBI ID   WP_262595702.1    Uniprot ID   -
Organism   Endozoicomonas euniceicola strain EF212     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 3725088..3765098 3761134..3762840 within 0


Gene organization within MGE regions


Location: 3725088..3765098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX720_RS15210 (NX720_15210) - 3725088..3726089 (-) 1002 WP_262595647.1 contractile injection system protein, VgrG/Pvc8 family -
  NX720_RS15215 (NX720_15215) - 3726080..3726289 (-) 210 WP_262595648.1 tail protein X -
  NX720_RS15220 (NX720_15220) - 3726258..3726665 (-) 408 WP_262595649.1 phage tail protein -
  NX720_RS15225 (NX720_15225) - 3726667..3728619 (-) 1953 WP_262595650.1 guided entry of tail-anchored proteins factor 1 -
  NX720_RS15230 (NX720_15230) - 3728753..3728992 (-) 240 WP_262595651.1 phage tail assembly protein -
  NX720_RS15235 (NX720_15235) - 3729007..3729516 (-) 510 WP_262595652.1 phage major tail tube protein -
  NX720_RS15240 (NX720_15240) - 3729513..3730664 (-) 1152 WP_262595653.1 phage tail sheath subtilisin-like domain-containing protein -
  NX720_RS15245 (NX720_15245) - 3730761..3731258 (-) 498 WP_262595654.1 DUF4376 domain-containing protein -
  NX720_RS15250 (NX720_15250) - 3731261..3732517 (-) 1257 WP_262595655.1 gp53-like domain-containing protein -
  NX720_RS15255 (NX720_15255) - 3732514..3733572 (-) 1059 WP_262595656.1 phage tail protein I -
  NX720_RS15260 (NX720_15260) - 3733565..3734467 (-) 903 WP_262595657.1 baseplate J/gp47 family protein -
  NX720_RS15265 (NX720_15265) - 3734467..3734802 (-) 336 WP_262595658.1 GPW/gp25 family protein -
  NX720_RS15270 (NX720_15270) - 3734854..3735432 (-) 579 WP_262595659.1 phage baseplate assembly protein V -
  NX720_RS15275 (NX720_15275) - 3735432..3735968 (-) 537 WP_262595660.1 hypothetical protein -
  NX720_RS15280 (NX720_15280) - 3735938..3736555 (-) 618 WP_262595661.1 hypothetical protein -
  NX720_RS15285 (NX720_15285) - 3736548..3736853 (-) 306 WP_262595662.1 head-tail joining protein -
  NX720_RS15290 (NX720_15290) - 3736868..3737131 (-) 264 WP_262595664.1 hypothetical protein -
  NX720_RS15295 (NX720_15295) - 3737112..3737357 (-) 246 WP_262595665.1 hypothetical protein -
  NX720_RS15300 (NX720_15300) - 3737354..3738346 (-) 993 WP_262595666.1 hypothetical protein -
  NX720_RS15305 (NX720_15305) - 3738343..3738555 (-) 213 WP_262595667.1 hypothetical protein -
  NX720_RS15310 (NX720_15310) - 3738642..3740564 (-) 1923 WP_262595668.1 phage major capsid protein -
  NX720_RS15315 (NX720_15315) - 3740512..3742053 (-) 1542 WP_262595669.1 phage portal protein -
  NX720_RS15320 (NX720_15320) - 3742053..3742583 (-) 531 WP_262595671.1 hypothetical protein -
  NX720_RS15325 (NX720_15325) - 3742590..3744524 (-) 1935 WP_262595672.1 phage terminase large subunit family protein -
  NX720_RS15330 (NX720_15330) - 3744496..3744972 (-) 477 WP_262595673.1 terminase small subunit -
  NX720_RS15335 (NX720_15335) - 3745080..3745634 (-) 555 WP_262595674.1 hypothetical protein -
