Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NUW46_RS13480 Genome accession   NZ_CP103058
Coordinates   2915738..2917453 (+) Length   571 a.a.
NCBI ID   WP_418140351.1    Uniprot ID   -
Organism   Marinobacter sp. MA     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2891285..2914765 2915738..2917453 flank 973


Gene organization within MGE regions


Location: 2891285..2917453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUW46_RS13375 (NUW46_13400) - 2891285..2891998 (-) 714 WP_418140329.1 hypothetical protein -
  NUW46_RS13380 (NUW46_13405) - 2892275..2893537 (-) 1263 WP_418140330.1 hypothetical protein -
  NUW46_RS13385 (NUW46_13410) - 2893644..2894672 (-) 1029 WP_418140332.1 glycosyltransferase family 2 protein -
  NUW46_RS13390 (NUW46_13415) - 2894741..2896186 (-) 1446 WP_418140333.1 lipopolysaccharide biosynthesis protein -
  NUW46_RS13395 (NUW46_13420) - 2896271..2897245 (-) 975 WP_418140334.1 sulfotransferase -
  NUW46_RS13400 (NUW46_13425) - 2897278..2898288 (-) 1011 WP_418140335.1 sulfotransferase -
  NUW46_RS13405 (NUW46_13430) - 2898291..2898980 (-) 690 WP_418140336.1 WbqC family protein -
  NUW46_RS13410 (NUW46_13435) - 2898980..2900077 (-) 1098 WP_418140337.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  NUW46_RS13415 (NUW46_13440) rfbA 2900074..2900946 (-) 873 WP_418140338.1 glucose-1-phosphate thymidylyltransferase RfbA -
  NUW46_RS13420 (NUW46_13445) rfbB 2900943..2902016 (-) 1074 WP_418140339.1 dTDP-glucose 4,6-dehydratase -
  NUW46_RS13425 (NUW46_13450) - 2902013..2902981 (-) 969 WP_418140340.1 Wzz/FepE/Etk N-terminal domain-containing protein -
  NUW46_RS13430 (NUW46_13455) - 2902981..2905680 (-) 2700 WP_418140341.1 SLBB domain-containing protein -
  NUW46_RS13435 (NUW46_13460) - 2905714..2907195 (-) 1482 WP_418140342.1 capsule assembly Wzi family protein -
  NUW46_RS13440 (NUW46_13465) - 2907416..2908174 (-) 759 WP_418140343.1 Wzz/FepE/Etk N-terminal domain-containing protein -
  NUW46_RS13445 (NUW46_13470) rfaH 2908269..2908748 (-) 480 WP_418140344.1 transcription/translation regulatory transformer protein RfaH -
  NUW46_RS13450 (NUW46_13475) cysQ 2908745..2909521 (-) 777 WP_418140345.1 3'(2'),5'-bisphosphate nucleotidase CysQ -
  NUW46_RS13455 (NUW46_13480) - 2909612..2910973 (+) 1362 WP_418140346.1 MBL fold metallo-hydrolase -
  NUW46_RS13460 (NUW46_13485) - 2910992..2911435 (-) 444 WP_418140347.1 pilin -
  NUW46_RS13465 (NUW46_13495) - 2911518..2913338 (-) 1821 WP_418140348.1 Wzy polymerase domain-containing protein -
  NUW46_RS13470 (NUW46_13500) - 2913335..2914765 (-) 1431 WP_418140349.1 O-antigen ligase family protein -
  NUW46_RS13475 (NUW46_13505) - 2914833..2915306 (-) 474 WP_418140350.1 pilin -
  NUW46_RS13480 (NUW46_13510) pilB 2915738..2917453 (+) 1716 WP_418140351.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62872.01 Da        Isoelectric Point: 4.9077

