Detailed information NTG0716930

Bioinformatically predicted

Overview

• Name: comM
• Type: Machinery gene
• Locus tag: NRG74_RS14125
• Genome accession: NZ_CP102428
• Coordinates: 3057358..3058422 (-)
• Length: 354 a.a.
• NCBI ID: WP_026083838.1
• Uniprot ID: A0A178L3I9
• Organism: Pseudomonas oryzihabitans strain YY7
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3052358..3063422

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NRG74_RS14100 (NRG74_14100)	aroK	3052832..3053350 (-)	519	WP_026083835.1	shikimate kinase AroK	-
NRG74_RS14105 (NRG74_14105)	pilQ	3053356..3055605 (-)	2250	WP_257704593.1	type IV pilus secretin PilQ	Machinery gene
NRG74_RS14110 (NRG74_14110)	-	3055605..3056123 (-)	519	WP_026083837.1	pilus assembly protein PilP	-
NRG74_RS14115 (NRG74_14115)	pilO	3056120..3056773 (-)	654	WP_017641025.1	type 4a pilus biogenesis protein PilO	-
NRG74_RS14120 (NRG74_14120)	-	3056770..3057357 (-)	588	WP_017641026.1	PilN domain-containing protein	-
NRG74_RS14125 (NRG74_14125)	comM	3057358..3058422 (-)	1065	WP_026083838.1	pilus assembly protein PilM	Machinery gene
NRG74_RS14130 (NRG74_14130)	-	3058590..3061010 (+)	2421	WP_017641028.1	penicillin-binding protein 1A	-
NRG74_RS14135 (NRG74_14135)	-	3061064..3062332 (-)	1269	WP_017641029.1	malic enzyme-like NAD(P)-binding protein	-
NRG74_RS14140 (NRG74_14140)	-	3062445..3063128 (-)	684	WP_257704600.1	thermonuclease family protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37797.17 Da
• Isoelectric Point: 4.6278

>NTDB_id=716930 NRG74_RS14125 WP_026083838.1 3057358..3058422(-) (comM) [Pseudomonas oryzihabitans strain YY7]
MLGLFRKKANTLLGIDVSSTSVKLIELSRAGGRYRVEAYAVEPLPANAVVEKNIAELEGVGLAIGRVLVKARSSARSAAV
AVSGSAVIAKTIEMDAGLTDDELENQIKLEADQYIPYPLDEVAIDFEVQGPSPRNAERVEVLLAACRKENVEVREAALAL
AGVSAKVVDVEAYALERAYQLLANQLGGHVDDLTVAVVDIGATMTTLSVLHNGKTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYQSEVLTPFREAVVQQVARSLQFFFAAGQYNDVDYILLAGGTASISGLDRLIQQKIGTPTVLANP
FADMTVSNKVNAAALAGDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=716930 NRG74_RS14125 WP_026083838.1 3057358..3058422(-) (comM) [Pseudomonas oryzihabitans strain YY7]
GTGCTAGGGCTATTCAGAAAGAAGGCGAATACGCTGCTGGGTATCGACGTCAGTTCGACATCCGTGAAACTCATCGAGTT
GAGCCGTGCAGGCGGGCGCTACCGGGTGGAAGCCTATGCGGTCGAACCCCTGCCGGCTAATGCCGTGGTGGAAAAGAACA
TCGCCGAGCTGGAGGGCGTGGGCCTCGCCATCGGCAGGGTGCTGGTCAAGGCGCGCAGTTCCGCGCGCAGCGCCGCCGTG
GCCGTCTCCGGCTCGGCGGTGATCGCCAAGACCATCGAGATGGATGCCGGCCTCACCGACGACGAGCTGGAAAACCAGAT
CAAGCTGGAAGCTGACCAGTACATTCCCTATCCGCTGGACGAGGTCGCCATCGACTTCGAGGTGCAGGGGCCGTCGCCAC
GCAACGCCGAGCGCGTCGAGGTGCTGCTGGCCGCTTGCCGGAAGGAAAACGTCGAGGTCCGCGAAGCGGCCCTGGCGCTG
GCCGGCGTCTCGGCCAAGGTGGTCGACGTCGAGGCCTATGCCTTGGAGCGCGCCTACCAGTTGCTGGCCAACCAGCTCGG
CGGCCACGTCGACGACCTGACCGTGGCGGTGGTCGACATCGGCGCCACCATGACCACCCTGAGCGTGCTGCACAACGGCA
AGACCATCTACACCCGCGAGCAGCTGTTTGGCGGCCGCCAGCTCACCGAAGAGATCCAGCGTCGCTATGGGCTGTCGGTG
GAGGAGGCCGGTCTGGCCAAGAAGCAGGGTGGCCTGCCCGACGACTACCAGAGCGAGGTACTGACGCCCTTCCGCGAGGC
GGTGGTGCAACAGGTGGCGCGTTCGCTGCAGTTCTTTTTCGCCGCCGGGCAATACAATGATGTGGACTACATCTTGCTTG
CTGGAGGAACCGCGTCAATTTCCGGACTCGACCGCTTGATCCAGCAGAAAATCGGCACCCCCACGGTCCTGGCCAATCCC
TTCGCCGACATGACCGTCAGCAACAAGGTCAATGCGGCGGCCCTGGCGGGTGACGCCCCGGCGCTGATGATCGCCTGCGG
CCTGGCCATGAGGAGTTTCGACTAG

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A178L3I9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comM	Acinetobacter nosocomialis M2	56.78	100	0.568
pilM	Acinetobacter baumannii D1279779	56.78	100	0.568
comM	Acinetobacter baylyi ADP1	55.367	100	0.554
pilM	Legionella pneumophila strain ERS1305867	44.633	100	0.446