Detailed information NTG0716187

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NRL37_RS01905
• Genome accession: NZ_CP102328
• Coordinates: 382359..383504 (+)
• Length: 381 a.a.
• NCBI ID: WP_044403589.1
• Uniprot ID: A0AB36F620
• Organism: Metapseudomonas otitidis strain BC12
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 377359..388504

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NRL37_RS01875 (NRL37_01875)	-	377448..377744 (-)	297	WP_172432360.1	DUF167 domain-containing protein	-
NRL37_RS01880 (NRL37_01880)	-	377744..378337 (-)	594	WP_107330252.1	YggT family protein	-
NRL37_RS01885 (NRL37_01885)	-	378429..379616 (-)	1188	WP_268193840.1	hypothetical protein	-
NRL37_RS01890 (NRL37_01890)	proC	379619..380437 (-)	819	WP_074967198.1	pyrroline-5-carboxylate reductase	-
NRL37_RS01895 (NRL37_01895)	-	380455..381147 (-)	693	WP_268193841.1	YggS family pyridoxal phosphate-dependent enzyme	-
NRL37_RS01900 (NRL37_01900)	pilT	381216..382250 (+)	1035	WP_142012818.1	type IV pilus twitching motility protein PilT	Machinery gene
NRL37_RS01905 (NRL37_01905)	pilU	382359..383504 (+)	1146	WP_044403589.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NRL37_RS27995	-	383446..384891 (-)	1446	WP_326493229.1	TIGR03915 family putative DNA repair protein	-
NRL37_RS01920 (NRL37_01920)	-	384891..386102 (-)	1212	WP_268193842.1	putative DNA modification/repair radical SAM protein	-
NRL37_RS01925 (NRL37_01925)	-	386209..386604 (+)	396	WP_074967204.1	NINE protein	-
NRL37_RS01930 (NRL37_01930)	-	386663..387934 (-)	1272	WP_268193843.1	dihydroorotase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42485.80 Da
• Isoelectric Point: 6.7727

>NTDB_id=716187 NRL37_RS01905 WP_044403589.1 382359..383504(+) (pilU) [Metapseudomonas otitidis strain BC12]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKVMPVTKNPMSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEQLKLPEVLKKLAMTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQGCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLVADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYNLYSQGEITYEDALLYADSANDLRLMIKLGSETDGEHLTSISGGLSLEVSEDDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=716187 NRL37_RS01905 WP_044403589.1 382359..383504(+) (pilU) [Metapseudomonas otitidis strain BC12]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTCGAGAAGGGCGGTTCGGACCTGTTCATCACGGCCGGTGTGCCGCCGTC
GATGAAGGTCAACGGCAAGGTCATGCCGGTCACCAAGAACCCCATGTCGCCGGAGCAGACCCGGGAAACCGTGCTGGGTG
TGATGAACGAGCAGCAGCGCCGCGACTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGGGGCATCGGCCGT
TTCCGCGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCCACCCTGGA
GCAGCTCAAGCTGCCCGAGGTGTTGAAGAAGCTGGCCATGACCAAGCGCGGCCTGGTGCTCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACGTCCCTGGCGGCGATGATCGGCTACCGCAACAAGAACAGCTCTGGCCACATCATCTCCATCGAAGAC
CCCATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGTGAAGTGGGGATCGACACCGAGTCCTTCGAAGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGGTGCGGACCCGCGAGACCATGGACCACG
CCGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCCGCCGACCGTCAGCAGCAGGTGTGGATGGACCTGTCCCTCAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGATGGCAAGGGGCGCCGCGCGGTGATCGAGGTGCTGATCAACACCCCGCTGGTGGCCGACCTGA
TCCGCAAGGGCGAGGTCCACGAGCTCAAGCCGCTGATGAAACGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAAGCG
CTGTACAACCTCTACTCCCAGGGCGAGATCACCTACGAAGACGCCCTGCTCTACGCCGACTCGGCGAACGACCTACGCCT
GATGATCAAGCTCGGTTCCGAGACCGACGGCGAACACCTGACCAGCATCAGTGGCGGGCTGTCCCTGGAAGTCTCGGAAG
ACGATCCGGGCCGTCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	92.126	100	0.921
pilU	Acinetobacter baylyi ADP1	63.866	93.701	0.598
pilU	Vibrio cholerae strain A1552	58.857	91.864	0.541
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.75	88.189	0.386