Detailed information NTG0719975

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: MKL45_RS16495
• Genome accession: NZ_CP102934
• Coordinates: 3473822..3474880 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter sp. AOR07_HL
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3468822..3479880

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MKL45_RS16470 (MKL45_16475)	aroK	3469097..3469666 (-)	570	WP_000643712.1	shikimate kinase AroK	-
MKL45_RS16475 (MKL45_16480)	pilQ	3469678..3471843 (-)	2166	WP_002062175.1	type IV pilus secretin PilQ family protein	Machinery gene
MKL45_RS16480 (MKL45_16485)	pilP	3471906..3472433 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
MKL45_RS16485 (MKL45_16490)	pilO	3472444..3473184 (-)	741	WP_000081156.1	type 4a pilus biogenesis protein PilO	Machinery gene
MKL45_RS16490 (MKL45_16495)	pilN	3473181..3473822 (-)	642	WP_000201224.1	PilN domain-containing protein	Machinery gene
MKL45_RS16495 (MKL45_16500)	pilM	3473822..3474880 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
MKL45_RS16500 (MKL45_16505)	ponA	3475044..3477599 (+)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
MKL45_RS16505 (MKL45_16510)	-	3477617..3478441 (-)	825	WP_002125608.1	methyltransferase domain-containing protein	-
MKL45_RS16510 (MKL45_16515)	-	3478444..3478776 (-)	333	WP_000169599.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=719975 MKL45_RS16495 WP_002014066.1 3473822..3474880(-) (pilM) [Acinetobacter sp. AOR07_HL]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=719975 MKL45_RS16495 WP_002014066.1 3473822..3474880(-) (pilM) [Acinetobacter sp. AOR07_HL]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCCGAGCAAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCTAAGCTTTTGCAGCAAAAATTGGGCTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGATGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423