Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NRG23_RS16970 Genome accession   NZ_CP102374
Coordinates   3787765..3789837 (-) Length   690 a.a.
NCBI ID   WP_257371451.1    Uniprot ID   -
Organism   Pseudomonas sp. T8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3782765..3794837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRG23_RS16955 (NRG23_16955) - 3784394..3785983 (-) 1590 WP_016704448.1 SPOR domain-containing protein -
  NRG23_RS16960 (NRG23_16960) aroB 3785994..3787094 (-) 1101 WP_016704447.1 3-dehydroquinate synthase -
  NRG23_RS16965 (NRG23_16965) aroK 3787242..3787760 (-) 519 WP_009041824.1 shikimate kinase AroK -
  NRG23_RS16970 (NRG23_16970) pilQ 3787765..3789837 (-) 2073 WP_257371451.1 type IV pilus secretin PilQ Machinery gene
  NRG23_RS16975 (NRG23_16975) - 3789851..3790378 (-) 528 WP_103330911.1 pilus assembly protein PilP -
  NRG23_RS16980 (NRG23_16980) - 3790375..3790992 (-) 618 WP_009046599.1 type 4a pilus biogenesis protein PilO -
  NRG23_RS16985 (NRG23_16985) - 3790989..3791555 (-) 567 WP_009046598.1 PilN domain-containing protein -
  NRG23_RS16990 (NRG23_16990) pilM 3791555..3792607 (-) 1053 WP_257371452.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74757.87 Da        Isoelectric Point: 5.3081

>NTDB_id=716604 NRG23_RS16970 WP_257371451.1 3787765..3789837(-) (pilQ) [Pseudomonas sp. T8]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRDYTTDQPARIALDLPGVVSQLANKSRD
LGNGNARSVTVVESGPRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATVAQSAPGQPAVASGRAKATASAGRSIRSVDFQ
RGEQGEGNVLIDLSDSGMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNTERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNA
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=716604 NRG23_RS16970 WP_257371451.1 3787765..3789837(-) (pilQ) [Pseudomonas sp. T8]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGCTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCGCTGCCGGGGGATCGCCTGGAGTTGAAACTCACCTTCGATGGACCGGTGCCCGAGCCGCGGG
ATTATACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCGGGTGTCGTCAGCCAGCTGGCGAACAAGAGCCGCGAT
CTGGGCAATGGCAATGCGCGCAGCGTGACGGTGGTCGAATCGGGGCCGCGTACCCGGGTGGTGGTCAATCTGGTCGCGCT
GGCGCCCTACAGCACTCGAGTCGCCGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGTGGCGCAGAGTGCAC
CGGGGCAGCCGGCTGTCGCGTCCGGGCGGGCCAAGGCCACGGCTTCGGCGGGGCGGTCGATTCGCAGTGTGGATTTCCAG
CGCGGCGAGCAGGGTGAAGGCAATGTACTGATCGACCTCTCCGACTCGGGAATGAGCCCGGATATCCAGGAACGCGACGG
CAAGCTCGTCCTTACCTTCGCCAAGGCCCGTCTGCCAGAACCCCTGCGGGTCAGGCTGGACGTCAAGGACTTCGCCACTC
CCGTGCAATTCGTCAGCGCCCGGGCGGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGCACCTTCGATTACTCG
ACCTACCAGACGGACAACAAACTCACGGTAAGTGTCAGGCCGATGACTTCGGGCGATCTGCAGAAACGCAACACCGAGCG
CTCAGCCTATAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAATCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAAAATGTGCCGTGGGACCAG
GCTCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTGGGCAATGTGTTGCTGGTGGCGCCAGCCGATGA
AATCGCGGCCCGCGAACGCCAGGAGCTGGAGTCGCAGAAGCAGATCGCCGATCTCGAGCCGCTGCGGCGTGAACTGCTGC
AGGTCAACTATGCCAAGGCGGCGGAGATCGCCAAGCTGTTCCAGTCGGTCACCCGCGCTGAGGACAAGTCTGACGAGCGT
GGTTCGATCACGGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
TGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGTGGCTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCACCAACGCC
TCTTCCACCATAGGCACGCCAGGCAGTACCAGCAGCAACTCACCGTTCGTCGACCTGGGCACCACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAACGTGTTGCTGGACCTTGAGCTGACAGCCATGGAAAAGACCGGTAACGGTGAAA
TCGTCTCCCAGCCCAAGGTGGTCACCTCCGACAAGGAGACGGCGAAGATCCTCAAGGGTACGGAAATCCCCTATCAGGAA
GCCGCCTCCAGCGGGGCGACTTCGGTGTCGTTCAAGGAGGCTTCGCTGTCCCTGGAGGTGACGCCGCAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGACGTGCCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTCCTGATCAACGACGGAGAAACCATCGTTATCGGTGGGGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTAGGCGATGTGCCGTATCTTGGCCGCCTTTTCAGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

AMIN domain (AMIN)

(40-122)

Secretin and TonB N terminus short domain (STN)

(298-344)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.769

100

0.733
  comQ Acinetobacter baylyi ADP1

39.617

100

0.42
  pilQ Acinetobacter baumannii strain A118

37.55

100

0.404
  pilQ Acinetobacter baumannii D1279779

37.55

100

0.404
  pilQ Legionella pneumophila strain Lp02

38.039

97.536

0.371
  pilQ Legionella pneumophila strain ERS1305867

38.117

96.957

0.37