>NTDB_id=721257 NUW46_RS13480 WP_418140351.1 2915738..2917453(+) (pilB) [Marinobacter sp. MA]
MSTNKSNITLTGLARRFVDDGLLDEATAKDAFLQASQNRIPLITYLTQNNLADSSKLAFSAAMEFGVSVLDLSAFMPEMM
PEKVVDEKLVRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAVLVDDAKLREAIDKYLESQDTTMGDLDDS
DLEGVETEGGEQEDDGAVVASEVDDAPIVKYVNKMLLDAIRGGASGIHFEPYEKVYRVRYRTDGILKEVSRPSIKLAPKI
SARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEDQKQMYMDALK
QPQGMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPAEINLEGINQVNVNNRVGLGFAEALRAFLRQDPDVIMVGE
IRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPSFNIATSVSLIIAQRLGRRLCSACKQPFEVPKDVLLEE
GFTQEQIDTGFTLYRPKGCDKCNGGYKGRVGIYEVVKITDELANMIMEEASSIKIAKQAQAEGFRNLRQSALMKVIEGVT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=721257 NUW46_RS13480 WP_418140351.1 2915738..2917453(+) (pilB) [Marinobacter sp. MA]
ATGTCTACCAATAAGAGCAATATCACGCTTACGGGCCTTGCGAGGCGCTTCGTGGATGACGGACTGCTGGATGAAGCAAC
GGCGAAAGATGCTTTTCTTCAGGCCTCACAGAACCGCATTCCGCTAATCACCTACCTGACTCAGAACAACCTTGCAGATA
GCTCCAAACTTGCGTTTTCGGCAGCCATGGAATTCGGAGTTTCTGTGCTAGATCTTTCCGCATTCATGCCAGAAATGATG
CCGGAAAAGGTGGTTGACGAAAAACTTGTCCGTAAACACAACGCCCTTCCTCTTTATAAAAGAGGAAATCGGCTGTTCAT
TGCCGTTTCAGATCCGACCAACATCCAAGCCCTGGACGAGATCAAATTCAACACTGGCCTGAGCACTGATGCGGTGTTGG
TGGACGACGCCAAGCTTCGGGAAGCTATCGACAAGTACCTGGAATCCCAAGATACCACCATGGGTGATCTGGATGACTCA
GACCTTGAAGGTGTTGAAACCGAGGGTGGTGAACAGGAAGACGATGGCGCCGTTGTTGCCAGTGAGGTGGATGACGCACC
CATAGTTAAGTATGTTAACAAGATGCTGCTAGATGCGATTCGCGGCGGCGCCTCGGGTATTCACTTCGAGCCCTATGAAA
AAGTCTATCGGGTAAGATACCGCACCGACGGTATTTTGAAAGAAGTCTCTCGCCCCTCTATTAAGCTGGCTCCTAAGATT
TCCGCCCGCGTGAAAATCATGGCCCAGTTGGATATTTCCGAGAGACGCGTACCCCAAGACGGCCGAATCAAGATGAAGCT
GTCCAAGACCAAAGCCATCGATTTTCGGGTGAACACTCTGCCCACTCTCTGGGGTGAGAAGATCGTTTTGCGGATTCTTG
ATCCCAGCCAGGCGAAACTGGGCATTGATGTGCTCGGTTATGAGGAGGACCAGAAACAGATGTACATGGATGCCTTGAAA
CAGCCACAGGGCATGATTCTCGTGACTGGCCCCACCGGCTCCGGCAAAACCGTTTCACTGTATACCGGACTGAATATTCT
GAACACTCCAGACATCAACATCTCCACTGCGGAAGACCCGGCAGAGATCAACCTGGAGGGCATCAACCAGGTAAACGTAA
ACAACCGCGTGGGTCTGGGTTTTGCCGAGGCTCTGCGCGCGTTTCTGCGTCAGGATCCCGATGTGATCATGGTAGGTGAG
ATCCGGGATCTTGAAACCGCCAACATTGCTATCAAAGCGGCACAGACCGGGCATCTGGTTCTTTCCACCCTCCACACGAA
TAGTGCTGCAGAGACTCTCACGCGAATGATGAACATGGGCGTTCCCTCTTTCAACATCGCTACTTCGGTGAGCCTGATTA
TTGCCCAGCGTCTCGGCCGGAGACTCTGCAGCGCATGTAAACAGCCCTTCGAAGTGCCGAAAGACGTGCTTTTGGAGGAG
GGGTTCACACAGGAACAAATTGATACAGGCTTCACGTTGTACCGCCCCAAGGGCTGTGATAAATGCAATGGAGGATATAA
AGGCCGCGTCGGGATTTACGAGGTGGTCAAGATTACCGACGAGCTGGCCAACATGATTATGGAAGAAGCCAGTTCCATCA
AAATTGCAAAGCAGGCTCAGGCCGAGGGCTTCCGTAACTTGCGCCAATCGGCACTTATGAAAGTGATCGAGGGCGTAACA
AGTCTTGAGGAAGCCAACCGCGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.944

100

0.574
  pilB Acinetobacter baylyi ADP1

56.294

100

0.564
  pilB Legionella pneumophila strain ERS1305867

53.71

99.124

0.532
  pilB Vibrio parahaemolyticus RIMD 2210633

51.526

97.548

0.503
  pilB Vibrio campbellii strain DS40M4

50.532

98.774

0.499
  pilB Vibrio cholerae strain A1552

54.143

90.893

0.492
  pilF Neisseria gonorrhoeae MS11

47.611

98.949

0.471
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.114

92.119

0.37
  pilB/pilB1 Synechocystis sp. PCC 6803

40.27

90.893

0.366