Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NW971_RS08125 Genome accession   NZ_CP102962
Coordinates   1653478..1654452 (-) Length   324 a.a.
NCBI ID   WP_258464275.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 16CS0212     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1648478..1659452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NW971_RS08085 (NW971_08085) gcvT 1648979..1650070 (-) 1092 WP_258464271.1 glycine cleavage system aminomethyltransferase GcvT -
  NW971_RS08090 (NW971_08090) - 1650229..1650753 (-) 525 WP_258464272.1 shikimate kinase -
  NW971_RS08095 (NW971_08095) - 1650743..1650889 (-) 147 WP_001789879.1 hypothetical protein -
  NW971_RS08100 (NW971_08100) comGF 1650986..1651483 (-) 498 WP_029693246.1 competence type IV pilus minor pilin ComGF Machinery gene
  NW971_RS08105 (NW971_08105) comGE 1651401..1651700 (-) 300 WP_258464273.1 competence protein ComGE Machinery gene
  NW971_RS08110 (NW971_08110) comGD 1651687..1652133 (-) 447 WP_258464274.1 competence type IV pilus minor pilin ComGD Machinery gene
  NW971_RS08115 (NW971_08115) comGC 1652111..1652422 (-) 312 WP_111762334.1 competence type IV pilus major pilin ComGC Machinery gene
  NW971_RS08120 (NW971_08120) comGB 1652436..1653506 (-) 1071 WP_000776405.1 competence type IV pilus assembly protein ComGB Machinery gene
  NW971_RS08125 (NW971_08125) comGA 1653478..1654452 (-) 975 WP_258464275.1 competence type IV pilus ATPase ComGA Machinery gene
  NW971_RS08130 (NW971_08130) - 1654504..1655127 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  NW971_RS08135 (NW971_08135) - 1655124..1655453 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  NW971_RS08140 (NW971_08140) - 1655453..1656439 (-) 987 WP_000161315.1 ROK family glucokinase -
  NW971_RS08145 (NW971_08145) - 1656436..1656639 (-) 204 WP_258464276.1 YqgQ family protein -
  NW971_RS08150 (NW971_08150) - 1656620..1658083 (-) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  NW971_RS08155 (NW971_08155) - 1658095..1658634 (-) 540 WP_258464277.1 5-formyltetrahydrofolate cyclo-ligase -
  NW971_RS08160 (NW971_08160) rpmG 1658843..1658992 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36923.99 Da        Isoelectric Point: 8.8405

>NTDB_id=720627 NW971_RS08125 WP_258464275.1 1653478..1654452(-) (comGA) [Staphylococcus aureus strain 16CS0212]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
YKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNNSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=720627 NW971_RS08125 WP_258464275.1 1653478..1654452(-) (comGA) [Staphylococcus aureus strain 16CS0212]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
TATAAAATATACTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGGGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATAATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.383

100

0.994
  comGA Staphylococcus aureus N315

99.383

100

0.994