Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   PUW74_RS00410 Genome accession   NZ_CP118080
Coordinates   64841..65839 (+) Length   332 a.a.
NCBI ID   WP_000196633.1    Uniprot ID   Q3K3X8
Organism   Streptococcus agalactiae strain VSI14     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 59841..70839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW74_RS00395 (PUW74_00395) - 60868..62259 (+) 1392 WP_001079797.1 hypothetical protein -
  PUW74_RS00400 (PUW74_00400) purB 62281..63579 (+) 1299 WP_000572890.1 adenylosuccinate lyase -
  PUW74_RS00405 (PUW74_00405) comR 63732..64640 (+) 909 WP_000912095.1 XRE family transcriptional regulator Regulator
  PUW74_RS00410 (PUW74_00410) ruvB 64841..65839 (+) 999 WP_000196633.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  PUW74_RS00415 (PUW74_00415) - 65991..66428 (+) 438 WP_000754818.1 low molecular weight protein-tyrosine-phosphatase -
  PUW74_RS00420 (PUW74_00420) - 66435..66815 (+) 381 WP_000787702.1 membrane protein -
  PUW74_RS00425 (PUW74_00425) - 66812..68590 (+) 1779 WP_001220907.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 37573.90 Da        Isoelectric Point: 4.5053

>NTDB_id=721168 PUW74_RS00410 WP_000196633.1 64841..65839(+) (ruvB) [Streptococcus agalactiae strain VSI14]
MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEAAKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAIEKSGDLVAILNDLEPGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPRIANRLLKRVRDYAQIMGDGLID
DNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGGPVGLGTLSVNIAEERDTVEDMYEPYLIQKGFIMRTRTGRVATDK
AYEHLGYQRFDK

Nucleotide


Download         Length: 999 bp        

>NTDB_id=721168 PUW74_RS00410 WP_000196633.1 64841..65839(+) (ruvB) [Streptococcus agalactiae strain VSI14]
ATGACAAGATTTTTAGATAGTGATGCAATGGGTGACGAAGAATTGGTAGAACGTACACTTCGTCCGCAGTATTTAAGAGA
GTATATTGGACAAGATAAGGTTAAAGATCAGCTAAAAATATTTATTGAAGCTGCTAAATTGCGTGATGAGTCATTGGATC
ATGTGTTATTATTTGGCCCTCCTGGTTTAGGGAAAACAACCATGGCATTTGTAATTGCTAATGAGTTGGGTGTCAATCTC
AAACAAACATCAGGTCCCGCAATTGAAAAATCAGGGGATTTAGTAGCCATTTTAAATGATTTAGAACCAGGTGATGTTCT
TTTTATTGATGAAATTCATCGTATGCCGATGGCGGTTGAAGAGGTACTTTATAGTGCAATGGAAGACTTTTATATTGACA
TTATGATCGGTGCAGGAGAAACTAGTAGAAGTGTTCATTTAGATTTGCCGCCCTTTACCTTAATTGGTGCAACGACACGT
GCAGGTATGTTATCTAATCCCTTACGTGCTCGCTTTGGTATTACAGGGCATATGGAGTATTATGAAGAAAACGATTTGAC
AGAAATTATTGAGCGTACAGCAGATATTTTTGAAATGAAAATTACTTATGAAGCTGCTTCTGAATTAGCGCGTCGCAGTC
GTGGAACGCCACGTATCGCTAACCGTTTATTGAAACGTGTTCGAGATTATGCTCAAATCATGGGAGATGGTTTGATAGAT
GACAATATTACAGATAAAGCATTAACGATGTTAGATGTTGATCACGAGGGGCTTGATTACGTCGATCAAAAAATCTTAAG
AACCATGATTGAAATGTATAATGGAGGTCCTGTTGGTTTAGGAACTCTATCCGTTAATATTGCTGAAGAACGAGATACTG
TTGAAGACATGTACGAACCTTATTTAATTCAAAAAGGTTTTATTATGCGTACCCGTACCGGTCGTGTAGCTACGGATAAG
GCATACGAACATTTAGGTTATCAGCGATTTGATAAATAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(20-178)

RuvB C-terminal winged helix domain (WHD_RuvB)

(256-325)

RuvB AAA lid domain (AAA_lid_4)

(181-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3K3X8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Streptococcus pneumoniae TIGR4

90.06

100

0.901
  ruvB Streptococcus pneumoniae R6

89.759

100

0.898
  ruvB Streptococcus pneumoniae D39

89.759

100

0.898
  ruvB Bacillus subtilis subsp. subtilis str. 168

59.819

99.699

0.596
  ruvB Helicobacter pylori 26695

53.074

93.072

0.494
  ruvB Synechocystis sp. PCC 6803

50.479

94.277

0.476