Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   PUN50_RS11625 Genome accession   NZ_CP117988
Coordinates   2522316..2523320 (-) Length   334 a.a.
NCBI ID   WP_005426235.1    Uniprot ID   A7N1I0
Organism   Vibrio campbellii strain 20220413_1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2517316..2528320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN50_RS11595 (PUN50_11595) tolQ 2517420..2518118 (-) 699 WP_005426224.1 protein TolQ -
  PUN50_RS11600 (PUN50_11600) ybgC 2518108..2518527 (-) 420 WP_274290430.1 tol-pal system-associated acyl-CoA thioesterase -
  PUN50_RS11605 (PUN50_11605) ybgE 2518699..2519001 (-) 303 WP_005426209.1 cyd operon protein YbgE -
  PUN50_RS11610 (PUN50_11610) cydX 2518994..2519101 (-) 108 WP_000270284.1 cytochrome bd-I oxidase subunit CydX -
  PUN50_RS11615 (PUN50_11615) cydB 2519114..2520250 (-) 1137 WP_038889166.1 cytochrome d ubiquinol oxidase subunit II -
  PUN50_RS11620 (PUN50_11620) cydA 2520268..2521854 (-) 1587 WP_038889167.1 cytochrome ubiquinol oxidase subunit I -
  PUN50_RS11625 (PUN50_11625) ruvB 2522316..2523320 (-) 1005 WP_005426235.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  PUN50_RS11630 (PUN50_11630) ruvA 2523342..2523956 (-) 615 WP_005532773.1 Holliday junction branch migration protein RuvA Machinery gene
  PUN50_RS11635 (PUN50_11635) - 2524035..2525486 (-) 1452 WP_274290431.1 methyl-accepting chemotaxis protein -
  PUN50_RS11640 (PUN50_11640) ruvC 2525609..2526130 (-) 522 WP_005426231.1 crossover junction endodeoxyribonuclease RuvC -
  PUN50_RS11645 (PUN50_11645) aspS 2526231..2528009 (-) 1779 WP_038889171.1 aspartate--tRNA ligase -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37277.63 Da        Isoelectric Point: 4.6703

>NTDB_id=720204 PUN50_RS11625 WP_005426235.1 2522316..2523320(-) (ruvB) [Vibrio campbellii strain 20220413_1]
MIEADRLIAPENPSFREEDVIDRAIRPKKLADYQGQDHVRDQMEIFIKAAQLRNEALDHLLIFGPPGLGKTTLANIVANE
MEVNIRTTSGPVLEKAGDLAALLTNLEENDVLFIDEIHRLSPMVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLI
GATTRAGSLTSPLRDRFGITQRLEYYKVKDLQDIVQRSADCLGLSMESEGALEVARRARGTPRIANRLLRRVRDYAEVKG
NGHICADTADKALNMLDVDAEGFDYMDRKLLLAIMEKFGGGPVGLDNMAAAIGEEKDTIEDVLEPYLIQQGYLQRTPRGR
IATDRAYLHFGIEK

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=720204 PUN50_RS11625 WP_005426235.1 2522316..2523320(-) (ruvB) [Vibrio campbellii strain 20220413_1]
ATGATTGAAGCCGATCGTTTAATCGCGCCAGAAAATCCTTCGTTTCGTGAAGAAGACGTCATCGACCGTGCGATTCGTCC
TAAGAAGCTGGCGGATTACCAAGGTCAGGATCACGTGCGTGATCAGATGGAGATTTTCATCAAAGCCGCTCAATTACGAA
ATGAAGCGCTCGACCACCTTTTGATTTTTGGTCCTCCAGGTTTGGGTAAAACCACGCTTGCGAACATCGTTGCCAATGAG
ATGGAAGTGAACATTCGTACCACTTCAGGCCCTGTACTTGAGAAAGCAGGGGACTTAGCGGCGCTGTTGACCAACTTAGA
AGAAAACGATGTGTTGTTCATTGATGAGATCCACCGCTTGAGCCCGATGGTTGAAGAGGTGTTGTATCCAGCGATGGAAG
ACTACCAGTTAGACATTATGATTGGTGAAGGTCCGGCGGCGCGCTCTATTAAAATTGATTTACCACCTTTCACTTTGATT
GGCGCAACGACGCGTGCAGGCTCTCTGACATCACCATTGCGTGACCGTTTTGGTATTACTCAGCGTCTTGAATACTACAA
AGTGAAAGACCTGCAAGATATCGTTCAGCGTAGTGCTGACTGTTTGGGTTTGTCTATGGAGTCGGAAGGGGCGTTAGAAG
TTGCTCGCCGTGCTCGTGGCACGCCACGTATTGCTAACCGATTGCTACGCCGAGTTCGTGACTATGCGGAAGTAAAAGGC
AACGGTCATATTTGTGCTGATACCGCAGACAAAGCATTGAACATGCTTGATGTCGATGCCGAAGGTTTCGATTATATGGA
CCGTAAGCTGCTGTTAGCGATCATGGAGAAGTTTGGTGGTGGACCTGTTGGTTTGGACAACATGGCAGCGGCGATCGGTG
AAGAGAAAGACACCATCGAAGACGTTCTTGAACCATATCTAATCCAACAAGGCTATTTGCAACGCACTCCGCGTGGTAGA
ATTGCCACCGATCGTGCTTATTTGCATTTCGGAATTGAAAAGTAA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(261-330)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(25-183)

RuvB AAA lid domain (AAA_lid_4)

(186-259)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7N1I0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

98.503

0.596
  ruvB Streptococcus pneumoniae TIGR4

58.824

96.707

0.569
  ruvB Streptococcus pneumoniae R6

58.824

96.707

0.569
  ruvB Streptococcus pneumoniae D39

58.824

96.707

0.569
  ruvB Synechocystis sp. PCC 6803

53.681

97.605

0.524
  ruvB Helicobacter pylori 26695

54.276

91.018

0.494