Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   KQ693_RS00205 Genome accession   NZ_CP102606
Coordinates   40402..41535 (+) Length   377 a.a.
NCBI ID   WP_219760144.1    Uniprot ID   -
Organism   Thermus sp. PS18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 35402..46535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ693_RS00175 (KQ693_00175) - 35909..36274 (-) 366 WP_267261560.1 hypothetical protein -
  KQ693_RS00190 (KQ693_00190) - 37173..38588 (+) 1416 WP_267261561.1 HD-GYP domain-containing protein -
  KQ693_RS00195 (KQ693_00195) - 38624..39133 (+) 510 WP_267262389.1 DUF5317 domain-containing protein -
  KQ693_RS00200 (KQ693_00200) - 39206..40210 (+) 1005 WP_267261562.1 homoisocitrate dehydrogenase -
  KQ693_RS00205 (KQ693_00205) pilM 40402..41535 (+) 1134 WP_219760144.1 type IV pilus assembly protein PilM Machinery gene
  KQ693_RS00210 (KQ693_00210) pilN 41528..42151 (+) 624 WP_267261563.1 flagellar protein FliT Machinery gene
  KQ693_RS00215 (KQ693_00215) pilO 42141..42764 (+) 624 WP_267261564.1 type 4a pilus biogenesis protein PilO Machinery gene
  KQ693_RS00220 (KQ693_00220) pilW 42761..43732 (+) 972 WP_267261565.1 competence protein Machinery gene
  KQ693_RS00225 (KQ693_00225) pilQ 43713..45890 (+) 2178 WP_267261566.1 secretin N-terminal domain-containing protein Machinery gene

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41334.91 Da        Isoelectric Point: 4.4207

>NTDB_id=718101 KQ693_RS00205 WP_219760144.1 40402..41535(+) (pilM) [Thermus sp. PS18]
MLSGFSKLFKPRVEALGLEIGAANLKLVELSGNPPALRTLATRPTPPGMLVEGVIAEPQALAQELKELLAEARTKKRYVV
TAVPNPSVILRTLQVPKMPLKEMEEAVRWEAERYIPFPIDEVVLDFAPLDPLAEVAEGEQVEVMVGAARQEAVASLLEAL
RGAGLTPIILDVKPFAGLYPLEAQLSSDPEGVSVAVEIGAESTSLVLLKGNRPLAVRILTLSGKDFTEAIGKSFGLDFLT
AEEVKRTYGLATIPTEDEELLLDFDAERERYSPAKIYDAIRPVLVELTQEIRRSLEFFRVQLGDIQPEVGYLYGGGSRLR
GLSTLLTDTLGVNFTVPDPWQGIQVDPRRFDLEKIKEMEPEFLVPVGLALRGVMPLD

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=718101 KQ693_RS00205 WP_219760144.1 40402..41535(+) (pilM) [Thermus sp. PS18]
GTGCTTTCGGGTTTTAGCAAGCTATTCAAACCCCGCGTGGAGGCGCTGGGCCTGGAGATCGGCGCCGCCAACCTCAAACT
GGTGGAACTCTCCGGAAATCCTCCCGCCCTCAGGACCCTGGCCACCCGGCCCACTCCCCCGGGAATGCTGGTGGAAGGGG
TGATCGCCGAGCCCCAGGCCCTGGCTCAGGAACTCAAGGAACTTCTCGCCGAAGCCCGGACCAAAAAGCGCTACGTGGTG
ACCGCCGTGCCCAATCCCAGCGTCATCCTGCGCACCCTTCAGGTGCCCAAGATGCCCCTCAAGGAGATGGAGGAGGCGGT
TCGCTGGGAAGCGGAGCGGTACATCCCCTTTCCCATTGACGAGGTGGTCCTGGACTTCGCCCCCTTAGATCCCCTGGCCG
AGGTAGCTGAGGGGGAACAGGTGGAGGTGATGGTGGGGGCAGCCCGGCAGGAAGCGGTGGCCTCTTTGCTGGAAGCCCTG
CGGGGAGCAGGCCTGACCCCCATCATTTTGGATGTAAAACCCTTTGCCGGGCTTTATCCCCTGGAAGCCCAGCTCAGCAG
CGACCCGGAAGGGGTTTCCGTGGCCGTGGAGATCGGGGCGGAAAGCACCAGCCTGGTCCTCCTTAAGGGAAACCGCCCCT
TAGCGGTGCGGATCCTCACCCTCTCCGGCAAGGACTTCACTGAAGCTATTGGGAAAAGCTTTGGCCTGGACTTCCTCACC
GCCGAGGAGGTGAAGCGCACCTACGGCCTCGCCACCATCCCCACCGAGGACGAGGAGCTTCTTCTGGACTTTGACGCCGA
GAGGGAACGGTACAGCCCCGCCAAAATCTACGATGCCATCCGGCCTGTCCTGGTGGAACTCACCCAGGAGATCCGCCGGA
GCCTGGAGTTTTTCCGGGTACAACTGGGGGACATACAACCGGAGGTAGGGTACCTCTATGGCGGGGGAAGCCGGCTTAGA
GGCCTGTCCACCCTGCTTACCGATACCCTGGGGGTCAACTTCACCGTCCCCGACCCCTGGCAGGGCATCCAGGTGGATCC
CAGACGCTTCGACCTGGAAAAGATAAAGGAGATGGAACCGGAGTTCCTGGTGCCCGTGGGCCTGGCCTTACGGGGGGTGA
TGCCCCTTGATTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-372)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Thermus thermophilus HB27

75.862

100

0.759