Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NUW46_RS03850 Genome accession   NZ_CP103058
Coordinates   839022..840083 (+) Length   353 a.a.
NCBI ID   WP_008170799.1    Uniprot ID   G6YPX9
Organism   Marinobacter sp. MA     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 834022..845083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUW46_RS03835 (NUW46_03845) rpmE 834514..834735 (+) 222 WP_064230272.1 50S ribosomal protein L31 -
  NUW46_RS03840 (NUW46_03850) - 834965..836230 (+) 1266 WP_418141402.1 malic enzyme-like NAD(P)-binding protein -
  NUW46_RS03845 (NUW46_03855) - 836298..838826 (-) 2529 WP_418141403.1 penicillin-binding protein 1A -
  NUW46_RS03850 (NUW46_03860) pilM 839022..840083 (+) 1062 WP_008170799.1 pilus assembly protein PilM Machinery gene
  NUW46_RS03855 (NUW46_03865) - 840083..840646 (+) 564 WP_088829301.1 PilN domain-containing protein -
  NUW46_RS03860 (NUW46_03870) - 840646..841257 (+) 612 WP_222466552.1 type 4a pilus biogenesis protein PilO -
  NUW46_RS03865 (NUW46_03875) - 841260..841808 (+) 549 WP_222466553.1 pilus assembly protein PilP -
  NUW46_RS03870 (NUW46_03880) pilQ 841874..843940 (+) 2067 WP_418141404.1 type IV pilus secretin PilQ Machinery gene
  NUW46_RS03875 (NUW46_03885) aroK 844077..844643 (+) 567 WP_418141405.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 37794.84 Da        Isoelectric Point: 4.1452

>NTDB_id=721244 NUW46_RS03850 WP_008170799.1 839022..840083(+) (pilM) [Marinobacter sp. MA]
MFGLGKKSSAVLGVDISSSSVKLLELSKQGDRYKVESYAVEPLPANAVVEKNITDVEAVGEVMKRVASKSRSGVKQVAVA
VSGSAVITKVIQMDGGLNEFEMEDQIALEADQYIPYPLDEVAIDFEVQGPSESNPDQVDVLLAACRKENVDIREDALEIA
SLTTKVVDVEAYALERAYALIEPQLDSQGEELVVAIVDIGATMTTLSVLAGGKTVYTREQIFGGKQLTEEIQRRYGLSLE
EAGLAKKQGGLPDDYESEVLTPFREAVVQQVARALQFFFGASQYNAVDYVVLAGGTASIQGLTEMVEEKTGTPTMVANPF
ADMAVGSRVNASALSNDAPSLMIACGLAMRSFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=721244 NUW46_RS03850 WP_008170799.1 839022..840083(+) (pilM) [Marinobacter sp. MA]
GTGTTTGGATTAGGAAAGAAATCCAGTGCAGTGCTGGGCGTGGATATAAGCTCCAGCTCGGTCAAACTTCTGGAGCTGTC
GAAGCAGGGCGACCGGTATAAGGTCGAGAGCTACGCGGTTGAGCCATTACCGGCCAATGCCGTGGTGGAAAAGAACATTA
CCGACGTGGAAGCCGTGGGCGAAGTGATGAAGCGCGTGGCTTCCAAGTCCCGTTCCGGCGTAAAGCAGGTAGCAGTTGCG
GTATCAGGTTCGGCGGTAATCACCAAGGTCATCCAGATGGATGGTGGCCTCAACGAATTCGAGATGGAAGATCAGATTGC
CCTCGAGGCCGATCAGTATATTCCATACCCGCTGGATGAAGTGGCCATCGATTTTGAGGTTCAGGGACCTTCCGAAAGTA
ACCCCGACCAGGTAGATGTGCTTCTGGCAGCATGCCGAAAGGAAAATGTCGACATTCGGGAAGATGCACTCGAGATTGCT
TCACTCACCACCAAGGTGGTTGATGTTGAGGCCTATGCCCTTGAGCGCGCCTACGCTTTGATCGAGCCGCAGCTGGACTC
TCAGGGCGAAGAATTGGTGGTGGCGATTGTTGATATCGGCGCCACGATGACCACGCTGAGCGTTTTGGCCGGCGGCAAAA
CCGTTTACACCCGCGAACAGATTTTTGGTGGCAAGCAACTGACCGAGGAAATTCAGCGTCGCTATGGTCTGTCTCTGGAG
GAAGCTGGCCTTGCCAAGAAACAGGGCGGACTGCCCGATGACTACGAATCCGAAGTTCTGACCCCGTTTCGCGAAGCCGT
GGTGCAGCAAGTGGCCCGAGCGCTTCAGTTCTTCTTTGGTGCCAGTCAGTACAATGCTGTCGACTATGTGGTTTTGGCAG
GTGGTACTGCCTCGATTCAGGGCCTGACGGAAATGGTCGAAGAAAAAACGGGTACGCCGACTATGGTCGCGAATCCTTTT
GCAGACATGGCGGTTGGGTCTCGGGTCAACGCATCGGCACTGAGTAACGATGCGCCGTCTCTGATGATTGCCTGCGGCCT
GGCAATGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G6YPX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

54.023

98.584

0.533
  comM Acinetobacter nosocomialis M2

54.023

98.584

0.533
  comM Acinetobacter baylyi ADP1

51.724

98.584

0.51
  pilM Legionella pneumophila strain ERS1305867

46.571

99.15

0.462