  NX720_RS15340 (NX720_15340) - 3745636..3746025 (-) 390 WP_262595675.1 D-Ala-D-Ala carboxypeptidase family metallohydrolase -
  NX720_RS15345 (NX720_15345) - 3746025..3746414 (-) 390 WP_262595676.1 phage holin family protein -
  NX720_RS15350 (NX720_15350) - 3746411..3746794 (-) 384 WP_262595677.1 hypothetical protein -
  NX720_RS15355 (NX720_15355) - 3746806..3747192 (-) 387 WP_262595678.1 RusA family crossover junction endodeoxyribonuclease -
  NX720_RS15360 (NX720_15360) - 3747433..3749427 (-) 1995 WP_262595679.1 AAA family ATPase -
  NX720_RS27185 - 3749522..3749722 (-) 201 WP_404830999.1 transcriptional regulator -
  NX720_RS15365 (NX720_15365) - 3749931..3750581 (+) 651 WP_262595681.1 LexA family transcriptional regulator -
  NX720_RS15370 (NX720_15370) - 3750745..3751014 (+) 270 WP_262595682.1 hypothetical protein -
  NX720_RS15375 (NX720_15375) - 3751063..3751545 (+) 483 WP_262595683.1 hypothetical protein -
  NX720_RS15380 (NX720_15380) - 3751590..3752009 (+) 420 WP_262595684.1 hypothetical protein -
  NX720_RS15385 (NX720_15385) - 3752009..3752794 (+) 786 WP_262595685.1 ATP-binding protein -
  NX720_RS15390 (NX720_15390) - 3752797..3753405 (+) 609 WP_262595687.1 DUF669 domain-containing protein -
  NX720_RS15395 (NX720_15395) - 3753466..3754515 (+) 1050 WP_262595688.1 PD-(D/E)XK nuclease family protein -
  NX720_RS15400 (NX720_15400) - 3754538..3754720 (+) 183 WP_262595689.1 hypothetical protein -
  NX720_RS15405 (NX720_15405) - 3754746..3755102 (+) 357 WP_262595690.1 hypothetical protein -
  NX720_RS15410 (NX720_15410) - 3755096..3755299 (+) 204 WP_262595691.1 hypothetical protein -
  NX720_RS15415 (NX720_15415) - 3755289..3757097 (+) 1809 WP_262595692.1 DEAD/DEAH box helicase -
  NX720_RS15420 (NX720_15420) - 3757108..3757257 (+) 150 WP_262595693.1 hypothetical protein -
  NX720_RS15425 (NX720_15425) - 3757250..3757453 (+) 204 WP_262595694.1 hypothetical protein -
  NX720_RS15430 (NX720_15430) - 3757462..3757734 (+) 273 WP_262595695.1 hypothetical protein -
  NX720_RS15435 (NX720_15435) - 3757719..3758084 (+) 366 WP_262595696.1 hypothetical protein -
  NX720_RS15440 (NX720_15440) - 3758425..3758625 (+) 201 WP_262595697.1 hypothetical protein -
  NX720_RS15445 (NX720_15445) - 3758622..3759059 (+) 438 WP_262595698.1 DUF3268 family zinc-finger domain-containing protein -
  NX720_RS15450 (NX720_15450) - 3759080..3759346 (+) 267 WP_262595699.1 hypothetical protein -
  NX720_RS15455 (NX720_15455) - 3759470..3759745 (+) 276 WP_262595700.1 hypothetical protein -
  NX720_RS15460 (NX720_15460) - 3759735..3760790 (-) 1056 WP_262595701.1 tyrosine-type recombinase/integrase -
  NX720_RS15470 (NX720_15470) pilB 3761134..3762840 (-) 1707 WP_262595702.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NX720_RS15475 (NX720_15475) - 3763015..3764319 (-) 1305 WP_262595703.1 MATE family efflux transporter -
  NX720_RS15480 (NX720_15480) - 3764316..3765098 (-) 783 WP_262595704.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62622.35 Da        Isoelectric Point: 4.6315

