Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NVV93_RS02335 Genome accession   NZ_CP102830
Coordinates   508042..509106 (+) Length   354 a.a.
NCBI ID   WP_258252854.1    Uniprot ID   -
Organism   Pseudomonas sp. LS44     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 503042..514106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NVV93_RS02325 (NVV93_02325) - 504089..505357 (+) 1269 WP_258252852.1 malic enzyme-like NAD(P)-binding protein -
  NVV93_RS02330 (NVV93_02330) - 505427..507856 (-) 2430 WP_258252853.1 penicillin-binding protein 1A -
  NVV93_RS02335 (NVV93_02335) pilM 508042..509106 (+) 1065 WP_258252854.1 pilus assembly protein PilM Machinery gene
  NVV93_RS02340 (NVV93_02340) pilN 509106..509678 (+) 573 WP_258252855.1 type 4a pilus biogenesis protein PilN -
  NVV93_RS02345 (NVV93_02345) pilO 509675..510298 (+) 624 WP_258252856.1 type 4a pilus biogenesis protein PilO -
  NVV93_RS02350 (NVV93_02350) pilP 510328..510822 (+) 495 WP_258254269.1 type 4a pilus biogenesis lipoprotein PilP -
  NVV93_RS02355 (NVV93_02355) pilQ 510877..512943 (+) 2067 WP_258252857.1 type IV pilus secretin PilQ Machinery gene
  NVV93_RS02360 (NVV93_02360) aroK 512948..513466 (+) 519 WP_258252858.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37998.42 Da        Isoelectric Point: 4.5399

>NTDB_id=719135 NVV93_RS02335 WP_258252854.1 508042..509106(+) (pilM) [Pseudomonas sp. LS44]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALARVLAKAKTGVKTVAV
AVAGSAVITKTIEMEAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQRASARNADRVDVLLAACRKENVEVREAALAL
SGMTAKVVDVEAYALERAYGLLAPQFGNGHDEMTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTDEIQRRYGLSV
EEAGLAKKQGGLPDDYEGEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSSRVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=719135 NVV93_RS02335 WP_258252854.1 508042..509106(+) (pilM) [Pseudomonas sp. LS44]
GTGCTAGGGCTCTTCAATAAGAAAGCGAATACGCTTCTGGGGATAGATATCAGTTCGACCTCAGTCAAGCTCTTGGAATT
GAGTCGCTCGGGAAGCCGCTACAAAGTAGAGGCTTACGCCGTCGAGCCCCTCCCACCTAACGCCGTGGTTGAGAAGAACA
TTGTCGAGCTGGAGGGGGTCGGTCAGGCGCTGGCCCGTGTTCTCGCCAAAGCCAAAACTGGCGTAAAGACGGTCGCAGTG
GCTGTTGCGGGCTCGGCTGTCATTACAAAGACCATCGAGATGGAAGCCGGTCTCTCCGACGATGAGCTGGAAAACCAGCT
GAAGATCGAAGCCGATCAATACATTCCTTACCCGCTGGAAGAAGTCGCGATCGATTTCGAAGTGCAGAGAGCTTCAGCGC
GGAACGCTGATCGAGTGGACGTATTGCTCGCGGCATGCCGAAAGGAAAATGTCGAGGTGCGCGAAGCTGCCTTGGCGCTG
TCGGGGATGACTGCGAAGGTAGTTGACGTCGAGGCGTACGCCTTGGAGCGTGCCTACGGGTTACTCGCCCCGCAGTTTGG
CAATGGTCATGACGAAATGACCGTCGCGGTAGTCGATATCGGCGCAACCATGACCACGCTGAGCGTGCTGCACAATGGGC
GCACTATATATACCCGCGAACAGTTGTTTGGCGGCAAGCAGTTGACCGATGAAATCCAGCGCCGTTACGGCTTATCGGTT
GAAGAGGCGGGGCTTGCCAAAAAACAGGGTGGTTTACCGGATGATTATGAAGGTGAAGTGCTGCAGCCGTTTAAAGAGGC
CGTAGTGCAACAGGTTTCGCGATCGCTGCAGTTTTTCTTCGCAGCCGGTCAATTCAACGATGTCGACTACATCCTCTTGG
CTGGTGGTACGGCTTCTATTCCTGATTTGGATCGCCTGATTCAGCAAAAAATTGGGACTCAGACTTTAGTGGCTAATCCA
TTTGCCGATATGGCCTTAAGCAGCAGAGTTAACGCTGGCGCTCTGGCTAGCGACGCTCCGGCACTAATGATTGCGTGTGG
GCTGGCCATGAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

52.825

100

0.528
  pilM Legionella pneumophila strain ERS1305867

45.198

100

0.452