Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   KQ693_RS00215 Genome accession   NZ_CP102606
Coordinates   42141..42764 (+) Length   207 a.a.
NCBI ID   WP_267261564.1    Uniprot ID   -
Organism   Thermus sp. PS18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 37141..47764
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ693_RS00190 (KQ693_00190) - 37173..38588 (+) 1416 WP_267261561.1 HD-GYP domain-containing protein -
  KQ693_RS00195 (KQ693_00195) - 38624..39133 (+) 510 WP_267262389.1 DUF5317 domain-containing protein -
  KQ693_RS00200 (KQ693_00200) - 39206..40210 (+) 1005 WP_267261562.1 homoisocitrate dehydrogenase -
  KQ693_RS00205 (KQ693_00205) pilM 40402..41535 (+) 1134 WP_219760144.1 type IV pilus assembly protein PilM Machinery gene
  KQ693_RS00210 (KQ693_00210) pilN 41528..42151 (+) 624 WP_267261563.1 flagellar protein FliT Machinery gene
  KQ693_RS00215 (KQ693_00215) pilO 42141..42764 (+) 624 WP_267261564.1 type 4a pilus biogenesis protein PilO Machinery gene
  KQ693_RS00220 (KQ693_00220) pilW 42761..43732 (+) 972 WP_267261565.1 competence protein Machinery gene
  KQ693_RS00225 (KQ693_00225) pilQ 43713..45890 (+) 2178 WP_267261566.1 secretin N-terminal domain-containing protein Machinery gene
  KQ693_RS00230 (KQ693_00230) aroC 45994..47145 (+) 1152 WP_267261567.1 chorismate synthase -
  KQ693_RS00235 (KQ693_00235) - 47163..47717 (+) 555 WP_028493414.1 shikimate kinase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22905.42 Da        Isoelectric Point: 9.8887

>NTDB_id=718103 KQ693_RS00215 WP_267261564.1 42141..42764(+) (pilO) [Thermus sp. PS18]
MLARLGQREWALIAIALTLVVALLWYFFLIVPMRQETESVRQEINALIPERDKGRQAQRALPELRATIAELQAERQAFLR
ALPKEERLSQVLNEILTEALRSGVTVRSFTRSPTSAPVPEVRAVNLALSLEAPFPETYTYLKRLEGLSRFSSLSGLNLSV
QGQDLNPLLSTSLTLTLYMLAKDLAQPQANPQTQGAPSPQTGQGGGR

Nucleotide


Download         Length: 624 bp        

>NTDB_id=718103 KQ693_RS00215 WP_267261564.1 42141..42764(+) (pilO) [Thermus sp. PS18]
GTGCTCGCTAGATTGGGACAGCGGGAGTGGGCCCTGATCGCCATCGCCCTCACCTTGGTGGTGGCCCTCCTCTGGTACTT
TTTCCTCATCGTCCCCATGCGTCAGGAAACGGAAAGCGTGCGCCAGGAGATCAACGCCCTTATCCCCGAGCGGGATAAAG
GAAGGCAAGCCCAGCGCGCCCTTCCAGAACTCCGGGCCACCATCGCTGAGCTGCAAGCCGAGCGCCAAGCCTTTCTGAGG
GCACTTCCCAAGGAGGAACGGCTTTCCCAGGTTCTGAACGAGATCCTGACCGAGGCCCTAAGGAGCGGGGTCACGGTGCG
CTCCTTCACCCGCTCCCCCACCTCGGCTCCAGTACCGGAGGTGCGGGCGGTGAACCTGGCCCTTTCCCTCGAGGCGCCCT
TCCCCGAAACCTATACCTACTTGAAGCGCCTGGAAGGACTTTCCCGTTTCAGCTCCCTTTCCGGGCTCAACCTCAGCGTC
CAGGGCCAGGACTTGAATCCCCTTCTCTCCACCAGCTTGACCCTTACCCTCTACATGCTGGCCAAGGACCTTGCCCAGCC
CCAAGCAAACCCCCAGACGCAAGGAGCTCCATCCCCGCAAACAGGTCAAGGAGGTGGTCGGTGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(46-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Thermus thermophilus HB27

72.826

88.889

0.647