>NTDB_id=721820 NX720_RS15470 WP_262595702.1 3761134..3762840(-) (pilB) [Endozoicomonas euniceicola strain EF212]
MADTPLNGLAARLVVNQLLDEGAVRSAQAKAEQSSKTLSQQLVEDKQLTYPQLALVTAEEFGIPVFDLTAFDPDNIPRDL
INENLIRKHKVMPLIKRGTRLFVAVVNPADHQAIDEIRFNTGLAAEPVLVEENKIEAFIDRVIEGGDNSLNDFDDDEFEN
LDDLEVSAVEDAAEDYSGSAEDDAPVVKFVNKMLLSAIKGGASDLHFEPYEKSYRVRFRTDGVLHEVARPPATLSPRIAS
RLKIMSAMDISERRKPQDGRIKMKLSKSKAIDFRVNTLPTLWGEKIVLRILDPSSAKMGIDALGYEEEQKAMYLEALEQP
QGMILVTGPTGSGKTVSLYTGLNILNTPERNISTSEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGEIR
DLETANIAIKAAQTGHMVMSTLHTNSAPETLTRLQNMGVPSFNIATSVSLIIAQRLARRLCTNCKEEVDIPKETLLEEGF
TEEQAKEAVIYGPKGCEQCSKGYKGRVGIYEVVKITPAMQQVIMAEGNSLEIADVARKEGFSNLRQSGLLKALQGVTSLE
EVNRVTLD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=721820 NX720_RS15470 WP_262595702.1 3761134..3762840(-) (pilB) [Endozoicomonas euniceicola strain EF212]
ATGGCAGACACCCCCCTGAACGGTCTGGCCGCGCGCCTGGTGGTCAATCAGCTGCTTGATGAAGGTGCGGTACGATCTGC
TCAGGCTAAAGCAGAGCAGTCCAGCAAAACCCTGTCTCAGCAACTGGTTGAAGACAAACAGCTGACCTACCCACAGCTGG
CACTGGTGACCGCTGAAGAGTTCGGCATTCCTGTGTTCGACCTTACCGCTTTCGATCCTGACAATATTCCAAGAGACCTC
ATTAACGAAAACCTCATCCGCAAGCACAAGGTAATGCCTTTAATTAAACGCGGAACCCGCCTGTTTGTTGCCGTCGTCAA
TCCAGCCGACCATCAGGCTATTGATGAAATTCGCTTCAACACAGGTCTTGCTGCCGAGCCTGTTCTGGTTGAAGAAAACA
AAATTGAAGCATTCATTGACCGGGTTATAGAGGGTGGTGATAACTCGCTCAATGACTTTGATGATGACGAATTTGAGAAC
CTTGATGACCTGGAAGTCAGTGCTGTCGAAGACGCAGCCGAGGATTACAGTGGTTCGGCAGAAGACGATGCCCCGGTGGT
AAAATTTGTGAACAAAATGCTGCTGAGTGCTATTAAGGGAGGCGCTTCCGATCTGCACTTCGAACCTTACGAAAAAAGTT
ACCGGGTGCGTTTTCGAACAGATGGTGTTCTGCACGAAGTCGCCAGGCCACCCGCTACACTCTCTCCAAGAATTGCCTCC
CGGCTGAAAATTATGTCGGCCATGGATATTTCTGAGCGTCGCAAACCTCAGGATGGCCGCATAAAAATGAAGCTGTCGAA
AAGCAAGGCGATTGATTTCCGGGTCAACACACTCCCAACCCTTTGGGGGGAGAAGATCGTACTCCGTATTCTGGACCCGT
CCAGTGCAAAAATGGGGATTGACGCACTGGGTTACGAAGAAGAGCAGAAAGCCATGTACCTTGAAGCCCTGGAGCAGCCA
CAGGGCATGATTCTGGTCACAGGCCCTACAGGGTCAGGTAAAACCGTATCGCTTTACACCGGCCTGAATATTCTGAACAC
CCCGGAACGCAATATCTCTACCTCAGAAGACCCGGTGGAAATCAACCTTGAAGGCATTAACCAGGTCAACGTAAATCCCA
AGCAGGGCATGGATTTCTCTCAGGCGCTCAGGGCATTTCTACGACAGGATCCTGATATCATCATGGTGGGTGAGATCCGG
GACCTGGAAACCGCCAACATCGCCATCAAAGCAGCCCAGACGGGTCACATGGTGATGTCAACGCTGCACACCAACAGTGC
GCCGGAAACCCTGACCCGTCTGCAGAATATGGGCGTTCCGTCGTTCAACATAGCCACCTCCGTCAGTCTTATCATCGCCC
AGCGCCTGGCAAGACGGCTTTGCACCAACTGCAAAGAGGAAGTCGACATTCCCAAAGAGACATTGCTGGAAGAAGGCTTT
ACCGAAGAACAGGCCAAAGAGGCCGTTATTTATGGACCGAAAGGCTGCGAGCAGTGCAGCAAGGGCTATAAAGGCCGGGT
TGGTATTTATGAAGTGGTGAAAATTACGCCAGCCATGCAGCAGGTCATTATGGCAGAAGGCAACTCCCTGGAGATTGCGG
ATGTGGCCAGAAAAGAAGGTTTTAGTAATCTCCGACAATCCGGTTTGCTGAAAGCGCTGCAAGGGGTCACCAGCCTGGAA
GAAGTTAACCGGGTAACGCTGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.367

99.648

0.621
  pilB Acinetobacter baylyi ADP1

59.325

99.12

0.588
  pilB Legionella pneumophila strain ERS1305867

55.477

99.648

0.553
  pilF Neisseria gonorrhoeae MS11

49.206

99.824

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

50.276

95.599

0.481
  pilB Vibrio cholerae strain A1552

52.734

90.141

0.475
  pilB Vibrio campbellii strain DS40M4

51.765

89.789

0.465
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.659

96.127

0.391
  pilF Thermus thermophilus HB27

39.312

97.183

0.382