Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NRG23_RS16990 Genome accession   NZ_CP102374
Coordinates   3791555..3792607 (-) Length   350 a.a.
NCBI ID   WP_257371452.1    Uniprot ID   -
Organism   Pseudomonas sp. T8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3786555..3797607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRG23_RS16965 (NRG23_16965) aroK 3787242..3787760 (-) 519 WP_009041824.1 shikimate kinase AroK -
  NRG23_RS16970 (NRG23_16970) pilQ 3787765..3789837 (-) 2073 WP_257371451.1 type IV pilus secretin PilQ Machinery gene
  NRG23_RS16975 (NRG23_16975) - 3789851..3790378 (-) 528 WP_103330911.1 pilus assembly protein PilP -
  NRG23_RS16980 (NRG23_16980) - 3790375..3790992 (-) 618 WP_009046599.1 type 4a pilus biogenesis protein PilO -
  NRG23_RS16985 (NRG23_16985) - 3790989..3791555 (-) 567 WP_009046598.1 PilN domain-containing protein -
  NRG23_RS16990 (NRG23_16990) pilM 3791555..3792607 (-) 1053 WP_257371452.1 pilus assembly protein PilM Machinery gene
  NRG23_RS16995 (NRG23_16995) - 3792824..3795259 (+) 2436 WP_164485464.1 penicillin-binding protein 1A -
  NRG23_RS17000 (NRG23_17000) - 3795387..3796655 (-) 1269 WP_009046595.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37019.45 Da        Isoelectric Point: 5.0008

>NTDB_id=716605 NRG23_RS16990 WP_257371452.1 3791555..3792607(-) (pilM) [Pseudomonas sp. T8]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLCDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGASVARTVALVDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYGLSKEEAG
LAKKQGGLPDDYASEVLQPFQEAVAQQVSRSLQFFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=716605 NRG23_RS16990 WP_257371452.1 3791555..3792607(-) (pilM) [Pseudomonas sp. T8]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCGGCCGTTTTCGGGTCGAGGCCTATGGGGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCTTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTTATCGAGATGGAGGCCGGGCTTTGCGATGACGATCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATCCCCTATCCGCTGGATGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCTTGTCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTGGAGCTC
GCCGGGCTGACGGCGCGGGTGGTGGACCTGGAGGTCTGCGCGCTGGAGCGCTCGTTCGCCCTGGCGGCCGGGGCGTCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCACCCTGAGCGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGTGGGCACCAACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGAAAGAAGAGGCCGGC
CTGGCGAAAAAGCAGGGCGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTGGCGCAGCA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCTATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTCCGGGACTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCTGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCTCCATCCTTGATGATCGCCTGTGGGCTGGCCTTGCG
GAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.114

100

0.554
  comM Acinetobacter nosocomialis M2

54.83

100

0.551
  comM Acinetobacter baylyi ADP1

52.273

100

0.526
  pilM Legionella pneumophila strain ERS1305867

45.48

100

0.46