Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   NL414_RS04305 Genome accession   NZ_CP117013
Coordinates   807706..809199 (+) Length   497 a.a.
NCBI ID   WP_032230185.1    Uniprot ID   -
Organism   Escherichia coli strain MLI114     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 802706..814199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NL414_RS04285 (NL414_004285) pilD 803354..804163 (+) 810 WP_000895865.1 prepilin peptidase PppA Machinery gene
  NL414_RS04290 (NL414_004290) gspS2 804229..804639 (+) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  NL414_RS04295 (NL414_004295) gspC 804657..805616 (+) 960 WP_000135085.1 type II secretion system protein GspC -
  NL414_RS04300 (NL414_004300) gspD 805646..807706 (+) 2061 WP_000498848.1 type II secretion system secretin GspD -
  NL414_RS04305 (NL414_004305) pilB/pilB1 807706..809199 (+) 1494 WP_032230185.1 type II secretion system ATPase GspE Machinery gene
  NL414_RS04310 (NL414_004310) gspF 809199..810422 (+) 1224 WP_001173420.1 type II secretion system inner membrane protein GspF -
  NL414_RS04315 (NL414_004315) gspG 810439..810894 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  NL414_RS04320 (NL414_004320) gspH 810898..811461 (+) 564 WP_001115118.1 type II secretion system minor pseudopilin GspH -
  NL414_RS04325 (NL414_004325) gspI 811458..811829 (+) 372 WP_000820119.1 type II secretion system minor pseudopilin GspI -
  NL414_RS04330 (NL414_004330) gspJ 811826..812431 (+) 606 WP_032201846.1 type II secretion system minor pseudopilin GspJ -
  NL414_RS04335 (NL414_004335) gspK 812428..813405 (+) 978 WP_032201845.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55214.35 Da        Isoelectric Point: 5.9081

>NTDB_id=716069 NL414_RS04305 WP_032230185.1 807706..809199(+) (pilB/pilB1) [Escherichia coli strain MLI114]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGTITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCTDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=716069 NL414_RS04305 WP_032230185.1 807706..809199(+) (pilB/pilB1) [Escherichia coli strain MLI114]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGTCTTTTACGCTGAGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCAGAAGAACTACCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCAATCATCAAACTGATCAACGCCATGTTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACACTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAACGCGTACC
GCAGGATGGGCGCATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCTCGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTTCAGGCGTCGCTCACTGGGCACCT
GGTACTTTCCACACTGCATACCAACACGGCGGTGGGGACGATTACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTCCGCACGCTGTGCACCGACTGCCGTCAGCCCGCGCCTGCC
ACTGATGAAGAAAAACGCTTGCTGGGAATTACCGACGCGCGTACCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACCGCCATCCATGAGCTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGCATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTGCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Legionella pneumophila strain ERS1305867

42.612

93.964

0.4
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Acinetobacter baumannii D1279779

46.867

80.282

0.376
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

44.75

80.483

0.36
  pilB Acinetobacter baylyi ADP1

46.134

78.068

0.36