Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NUG20_RS16165 Genome accession   NZ_CP102592
Coordinates   3872547..3874283 (-) Length   578 a.a.
NCBI ID   WP_263395450.1    Uniprot ID   -
Organism   Xanthomonas sp. CFBP 8443     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3867547..3879283
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUG20_RS16150 (NUG20_16155) sucC 3867858..3869021 (-) 1164 WP_145702360.1 ADP-forming succinate--CoA ligase subunit beta -
  NUG20_RS16155 (NUG20_16160) - 3869303..3870916 (+) 1614 WP_263395448.1 ATP-binding protein -
  NUG20_RS16160 (NUG20_16165) pilR 3870990..3872378 (+) 1389 WP_263395449.1 sigma-54 dependent transcriptional regulator Regulator
  NUG20_RS16165 (NUG20_16170) pilB 3872547..3874283 (-) 1737 WP_263395450.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NUG20_RS16170 (NUG20_16175) - 3874435..3875292 (+) 858 WP_263395451.1 glycosyltransferase family 2 protein -
  NUG20_RS16175 (NUG20_16180) - 3875316..3876518 (+) 1203 WP_263395452.1 glycosyltransferase -
  NUG20_RS16180 (NUG20_16185) - 3876515..3877765 (+) 1251 WP_263395453.1 hypothetical protein -
  NUG20_RS16185 (NUG20_16190) - 3877740..3878831 (-) 1092 WP_263395454.1 glycosyltransferase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62778.02 Da        Isoelectric Point: 5.7952

>NTDB_id=717978 NUG20_RS16165 WP_263395450.1 3872547..3874283(-) (pilB) [Xanthomonas sp. CFBP 8443]
MNAAVSANLVGITGIARRLVQDGAVEEAVARTAMAHAAEAKIPLPQWFSEKKLATAAQLAAANAVEFGMPLLDVSVFDAN
QSAIKLVSEELLQKHQVLPLFKRGTRLFVGISNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQARSDAIGNALG
DDEEGMDNLEVGAGDEDMGSGGDTGVDAKGDDTPVVKFVNKMLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAVALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPSQLP
ENALLAEGFSAAEIAAGIQLFEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIAEAAQKIGVKDLRQSALL
KARNGITSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=717978 NUG20_RS16165 WP_263395450.1 3872547..3874283(-) (pilB) [Xanthomonas sp. CFBP 8443]
ATGAATGCAGCTGTCTCGGCCAACCTAGTTGGCATCACCGGCATCGCGCGCCGCCTGGTTCAGGATGGCGCGGTGGAGGA
GGCGGTCGCGCGGACCGCGATGGCGCATGCCGCCGAGGCCAAGATCCCGCTGCCGCAGTGGTTCTCGGAGAAGAAGCTGG
CCACCGCGGCGCAGCTGGCTGCCGCCAACGCGGTGGAGTTCGGGATGCCGCTGCTGGATGTGTCGGTGTTCGATGCCAAC
CAGAGCGCGATCAAGCTGGTCAGCGAGGAGTTGCTGCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGTACGCGGCT
GTTCGTCGGGATCAGCAATCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACCAATCTGACGGTCGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGGCGCACGCTGGAGCAGTGGCAGGCGCGCAGCGATGCGATCGGCAATGCGCTGGGC
GATGACGAGGAGGGCATGGACAACCTCGAGGTCGGGGCCGGCGACGAGGACATGGGCAGCGGCGGCGATACCGGCGTCGA
TGCCAAGGGCGACGACACGCCCGTGGTCAAGTTCGTGAACAAGATGCTGGTCGATGCAATCCGCCGCGGCGCCTCGGACA
TCCATTTCGAGCCGTACGAGGACGACTACCGGGTGCGGCTGCGCATCGATGGCCTGCTCAAGAGCGTGGCCAAGGCGCCG
GTCAAGCTCAACCAGCGCATCGCGGCGCGGCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGAGCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACGCTGTTCGGCGAGA
AGATCGTGCTGCGTATCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAACAGCAGAAG
CTGTTCTTGGACGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTATACCGCGCTGGGCATCCTCAACGACGATACCCGCAACATCTCCACCGCCGAGGATCCGGTGGAAATCCGCCTGCCCG
GCGTGAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGTGGCGCTGCGTTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCAGTTCGGTAACGCTGGTGATCGCGCAGCGCCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCCCCAGCCAGCTTCCG
GAGAATGCGCTGCTGGCCGAGGGATTCAGCGCGGCGGAAATCGCCGCCGGCATCCAGCTGTTCGAGGCCGTCGGCTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACCGGCATCTACCAGGTGATGCCGATGAACGACGAGATCGCGGCGATCGTGC
TGCAGGGCGGCAACGCGATGGACATCGCCGAGGCGGCGCAGAAGATCGGGGTCAAGGACCTGCGCCAGTCGGCGCTGCTC
AAGGCCCGCAACGGCATCACCAGCTTGGCCGAGATCAATCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-152)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.458

98.27

0.545
  pilB Acinetobacter baumannii D1279779

55.009

98.443

0.542
  pilB Legionella pneumophila strain ERS1305867

51.845

98.443

0.51
  pilF Neisseria gonorrhoeae MS11

48.768

98.27

0.479
  pilB Vibrio cholerae strain A1552

51.201

93.599

0.479
  pilB Vibrio campbellii strain DS40M4

46.221

98.443

0.455
  pilB Vibrio parahaemolyticus RIMD 2210633

45.789

98.616

0.452